LOCUS       Exported                6323 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway ORF clone of human EGFR [NM_005228.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    R777-E054 Hs.EGFR-nostop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6323)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             186..3815
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(3814..3913)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4290..5081
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5174..5762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5663..5682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5916..5933
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      6092..6119
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      6211..6297
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcg accctccggg acggccgggg cagcgctcct ggcgctgctg gctgcgctct
      241 gcccggcgag tcgggctctg gaggaaaaga aagtttgcca aggcacgagt aacaagctca
      301 cgcagttggg cacttttgaa gatcattttc tcagcctcca gaggatgttc aataactgtg
      361 aggtggtcct tgggaatttg gaaattacct atgtgcagag gaattatgat ctttccttct
      421 taaagaccat ccaggaggtg gctggttatg tcctcattgc cctcaacaca gtggagcgaa
      481 ttcctttgga aaacctgcag atcatcagag gaaatatgta ctacgaaaat tcctatgcct
      541 tagcagtctt atctaactat gatgcaaata aaaccggact gaaggagctg cccatgagaa
      601 atttacagga aatcctgcat ggcgccgtgc ggttcagcaa caaccctgcc ctgtgcaacg
      661 tggagagcat ccagtggcgg gacatagtca gcagtgactt tctcagcaac atgtcgatgg
      721 acttccagaa ccacctgggc agctgccaaa agtgtgatcc aagctgtccc aatgggagct
      781 gctggggtgc aggagaggag aactgccaga aactgaccaa aatcatctgt gcccagcagt
      841 gctccgggcg ctgccgtggc aagtccccca gtgactgctg ccacaaccag tgtgctgcag
      901 gctgcacagg cccccgggag agcgactgcc tggtctgccg caaattccga gacgaagcca
      961 cgtgcaagga cacctgcccc ccactcatgc tctacaaccc caccacgtac cagatggatg
     1021 tgaaccccga gggcaaatac agctttggtg ccacctgcgt gaagaagtgt ccccgtaatt
     1081 atgtggtgac agatcacggc tcgtgcgtcc gagcctgtgg ggccgacagc tatgagatgg
     1141 aggaagacgg cgtacgtaag tgtaagaagt gcgaagggcc ttgccgcaaa gtgtgtaacg
     1201 gaataggtat tggtgaattt aaagactcac tctccataaa tgctacgaat attaaacact
     1261 tcaaaaactg cacctccatc agtggcgatc tccacatcct gccggtggca tttaggggtg
     1321 actccttcac acatactcct cctctggatc cacaggaact ggatattctg aaaaccgtaa
     1381 aggaaatcac agggtttttg ctgattcagg cttggcctga aaacaggacg gacctccatg
     1441 cctttgagaa cctagaaatc atacgcggca ggaccaagca acatggtcag ttttctcttg
     1501 cagtcgtcag cctgaacata acatccttgg gattacgctc cctcaaggag ataagtgatg
     1561 gagatgtgat aatttcagga aacaaaaatt tgtgctatgc aaatacaata aactggaaaa
     1621 aactgtttgg gacctccggt cagaaaacca aaattataag caacagaggt gaaaacagct
     1681 gcaaggccac aggccaggtc tgccacgcgt tgtgctcccc cgagggctgc tggggcccgg
     1741 agcccaggga ctgcgtctct tgccggaatg tcagccgagg cagggaatgc gtggacaagt
     1801 gcaaccttct ggagggtgag ccaagggagt ttgtggagaa ctctgagtgc atacagtgcc
     1861 acccagagtg cctgcctcag gccatgaaca tcacctgcac aggacgggga ccagacaact
     1921 gtatccagtg tgcccactac attgacggcc cccactgcgt caagacctgc ccggcaggag
     1981 tcatgggaga aaacaacacc ctggtctgga agtacgcaga cgccggccat gtgtgccacc
     2041 tgtgccatcc aaactgcacc tacggatgca ctgggccagg tcttgaaggc tgtccaacga
     2101 atgggcctaa gatcccgtcc atcgccactg ggatggtggg ggccctcctc ttgctgctgg
     2161 tggtggccct ggggatcggc ctcttcatgc gaaggcgcca catcgttcgg aagcgcacgc
     2221 tgcggaggct gctgcaggag agggagcttg tggagcctct tacacccagt ggagaagctc
     2281 ccaaccaagc tctcttgagg atcttgaagg aaactgaatt caaaaagatc aaagtgctgg
     2341 gctccggtgc gttcggcacg gtgtataagg gactctggat cccagaaggt gagaaagtta
     2401 aaattcccgt cgctatcaag gaattaagag aagcaacatc tccgaaagcc aacaaggaaa
