LOCUS Exported 917 bp ds-DNA linear SYN 11-JUN-2021 DEFINITION Expresses humanized PcCpf1. ACCESSION . VERSION . KEYWORDS pY017 (pcDNA3.1-hPcCpf1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 917) AUTHORS Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F TITLE Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. JOURNAL Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PUBMED 26422227 REFERENCE 2 (bases 1 to 917) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 11, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..917 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(50..130) /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA" CDS complement(137..184) /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" ORIGIN 1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg 61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat 121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc 181 ttttgtcctt ctcgtaggat ctctcctgca caaactgcag ccactccttg ttagagatgg 241 ccagcttcag cttgccgccc tcggaggtct gtctgatctg cctcagtgcc cacaggccct 301 tcagggcgat attataggct ccgtttgcat ctgcgtcctt gggcaggctc ttattgccct 361 cgcgtgtgtc aaagaacctg ccatcggcgc cggccactgg agagatcagg taatccaggt 421 ccttctcctt ccaggagttt ctcatctgca cggtcagctt gaacagcttc agcaggtcca 481 caaagaaatc cttctgcttc tcgtccacga tggcggtttt caggtcggcg gtataatcga 541 tctcgtaccg cacaaacagg gacttgaagg cctcggtcag ggcaaactcc tcgctatccc 601 actggccatt cttctgagaa tttctgaagt tcttgatccg ggagccgtgg gtgcacagaa 661 tccacatgct cctagagccc tcggccttct tggtgaagtt cttatagtca aaggcgaact 721 caaaccaatc cttcttaggg ttgtaggaga tgctgtcgaa cttctgaaag aagctcttgg 781 ccttatccac attctcgtac tgcacgtgaa acaggttcac gaagcctgtg gttgggtcga 841 tattggatgt gttccaggca gggatataga acagaaagcc gttctgcttg cccatctcct 901 taaagctctt gaaaggg //