LOCUS Exported 3347 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway ORF clone of human GRB2 [NM_002086.4] with stop codon (for native or N-terminal fusions). ACCESSION . VERSION . KEYWORDS R777-E099 Hs.GRB2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3347) TITLE Esposito lab REFERENCE 2 (bases 1 to 3347) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3347 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..45 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 37..54 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 38..54 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 84..183 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(838..937) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 1314..2105 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2198..2786 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2687..2706 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2940..2957 /label=L4440 /note="L4440 vector, forward primer" terminator 3116..3143 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3235..3321 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa 61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt 121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg 181 gcaccatgga agccatcgcc aaatatgact tcaaagctac tgcagacgac gagctgagct 241 tcaaaagggg ggacatcctc aaggttttga acgaagaatg tgatcagaac tggtacaagg 301 cagagcttaa tggaaaagac ggcttcattc ccaagaacta catagaaatg aaaccacatc 361 cgtggttttt tggcaaaatc cccagagcca aggcagaaga aatgcttagc aaacagcggc 421 acgatggggc ctttcttatc cgagagagtg agagcgctcc tggggacttc tccctctctg 481 tcaagtttgg aaacgatgtg cagcacttca aggtgctccg agatggagcc gggaagtact 541 tcctctgggt ggtgaagttc aattctttga atgagctggt ggattatcac agatctacat 601 ctgtctccag aaaccagcag atattcctgc gggacataga acaggtgcca cagcagccga 661 catacgtcca ggccctcttt gactttgatc cccaggagga tggagagctg ggcttccgcc 721 ggggagattt tatccatgtc atggataact cagaccccaa ctggtggaaa ggagcttgcc 781 acgggcagac cggcatgttt ccccgcaatt atgtcacccc cgtgaaccgg aacgtctaat 841 caactttctt gtacaaagtt ggcattatag gaaagcattg cttatcaatt tgttgcaacg 901 aacaggtcac tatcagtcaa aataaaatca ttatttgcat gcaagcttag atcctctaga 961 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac 1021 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt 1081 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg 1141 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta 1201 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 1261 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg 1321 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc 1381 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga 1441 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc 1501 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc 1561 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag 1621 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg 1681 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg 1741 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat 1801 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg 1861 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg 1921 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg 1981 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg 2041 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca 2101 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact 2161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 2221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 2281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 2341 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 2401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2581 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2821 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2881 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2941 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 3001 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 3061 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag 3121 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc 3181 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt 3241 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga 3301 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcg //