LOCUS       Exported               11127 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  ColK-based vector; provides a nicking function to permit conjugal 
            transfer and bears a polylinker for insertion of methylase-encoding 
            genes.
ACCESSION   .
VERSION     .
KEYWORDS    pRL542
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP
  TITLE     Reduction of conjugal transfer efficiency by three restriction 
            activities of Anabaena sp. strain PCC 7120.
  JOURNAL   J Bacteriol. 1997 Mar;179(6):1998-2005.
  PUBMED    9068647
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     4232..4251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4472..4489
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4730..4752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcatcga tatctagatc
       61 tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc ggtttaggct
      121 ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt caggtaagat
      181 acttcggatg aggagcaaaa aggtggttta tacttcctat acccaaaaag gtggtttata
      241 cttcctatac cccggttatc cggtggtttt ctgtttgcgg aataaaacag attagtgaca
      301 gttttctcag aatggcgtgt tccggtgact tctgttttcg tgtgctgcat ccggaagtgt
      361 caggcccgcc ggatatgaaa aaggcctccg gagagtaccg tgactttatt ttcggcacaa
      421 atacaggggt cgatggataa atacggcgat agtttcctga cggatgatcc gtatgtaccg
      481 gcggaagaca agctgcaaac ctgtcagatg gagattgatt taatggcgga tgtgctgaga
      541 gcaccgcccc gtgaatccgc agaactgatc cgctatgtgt ttgcggatga ttggccggaa
      601 taaataaagc cgggcttaat acagattaag cccgtatagg gtattattac tgaataccaa
      661 acagcttacg gaggacggaa tgttacccat tgagacaacc agactgcctt ctgattatta
      721 atatttttca ctattaatca gaaggaataa ccatgaattt tacccggatt gacctgaata
      781 cctggaatcg cagggaacac tttgcccttt atcgtcagca gattaaatgc ggattcagcc
      841 tgaccaccaa actcgatatt accgctttgc gtaccgcact ggcggagaca ggttataagt
      901 tttatccgct gatgatttac ctgatctccc gggctgttaa tcagtttccg gagttccgga
      961 tggcactgaa agacaatgaa cttatttact gggaccagtc agacccggtc tttactgtct
     1021 ttcataaaga aaccgaaaca ttctctgcac tgtcctgccg ttattttccg gatctcagtg
     1081 agtttatggc aggttataat gcggtaacgg cagaatatca gcatgatacc agattgtttc
     1141 cgcagggaaa tttaccggag aatcacctga atatatcatc attaccgtgg gtgagttttg
     1201 acggatttaa cctgaacatc accggaaatg atgattattt tgccccggtt tttacgatgg
     1261 caaagtttca gcaggaaggt gaccgcgtat tattacctgt ttctgtacag gttcatcatg
     1321 cagtctgtga tggctttcat gcagcacggt ttattaatac acttcagctg atgtgtgata
     1381 acatactgaa ataaattaat taattctgta tttaagccac cgtatccggc aggaatggtg
     1441 gctttttttt atattttaac cgtaatctgt aatttcgttt cagactggtt caggatcact
     1501 gtacgataat gcccccgcag tttggtaata cccttaataa aaaagaaaca gcaaagactg
     1561 acagcaataa taataaagta agcagtaaca ataatattaa caacaccaga tgcagttata
     1621 ataatagtat ttaagacacc agaaagactg ctgcgacagt cattttgaac aacaccaaaa
     1681 tgccgtaaag gcagtagtaa caacaccagt gaaaacatca cgatagcata gtgatatgcc
     1741 tgagtgtgtg taattaaaca ataaataaac cgccatatat aacagaagat agtattctga
     1801 atggcatgct tttctgttca gtataaacat atcatcccgg ttggtataag gatgatatat
     1861 aataagttaa gctgaacaca tatttatttt ggttttattt tacaaataaa gtaagacgat
     1921 ccgttaagtc aaagcggggt atatttatta taccctgctt ttttatttgt ccgccgggcg
     1981 cggataatgg atcagattat gcagtgtcac aatggcctta ccgggattgg cgtaagcgtg
     2041 cgggatatcc gcatggaagc gcagggattc cccggcagaa acggtgtgcc actcatcccc
     2101 cagccgcagt tgtaatgcgc cttccagtac aatgacatgt tctctggttc tgaaatccat
     2161 ccctgtcggt gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat
     2221 tgcctggttg cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat
     2281 aattgctgcc tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc
     2341 atggtgacgg tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg
     2401 cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac
     2461 gtcgtggatg tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat
     2521 gagatcagac gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca
     2581 atcgacagct tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg
     2641 aacgatatcc aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc
     2701 agatggaatg cgctttgtta cgccagtcag aacaatcaat gccggaccga accgcaaata
     2761 ctttggtaat aacagaggga tcacctggta caactttgtg tccgatcagt attccggctt
     2821 tcatggcatc gttataccgg ggacgctgag ggactctatc tttgtgctgg aaggccttct
     2881 ggaacaggag accgggctga atccaaccga aattatgacc gatacagcag gtaccagcga
     2941 acttgtcttt ggccttttct ggctgctggg ataccagttt tctccacgcc tggctgatgc
     3001 cggtgcttcg gttttctggc gaatgggcca tgatgccaac tatggcgtgc tgaatgatat
     3061 tgccagaggg caatcagatc cccgaaaaat agtccttcag tgggacgaaa tgatccggac
     3121 cgctggctcc ctgaaactgg gcaaagtaca ggcttcagtg ctggtccgtt cattgctgaa
     3181 aagtgaacgt ccttccggac tgactcaggc aatcattgaa gtggggcgca tcaacaaaac
     3241 gctgtatctg cttaattata ttgatgatga agattaccgc cggcgcattc tgacccagct
     3301 taatcgggga gaaagccgcc atgccgttgc cagagccatc tgtcacggtc aaaaaggtga
     3361 gataagaaaa cgatataccg acggtcagga agatcaactg ggcgcactgg ggctggtcac
     3421 taacgccgtc gtgttatgga acactatgta tatgcaggca gccctggatc atctccgggc
     3481 gcaaggtgaa acactgaatg atgaagatat cgcacgcctc tccccgcttt gccacggaca
     3541 tatcaatatg ctcggccatt attccttcac gctggcagaa ctggtgacca aaggacatct
     3601 gagaccatta aaagaggcgt cagaggtaga aaacgttgct taacgtgagt tttcgttcca
     3661 ctgagcgtca gacccctgta caaggaggaa ccgttgcacc atcttcgtct tctgaactga
     3721 cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3781 cgtaatctgc tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt cgaaggttaa
     3841 gtcagttggg gactgcttaa ccgggtaact ggctttagtg gagcacagat accaaatgct
     3901 gtccttccag tgtagccgct ataaggcttc aacttcaaga gttctgcgta tctaaatcca
     3961 ctatccagtt accaatggct gctgccagtg gcgttaagtc gtgtcttacc gggttggact
