LOCUS       Exported                6461 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway ORF clone of human ERBB2 [NM_004448.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    R777-E061 Hs.ERBB2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3952..4051)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4428..5219
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5312..5900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5801..5820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6054..6071
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      6230..6257
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      6349..6435
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gctggcggcc ttgtgccgct gggggctcct cctcgccctc ttgccccccg
      241 gagccgcgag cacccaagtg tgcaccggca cagacatgaa gctgcggctc cctgccagtc
      301 ccgagaccca cctggacatg ctccgccacc tctaccaggg ctgccaggtg gtgcagggaa
      361 acctggaact cacctacctg cccaccaatg ccagcctgtc cttcctgcag gatatccagg
      421 aggtgcaggg ctacgtgctc atcgctcaca accaagtgag gcaggtccca ctgcagaggc
      481 tgcggattgt gcgaggcacc cagctctttg aggacaacta tgccctggcc gtgctagaca
      541 atggagaccc gctgaacaat accacccctg tcacaggggc ctccccagga ggcctgcggg
      601 agctgcagct tcgaagcctc acagagatct tgaaaggagg ggtcttgatc cagcggaacc
      661 cccagctctg ctaccaggac acgattttgt ggaaggacat cttccacaag aacaaccagc
      721 tggctctcac actgatagac accaaccgct ctcgggcctg ccacccctgt tctccgatgt
      781 gtaagggctc ccgctgctgg ggagagagtt ctgaggattg tcagagcctg acgcgcactg
      841 tctgtgccgg tggctgtgcc cgctgcaagg ggccactgcc cactgactgc tgccatgagc
      901 agtgtgctgc cggctgcacg ggccccaagc actctgactg cctggcctgc ctccacttca
      961 accacagtgg catctgtgag ctgcactgcc cagccctggt cacctacaac acagacacgt
     1021 ttgagtccat gcccaatccc gagggccggt atacattcgg cgccagctgt gtgactgcct
     1081 gtccctacaa ctacctttct acggacgtgg gatcctgcac cctcgtctgc cccctgcaca
     1141 accaagaggt gacagcagag gatggaacac agcggtgtga gaagtgcagc aagccctgtg
     1201 cccgagtgtg ctatggtctg ggcatggagc acttgcgaga ggtgagggca gttaccagtg
     1261 ccaatatcca ggagtttgct ggctgcaaga agatctttgg gagcctggca tttctgccgg
     1321 agagctttga tggggaccca gcctccaaca ctgccccgct ccagccagag cagctccaag
     1381 tgtttgagac tctggaagag atcacaggtt acctatacat ctcagcatgg ccggacagcc
     1441 tgcctgacct cagcgtcttc cagaacctgc aagtaatccg gggacgaatt ctgcacaatg
     1501 gcgcctactc gctgaccctg caagggctgg gcatcagctg gctggggctg cgctcactga
     1561 gggaactggg cagtggactg gccctcatcc accataacac ccacctctgc ttcgtgcaca
     1621 cggtgccctg ggaccagctc tttcggaacc cgcaccaagc tctgctccac actgccaacc
     1681 ggccagagga cgagtgtgtg ggcgagggcc tggcctgcca ccagctgtgc gcccgagggc
     1741 actgctgggg tccagggccc acccagtgtg tcaactgcag ccagttcctt cggggccagg
     1801 agtgcgtgga ggaatgccga gtactgcagg ggctccccag ggagtatgtg aatgccaggc
     1861 actgtttgcc gtgccaccct gagtgtcagc cccagaatgg ctcagtgacc tgttttggac
     1921 cggaggctga ccagtgtgtg gcctgtgccc actataagga ccctcccttc tgcgtggccc
     1981 gctgccccag cggtgtgaaa cctgacctct cctacatgcc catctggaag tttccagatg
     2041 aggagggcgc atgccagcct tgccccatca actgcaccca ctcctgtgtg gacctggatg
     2101 acaagggctg ccccgccgag cagagagcca gccctctgac gtccatcatc tctgcggtgg
     2161 ttggcattct gctggtcgtg gtcttggggg tggtctttgg gatcctcatc aagcgacggc
     2221 agcagaagat ccggaagtac acgatgcgga gactgctgca ggaaacggag ctggtggagc
     2281 cgctgacacc tagcggagcg atgcccaacc aggcgcagat gcggatcctg aaagagacgg
     2341 agctgaggaa ggtgaaggtg cttggatctg gcgcttttgg cacagtctac aagggcatct
     2401 ggatccctga tggggagaat gtgaaaattc cagtggccat caaagtgttg agggaaaaca
     2461 catcccccaa agccaacaaa gaaatcttag acgaagcata cgtgatggct ggtgtgggct
     2521 ccccatatgt ctcccgcctt ctgggcatct gcctgacatc cacggtgcag ctggtgacac
     2581 agcttatgcc ctatggctgc ctcttagacc atgtccggga aaaccgcgga cgcctgggct
     2641 cccaggacct gctgaactgg tgtatgcaga ttgccaaggg gatgagctac ctggaggatg
     2701 tgcggctcgt acacagggac ttggccgctc ggaacgtgct ggtcaagagt cccaaccatg
     2761 tcaaaattac agacttcggg ctggctcggc tgctggacat tgacgagaca gagtaccatg