     2461 tcctcgatga agcctacgtg atggccagcg tggacaaccc ccacgtgtgc cgcctgctgg
     2521 gcatctgcct cacctccacc gtgcaactca tcacgcagct catgcccttc ggctgcctcc
     2581 tggactatgt ccgggaacac aaagacaata ttggctccca gtacctgctc aactggtgtg
     2641 tgcagatcgc aaagggcatg aactacttgg aggaccgtcg cttggtgcac cgcgacctgg
     2701 cagccaggaa cgtactggtg aaaacaccgc agcatgtcaa gatcacagat tttgggctgg
     2761 ccaaactgct gggtgcggaa gagaaagaat accatgcaga aggaggcaaa gtgcctatca
     2821 agtggatggc attggaatca attttacaca gaatctatac ccaccagagt gatgtctgga
     2881 gctacggggt gaccgtttgg gagttgatga cctttggatc caagccatat gacggaatcc
     2941 ctgccagcga gatctcctcc atcctggaga aaggagaacg cctccctcag ccacccatat
     3001 gtaccatcga tgtctacatg atcatggtca agtgctggat gatagacgca gatagtcgcc
     3061 caaagttccg tgagttgatc atcgaattct ccaaaatggc ccgagacccc cagcgctacc
     3121 ttgtcattca gggggatgaa agaatgcatt tgccaagtcc tacagactcc aacttctacc
     3181 gtgccctgat ggatgaagaa gacatggacg acgtggtgga tgccgacgag tacctcatcc
     3241 cacagcaggg cttcttcagc agcccctcca cgtcacggac tcccctcctg agctctctga
     3301 gtgcaaccag caacaattcc accgtggctt gcattgatag aaatgggctg caaagctgtc
     3361 ccatcaagga agacagcttc ttgcagcgat acagctcaga ccccacaggc gccttgactg
     3421 aggacagcat agacgacacc ttcctcccag tgcctgaata cataaaccag tccgttccca
     3481 aaaggcccgc tggctctgtg cagaatcctg tctatcacaa tcagcctctg aaccccgcgc
     3541 ccagcagaga cccacactac caggaccccc acagcactgc agtgggcaac cccgagtatc
     3601 tcaacactgt ccagcccacc tgtgtcaaca gcacattcga cagccctgcc cactgggccc
     3661 agaaaggcag ccaccaaatt agcctggaca accctgacta ccagcaggac ttctttccca
     3721 aggaagccaa gccaaatggc atctttaagg gctccacagc tgaaaatgca gaatacctaa
     3781 gggtcgcgcc acaaagcagt gaatttattg gagcatcaac tttcttgtac aaagttggca
     3841 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     3901 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     3961 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     4021 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     4081 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     4141 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     4201 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     4261 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     4321 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     4381 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     4441 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     4501 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     4561 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     4621 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     4681 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4741 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4801 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4861 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4921 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4981 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     5041 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     5101 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     5161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5281 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     5581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5941 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6001 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     6061 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     6121 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     6181 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     6241 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     6301 cctggcagtt ccctactctc gcg
//
