LOCUS Exported 3674 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway ORF clone of human CDK6 [NM_001259.6] with stop codon (for native or N-terminal fusions). ACCESSION . VERSION . KEYWORDS R777-E025 Hs.CDK6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3674) TITLE Esposito lab REFERENCE 2 (bases 1 to 3674) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3674 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..45 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 37..54 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 38..54 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 84..183 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1165..1264) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 1641..2432 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2525..3113 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3014..3033 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3267..3284 /label=L4440 /note="L4440 vector, forward primer" terminator 3443..3470 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3562..3648 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa 61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt 121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg 181 gcaccatgga gaaggacggc ctgtgccgcg ctgaccagca gtacgaatgc gtggcggaga 241 tcggggaggg cgcctatggg aaggtgttca aggcccgcga cttgaagaac ggaggccgtt 301 tcgtggcgtt gaagcgcgtg cgggtgcaga ccggcgagga gggcatgccg ctctccacca 361 tccgcgaggt ggcggtgctg aggcacctgg agaccttcga gcaccccaac gtggtcaggt 421 tgtttgatgt gtgcacagtg tcacgaacag acagagaaac caaactaact ttagtgtttg 481 aacatgtcga tcaagacttg accacttact tggataaagt tccagagcct ggagtgccca 541 ctgaaaccat aaaggatatg atgtttcagc ttctccgagg tctggacttt cttcattcac 601 accgagtagt gcatcgcgat ctaaaaccac agaacattct ggtgaccagc agcggacaaa 661 taaaactcgc tgacttcggc cttgcccgca tctatagttt ccagatggct ctaacctcag 721 tggtcgtcac gctgtggtac agagcacccg aagtcttgct ccagtccagc tacgccaccc 781 ccgtggatct ctggagtgtt ggctgcatat ttgcagaaat gtttcgtaga aagcctcttt 841 ttcgtggaag ttcagatgtt gatcaactag gaaaaatctt ggacgtgatt ggactcccag 901 gagaagaaga ctggcctaga gatgttgccc ttcccaggca ggcttttcat tcaaaatctg 961 cccaaccaat tgagaagttt gtaacagata tcgatgaact aggcaaagac ctacttctga 1021 agtgtttgac atttaaccca gccaaaagaa tatctgccta cagtgccctg tctcacccat 1081 acttccagga cctggaaagg tgcaaagaaa acctggattc ccacctgccg cccagccaga 1141 acacctcgga gctgaataca gcctaatcaa ctttcttgta caaagttggc attataggaa 1201 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 1261 tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg acttcgctgc 1321 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga 1381 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt 1441 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt 1501 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 1561 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc 1621 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat 1681 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 1741 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 1801 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 1861 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 1921 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 1981 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 2041 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 2101 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 2161 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 2221 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 2281 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac 2341 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga 2401 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc 2461 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 2521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 2581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 2641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 2701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 2761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 2821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 2881 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg 2941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 3001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 3061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 3121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 3181 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 3241 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 3301 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 3361 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc 3421 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg 3481 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac 3541 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca 3601 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt 3661 tccctactct cgcg //