     2821 cagatggggg caaggtgccc atcaagtgga tggcgctgga gtccattctc cgccggcggt
     2881 tcacccacca gagtgatgtg tggagttatg gtgtgactgt gtgggagctg atgacttttg
     2941 gggccaaacc ttacgatggg atcccagccc gggagatccc tgacctgctg gaaaaggggg
     3001 agcggctgcc ccagcccccc atctgcacca ttgatgtcta catgatcatg gtcaaatgtt
     3061 ggatgattga ctctgaatgt cggccaagat tccgggagtt ggtgtctgaa ttctcccgca
     3121 tggccaggga cccccagcgc tttgtggtca tccagaatga ggacttgggc ccagccagtc
     3181 ccttggacag caccttctac cgctcactgc tggaggacga tgacatgggg gacctggtgg
     3241 atgctgagga gtatctggta ccccagcagg gcttcttctg tccagaccct gccccgggcg
     3301 ctgggggcat ggtccaccac aggcaccgca gctcatctac caggagtggc ggtggggacc
     3361 tgacactagg gctggagccc tctgaagagg aggcccccag gtctccactg gcaccctccg
     3421 aaggggctgg ctccgatgta tttgatggtg acctgggaat gggggcagcc aaggggctgc
     3481 aaagcctccc cacacatgac cccagccctc tacagcggta cagtgaggac cccacagtac
     3541 ccctgccctc tgagactgat ggctacgttg cccccctgac ctgcagcccc cagcctgaat
     3601 atgtgaacca gccagatgtt cggccccagc ccccttcgcc ccgagagggc cctctgcctg
     3661 ctgcccgacc tgctggtgcc actctggaaa ggcccaagac tctctcccca gggaagaatg
     3721 gggtcgtcaa agacgttttt gcctttgggg gtgccgtgga gaaccccgag tacttgacac
     3781 cccagggagg agctgcccct cagccccacc ctcctcctgc cttcagccca gccttcgaca
     3841 acctctatta ctgggaccag gacccaccag agcggggggc tccacccagc accttcaaag
     3901 ggacacctac ggcagagaac ccagagtacc tgggtctgga cgtgccagtg taatcaactt
     3961 tcttgtacaa agttggcatt ataggaaagc attgcttatc aatttgttgc aacgaacagg
     4021 tcactatcag tcaaaataaa atcattattt gcatgcaagc ttagatcctc tagaccagcc
     4081 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     4141 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     4201 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     4261 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     4321 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     4381 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     4441 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     4501 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     4561 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     4621 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     4681 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     4741 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     4801 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     4861 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     4921 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     4981 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     5041 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     5101 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     5161 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     5221 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     5281 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5341 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5401 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5461 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5521 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5581 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5641 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5701 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5761 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5821 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5881 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5941 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6001 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6061 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6121 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6181 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     6241 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     6301 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     6361 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     6421 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc g
//