     4021 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4081 agcccagctt ggagcgaacg acctacaccg aactgagata ccaacagcgt gagctatgag
     4141 aaagcgccac gcttcccaaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4201 gaacaggaga gcgcacgagg gagccaccag ggggaaacgc ctggtatctt taagtcctgt
     4261 cgggtttcgc cacctctgac ttgagcgtcg gtttttgtga tgctcgtcag gggggcggag
     4321 cctatggaaa aacgccagct gcgtggcctt ttccgggttc ttgttttttg ctcacatgtt
     4381 tcttttggtg ttatcccctg attctgtgga taaccgcatt accgtctttg agtgagctga
     4441 taccgctcgc cgcagccgaa cgaccgagcg gagcgagtca gtgagcgagg aagcggaaaa
     4501 gcgcctggac gagcattttc tccttacgca tctgtgcggc atttcacacc cggcatggtg
     4561 cacttttcat acaatcctca ctgatgccaa atgattaagc cagtatacac tccgctatcg
     4621 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     4681 cgggcttgtc tgctcccggc atccgctcac aggccagctg tgaccgtctc cgggagctgc
     4741 atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     4801 tcagcgtggt cgtgaagcgc ttccagatgt cagcctggtc gtcggcgtcc agttcattca
     4861 gtttttccag cagggtcagc gtctggcttc tgatgaagcg ggccatgtta agggcggttt
     4921 tttcctgttt agtcacagtt ctgtttctcc atgtgtgggg agtctgtcgg aggtttttaa
     4981 tgataccggt gaaacgcgaa aggatgctca cgatacgggt cacggatgag gaacatgcgc
     5041 ggctgctggc acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag gtctgcctgg
     5101 ggcaccgcca tcgaaaacgt caggtcttcc gacactggca ccgccgctgt tgcgccagtt
     5161 cgcttcggtc ggcaataacc tgaatcagat agcccggaag ataaacagcg gacagtggtc
     5221 ggggcatgac cgggttcatg tggtggctgc actgatggcc atcggacgtg aactgagtga
     5281 gctgagggat gaggtccgta aacagggaga acgggatgat agttaagttt catgccaggg
     5341 gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt aaccgggaag
     5401 gggcgagagt tctgcggggt gctcccgaag aggtgcggga actcatcgat gccacgccgt
     5461 ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagaccctg ccaccgggag
     5521 agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtctt gaaaaaaatc
     5581 agtacagcat cctgtgggtg gagcaccagg acaaggggcg tcttgagctg aattttgtca
     5641 tcccgaatat ggagctggcg agcggaaaac ggctccagcc ctattacgac cgagcggacc
     5701 gtccgcgaat caatgcctgg cagacactgg tgaatcacca ttacgggctg cacgacccga
     5761 acgcaccgga gaacaggcgg acacttgtca ccccgaataa tctgccgaaa gcgaaacagg
     5821 aggccgcaga agcgattacg aggggactgg aggctcttta ccatgccgga gcgctaaaaa
     5881 cgcgtcagga tgtcactgag gcgcttacag cggcaggttt tgaggttgtg aggacaacca
     5941 ggagcagtat cagtatcgcc gacccggagg gtgggcgtaa tctcaggctg aaaggggcgc
     6001 tgtatgagca atcttttaca aatggggaca gacttcgaga agaaactgaa agagcgtgcc
     6061 gtctctacag agaacatgct gaacagcgag ttcaggaagc tcgaagcgtc tgtagacagg
     6121 gaactgaact taaacgggca gaaaatcaga gacgctatca gcgcacacac agcagcggtg
     6181 aaagaacaac tggagacgct gaacagtgcc gtgaacgcac agttcagcgc cacagagaac
     6241 gaactggccc ggcggcagga agagctgctc tggaagctgg tcaaagggcg gatactgtac
     6301 ccgtcgctga cggcgctttg cgtcacgggc gggatttttc tggcgtcatg ggggctgatt
