LOCUS Exported 6458 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway ORF clone of human ERBB2 [NM_004448.3] without stop codon (for C-terminal fusions). ACCESSION . VERSION . KEYWORDS R777-E062 Hs.ERBB2-nostop SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6458) TITLE Esposito lab REFERENCE 2 (bases 1 to 6458) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6458 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..45 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 37..54 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 38..54 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 84..183 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(3949..4048) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 4425..5216 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 5309..5897 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5798..5817 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6051..6068 /label=L4440 /note="L4440 vector, forward primer" terminator 6227..6254 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 6346..6432 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa 61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt 121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg 181 gcaccatgga gctggcggcc ttgtgccgct gggggctcct cctcgccctc ttgccccccg 241 gagccgcgag cacccaagtg tgcaccggca cagacatgaa gctgcggctc cctgccagtc 301 ccgagaccca cctggacatg ctccgccacc tctaccaggg ctgccaggtg gtgcagggaa 361 acctggaact cacctacctg cccaccaatg ccagcctgtc cttcctgcag gatatccagg 421 aggtgcaggg ctacgtgctc atcgctcaca accaagtgag gcaggtccca ctgcagaggc 481 tgcggattgt gcgaggcacc cagctctttg aggacaacta tgccctggcc gtgctagaca 541 atggagaccc gctgaacaat accacccctg tcacaggggc ctccccagga ggcctgcggg 601 agctgcagct tcgaagcctc acagagatct tgaaaggagg ggtcttgatc cagcggaacc 661 cccagctctg ctaccaggac acgattttgt ggaaggacat cttccacaag aacaaccagc 721 tggctctcac actgatagac accaaccgct ctcgggcctg ccacccctgt tctccgatgt 781 gtaagggctc ccgctgctgg ggagagagtt ctgaggattg tcagagcctg acgcgcactg 841 tctgtgccgg tggctgtgcc cgctgcaagg ggccactgcc cactgactgc tgccatgagc 901 agtgtgctgc cggctgcacg ggccccaagc actctgactg cctggcctgc ctccacttca 961 accacagtgg catctgtgag ctgcactgcc cagccctggt cacctacaac acagacacgt 1021 ttgagtccat gcccaatccc gagggccggt atacattcgg cgccagctgt gtgactgcct 1081 gtccctacaa ctacctttct acggacgtgg gatcctgcac cctcgtctgc cccctgcaca 1141 accaagaggt gacagcagag gatggaacac agcggtgtga gaagtgcagc aagccctgtg 1201 cccgagtgtg ctatggtctg ggcatggagc acttgcgaga ggtgagggca gttaccagtg 1261 ccaatatcca ggagtttgct ggctgcaaga agatctttgg gagcctggca tttctgccgg 1321 agagctttga tggggaccca gcctccaaca ctgccccgct ccagccagag cagctccaag 1381 tgtttgagac tctggaagag atcacaggtt acctatacat ctcagcatgg ccggacagcc 1441 tgcctgacct cagcgtcttc cagaacctgc aagtaatccg gggacgaatt ctgcacaatg 1501 gcgcctactc gctgaccctg caagggctgg gcatcagctg gctggggctg cgctcactga 1561 gggaactggg cagtggactg gccctcatcc accataacac ccacctctgc ttcgtgcaca 1621 cggtgccctg ggaccagctc tttcggaacc cgcaccaagc tctgctccac actgccaacc 1681 ggccagagga cgagtgtgtg ggcgagggcc tggcctgcca ccagctgtgc gcccgagggc 1741 actgctgggg tccagggccc acccagtgtg tcaactgcag ccagttcctt cggggccagg 1801 agtgcgtgga ggaatgccga gtactgcagg ggctccccag ggagtatgtg aatgccaggc 1861 actgtttgcc gtgccaccct gagtgtcagc cccagaatgg ctcagtgacc tgttttggac 1921 cggaggctga ccagtgtgtg gcctgtgccc actataagga ccctcccttc tgcgtggccc 1981 gctgccccag cggtgtgaaa cctgacctct cctacatgcc catctggaag tttccagatg 2041 aggagggcgc atgccagcct tgccccatca actgcaccca ctcctgtgtg gacctggatg 2101 acaagggctg ccccgccgag cagagagcca gccctctgac gtccatcatc tctgcggtgg 2161 ttggcattct gctggtcgtg gtcttggggg tggtctttgg gatcctcatc aagcgacggc 2221 agcagaagat ccggaagtac acgatgcgga gactgctgca ggaaacggag ctggtggagc 2281 cgctgacacc tagcggagcg atgcccaacc aggcgcagat gcggatcctg aaagagacgg 2341 agctgaggaa ggtgaaggtg cttggatctg gcgcttttgg cacagtctac aagggcatct 2401 ggatccctga tggggagaat gtgaaaattc cagtggccat caaagtgttg agggaaaaca 2461 catcccccaa agccaacaaa gaaatcttag acgaagcata cgtgatggct ggtgtgggct 2521 ccccatatgt ctcccgcctt ctgggcatct gcctgacatc cacggtgcag ctggtgacac 2581 agcttatgcc ctatggctgc