LOCUS Exported 948 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Cas9 activity reporter (control) with BFP and GFP.. ACCESSION . VERSION . KEYWORDS pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 948) AUTHORS Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K TITLE A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. JOURNAL Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. PUBMED 27760321 REFERENCE 2 (bases 1 to 948) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..948 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(65..784) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(68..784) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(110..131) /label=EGFP-C /note="EGFP, forward primer" primer_bind 459..478 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 718..739 /label=EGFP-N /note="EGFP, reverse primer" CDS complement(791..844) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" ORIGIN 1 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgagcgg 61 ccgcttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca 121 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca 181 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt 241 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga 301 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca 361 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt 421 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct 481 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt 541 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca 601 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct 661 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt 721 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca 781 ccatgaattc aggtccgggg ttctcttcca cgtcgccgca ggtgagcagg gagccccttc 841 cttccccgcc gctgccccct ccattaagct tgtgccccag tttgctaggg aggtcgcagt 901 atctggccac tgccacctcg tgctgctcga cgtaggtctc gttgttgg //