     6361 cagtggcagg aaagccggat tgcgggaaac atcctggcta tcagggacca ggaagagacg
     6421 ctggcgaaac tccgggaaaa aacatggggt gtgacgtatc acgagggcag gaacgggaaa
     6481 tttctggtgc tgccgtccgg ggtgaagggg gaaaacaact ggacggtgga gaagaagaac
     6541 gcagtaagac tggtcaggga gtaagtgagt atgacagagc tggaaatgca cttactgaac
     6601 gccttagagc aattgcagca ggactatacg cagcggctga aagactggga gagccccttc
     6661 acggatttgc agcatatgtt cgagctgaca cgacaggaga acgggcaact gagggaacag
     6721 tgcgacgact tgagtcagca ggtgcagaga ttagccgggc aggtggacca cttgagtcgg
     6781 ttatttatca cgaacagcag ataagggatg aacaggaaag gcaggcacgc ctggaacggg
     6841 agcgtgcgcg gtcctggcat gaagagcagg aacgggaagc acgggtatgg cgtggcccgt
     6901 caatagggat gtgacggtga gttgttccgg gaggttgcca gatggtaccg gaacgcgtta
     6961 aaaacgcatc cgggcgcgat tctggcgtgt cgtttttgtt gctgtgtttt gcctctttgc
     7021 cagatcgcgc tcattcctca gcctcatccc gcgatgtctg cgtatgtcat caactgaaaa
     7081 gatggaaaaa aaacagtgtc atgaggttta cgcaggcaaa accagcgtta ttcacatggc
     7141 tgaatagcta tgcatacggt taaaatttat caggtgcgat cgcggcagct tttcgggtgg
     7201 tttgttgcca tttttacctg tcagcagccg tgatcgcgct gaacgcattt cagcggtgcg
     7261 tacaattaag ggattatggt aaatccctta ctgtctgccc tcgtatccat cgagataaac
     7321 cgcagaaatg gtgtctgcca ttagtttcat ttttttcggc gaagcatatc gctgtttgtg
     7381 tattttctgc cagcaaaggc tgttgtgctc cggagtgagt gaaaaaattt ttctgagtgg
     7441 aattctttta cacgttgctg tgccgtttct gctgatactc cggctgcttt gtctttcatc
     7501 cccatatccc ggaactgctg aaagaacggg agcatcatac gtatggtgtc tgctttctgg
     7561 gtgtagaagt taggatcaaa tccatcatct gaagcataaa cataatgccg ggtatgtcgt
     7621 tccaggctgg tctttggtgt gcatcctgta agagctattg ttgctgttat cagaggtatc
     7681 agaagcagcc tggcagcggc agatctcata aacatctcct tgcgttttta atcttgatac
     7741 tcagggagtt atatctccga agagtatctc ttatcatacg tcggtttact cccaatgtga
     7801 tttgatggtc aatgagtcag gaataatccc gtgaaaaaat gtgtgccagt cctgcttttt
     7861 agtgttcttt ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc
     7921 gagaagacaa aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaac
     7981 agactgaaga tacaacttca gatcgatgag ttgagagcag actggctggc accagagatg
     8041 cccaaaaaag agaggatttg cgtaaaaaac tggagtctct ggagcggcaa aagaaatcct
     8101 tacgctgatg ggggcgtatt ttccccggtt tcttccataa cctttttacc gttcagatat
     8161 ttacgggcga ggcgaatgcg tcttgcctgg tataccgccg agtaagcctg ggtgggattc
     8221 acaccattac tgatttcact gaccacatga cgctgatcag ccagtgtcag cgtgtaaacc
     8281 tgtttctgga tgaggtcacg ggctttgtca tgctcatatc cggcagcaac aagggtgtcg
     8341 agaagtcgtt tgtctgtttt gttcagttta tggcggggtg atttgctcac atcgttctcc
     8401 gttttttcat gatccaacag ttttatatct catcctgatt tgctccggtg ttctgtttcg
     8461 acgttctcaa tgcctgcctc cccatagtgt gatccttatt gcaaaaatga aaatccatgc
     8521 tcttgacatg gacaatgctg agtagtaggt ttttactgta cataaaacca gtggttatat
     8581 gtacagtata ttgtttttaa tttattgttt taattatcaa agaggaattt atggctaaag
     8641 aactaagtgg atatggacca actgctggtg agtctatggg gggaacaggg gcgaatctga