ctcttagacc atgtccggga aaaccgcgga cgcctgggct 2641 cccaggacct gctgaactgg tgtatgcaga ttgccaaggg gatgagctac ctggaggatg 2701 tgcggctcgt acacagggac ttggccgctc ggaacgtgct ggtcaagagt cccaaccatg 2761 tcaaaattac agacttcggg ctggctcggc tgctggacat tgacgagaca gagtaccatg 2821 cagatggggg caaggtgccc atcaagtgga tggcgctgga gtccattctc cgccggcggt 2881 tcacccacca gagtgatgtg tggagttatg gtgtgactgt gtgggagctg atgacttttg 2941 gggccaaacc ttacgatggg atcccagccc gggagatccc tgacctgctg gaaaaggggg 3001 agcggctgcc ccagcccccc atctgcacca ttgatgtcta catgatcatg gtcaaatgtt 3061 ggatgattga ctctgaatgt cggccaagat tccgggagtt ggtgtctgaa ttctcccgca 3121 tggccaggga cccccagcgc tttgtggtca tccagaatga ggacttgggc ccagccagtc 3181 ccttggacag caccttctac cgctcactgc tggaggacga tgacatgggg gacctggtgg 3241 atgctgagga gtatctggta ccccagcagg gcttcttctg tccagaccct gccccgggcg 3301 ctgggggcat ggtccaccac aggcaccgca gctcatctac caggagtggc ggtggggacc 3361 tgacactagg gctggagccc tctgaagagg aggcccccag gtctccactg gcaccctccg 3421 aaggggctgg ctccgatgta tttgatggtg acctgggaat gggggcagcc aaggggctgc 3481 aaagcctccc cacacatgac cccagccctc tacagcggta cagtgaggac cccacagtac 3541 ccctgccctc tgagactgat ggctacgttg cccccctgac ctgcagcccc cagcctgaat 3601 atgtgaacca gccagatgtt cggccccagc ccccttcgcc ccgagagggc cctctgcctg 3661 ctgcccgacc tgctggtgcc actctggaaa ggcccaagac tctctcccca gggaagaatg 3721 gggtcgtcaa agacgttttt gcctttgggg gtgccgtgga gaaccccgag tacttgacac 3781 cccagggagg agctgcccct cagccccacc ctcctcctgc cttcagccca gccttcgaca 3841 acctctatta ctgggaccag gacccaccag agcggggggc tccacccagc accttcaaag 3901 ggacacctac ggcagagaac ccagagtacc tgggtctgga cgtgccagtg tcaactttct 3961 tgtacaaagt tggcattata ggaaagcatt gcttatcaat ttgttgcaac gaacaggtca 4021 ctatcagtca aaataaaatc attatttgca tgcaagctta gatcctctag accagccagg 4081 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac 4141 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt 4201 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg 4261 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg 4321 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg 4381 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga 4441 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac 4501 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca 4561 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt 4621 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg 4681 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg 4741 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca 4801 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg 4861 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc 4921 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg 4981 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg 5041 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg 5101 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt 5161 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct 5221 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag 5281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 5341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 5401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 5461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 5521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 5581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 5641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 5701 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 5761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 5821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 5881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 5941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 6001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 6061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 6121 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 6181 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt 6241 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca 6301 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag 6361 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt 6421 tcgttttatt tgatgcctgg cagttcccta ctctcgcg //