     8701 atcagcaagg tggaaataat aatagtaata gtggggttca ctggggtggt ggctctgggc
     8761 atggtaataa tggtggtcag ggaaattcaa attcatctgg ttctacatca actgtgatga
     8821 aaaccggtga gtcatatctg actccgtggg gtgatgttgt cattaataat gatggccttc
     8881 cggtgatgaa tggtattgtt atgacagaag agaattcaac tctggttgat aatccatttg
     8941 gaggggtctc cagagttctt aattctttaa ttagtgatat gccatcactt tttgcagaaa
     9001 gctcaggaaa taacaacaat aacactgctt cagttaatac tgcaccaacg aatgctcagg
     9061 tcagtgatat ggataagagt tcgaaggtgg tgggtaatgt aattaatgaa aaacaaaagc
     9121 agaagaataa aattgctaca cagattagtg aaaaacagaa gaaaatcgag gagatgaaaa
     9181 aagtattcaa gcatcattct tatcatggta tcactgatct tgagagggat gttgatgagt
     9241 tgcaaaaaaa atcaaatcag cttgatgcag atattagcaa acttaattct tataaaaata
     9301 cattgcaaag taaaattggt gatgtgaata aacagaagga agctgaagaa aaggctcgtg
     9361 aaaatgcaga ggtcgctgaa catgaaactc ttaatgaaga gaaacaggcg gtggcggaag
     9421 cagagaagcg tttagctgag gcgaaagctg aactggcgaa ggctgaaagt gatgtacaga
     9481 gtaagcaagc tactgtttcc agagttgcag gagagcttga aaacgctcag aagagtgttg
     9541 acgtgaaggt caccggattt ccgggatggc gtgacgttca gaaaaaacta cagcgtcagc
     9601 ttgaagctaa gcaggctgag tactccgcag ttgaaaatga acttaagaat gctgttagtt
     9661 tcagggatgg taaggccgct gaggttaaag aggcagagca gaaattaaaa gaagctcagg
     9721 atgctcttga aaagagccag ataaaagatg ctgttgatac tatggttgga ttttatcaat
     9781 atataaccga acaatatgga gaaaaatacg caaaaattgc tcaggattta gcggaaaagt
     9841 ctaaaggaaa gaaaattcaa ggtgttgatg aagctctggc agcatttgaa aagtataaga
     9901 atgtactgga taagaaattc agtaaggttg atcgggatgc tatttttaat gcgttagagt
     9961 ctgttaatta tgatgagtta tctaagaatt taacgaagat atcaaagtcg cttaaaatca
    10021 ccagtcgagt atcttttcta tatgatgttg gttctgattt taaaaatgca atagaaactg
    10081 gtaactggcg acctttgttt gtcacgctgg agaaaagtgc ggtcgatgta ggtgtggcaa
    10141 aaattgtggc attaatgttt agttttattg ttggtgtgcc tcttggtttc tgggggattg
    10201 caattgtcac aggtattgtt tcttcttaca taggggatga tgagttgagc aaacttaatg
    10261 agttgttagg gatttaattt ctctttagag tgtaatatat gaaacttggc aatcctactg
    10321 ggattgccat taataaataa aacaatgcag ataacttgca taagtaccag ggttcttttt
    10381 ttaactttgt attttctttg atatatctag atatggttaa tttagagtat ggatatagca
    10441 atacatgtat tgatgatata aatataaata acaaaggagt gttatcattg tcgttaaata
    10501 ttaaaataag aatccatggt ataagtgatt caggtaaatt atgtaggtag tattttaaat
    10561 gcataagtat ttttactttt gttggaaaga aatatgaaaa taataatttt tctaattaca
    10621 atattattct tgtcaggatg tcaagcaaac tatattcgtg atgtacgggg tctgacgctc
    10681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10801 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10861 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10921 taccatctgg ccccagtgct gcaatgatac cgcgagatcc acgctcaccg gctccagatt
    10981 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    11041 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    11101 atagtttgcg caacgttgtt gccattg
//
