LOCUS       Exported                5828 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway ORF clone of human PIK3CD [NM_005026.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    R777-E167 Hs.PIK3CD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5828)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3319..3418)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3795..4586
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4679..5267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5168..5187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5421..5438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      5597..5624
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      5716..5802
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc ccctggggtg gactgcccca tggaattctg gaccaaggag gagaatcaga
      241 gcgttgtggt tgacttcctg ctgcccacag gggtctacct gaacttccct gtgtcccgca
      301 atgccaacct cagcaccatc aagcagctgc tgtggcaccg cgcccagtat gagccgctct
      361 tccacatgct cagtggcccc gaggcctatg tgttcacctg catcaaccag acagcggagc
      421 agcaagagct ggaggacgag caacggcgtc tgtgtgacgt gcagcccttc ctgcccgtcc
      481 tgcgcctggt ggcccgtgag ggcgaccgcg tgaagaagct catcaactca cagatcagcc
      541 tcctcatcgg caaaggcctc cacgagtttg actccttgtg cgacccagaa gtgaacgact
      601 ttcgcgccaa gatgtgccaa ttctgcgagg aggcggccgc ccgccggcag cagctgggct
      661 gggaggcctg gctgcagtac agtttccccc tgcagctgga gccctcggct caaacctggg
      721 ggcctggtac cctgcggctc ccgaaccggg cccttctggt caacgttaag tttgagggca
      781 gcgaggagag cttcaccttc caggtgtcca ccaaggacgt gccgctggcg ctgatggcct
      841 gtgccctgcg gaagaaggcc acagtgttcc ggcagccgct ggtggagcag ccggaagact
      901 acacgctgca ggtgaacggc aggcatgagt acctgtatgg cagctacccg ctctgccagt
      961 tccagtacat ctgcagctgc ctgcacagtg ggttgacccc tcacctgacc atggtccatt
     1021 cctcctccat cctcgccatg cgggatgagc agagcaaccc tgccccccag gtccagaaac
     1081 cgcgtgccaa accacctccc attcctgcga agaagccttc ctctgtgtcc ctgtggtccc
     1141 tggagcagcc gttccgcatc gagctcatcc agggcagcaa agtgaacgcc gacgagcgga
     1201 tgaagctggt ggtgcaggcc gggcttttcc acggcaacga gatgctgtgc aagacggtgt
     1261 ccagctcgga ggtgagcgtg tgctcggagc ccgtgtggaa gcagcggctg gagttcgaca
     1321 tcaacatctg cgacctgccc cgcatggccc gtctctgctt tgcgctgtac gccgtgatcg
     1381 agaaagccaa gaaggctcgc tccaccaaga agaagtccaa gaaggcggac tgccccattg
     1441 cctgggccaa cctcatgctg tttgactaca aggaccagct taagaccggg gaacgctgcc
     1501 tctacatgtg gccctccgtc ccagatgaga agggcgagct gctgaacccc acgggcactg
     1561 tgcgcagtaa ccccaacacg gatagcgccg ctgccctgct catctgcctg cccgaggtgg
     1621 ccccgcaccc cgtgtactac cccgccctgg agaagatctt ggagctgggg cgacacagcg
     1681 agtgtgtgca tgtcaccgag gaggagcagc tgcagctgcg ggaaatcctg gagcggcggg
     1741 ggtctgggga gctgtatgag cacgagaagg acctggtgtg gaagctgcgg catgaagtcc
     1801 aggagcactt cccggaggcg ctagcccggc tgctgctggt caccaagtgg aacaagcatg
     1861 aggatgtggc ccagatgctc tacctgctgt gctcctggcc ggagctgccc gtcctgagcg
     1921 ccctggagct gctagacttc agcttccccg attgccacgt aggctccttc gccatcaagt
     1981 cgctgcggaa actgacggac gatgagctgt tccagtacct gctgcagctg gtgcaggtgc
     2041 tcaagtacga gtcctacctg gactgcgagc tgaccaaatt cctgctggac cgggccctgg
     2101 ccaaccgcaa gatcggccac ttccttttct ggcacctccg ctccgagatg cacgtgccgt
     2161 cggtggccct gcgcttcggc ctcatcctgg aggcctactg caggggcagc acccaccaca
     2221 tgaaggtgct gatgaagcag ggggaagcac tgagcaaact gaaggccctg aatgacttcg
     2281 tcaagctgag ctctcagaag acccccaagc cccagaccaa ggagctgatg cacttgtgca
     2341 tgcggcagga ggcctaccta gaggccctct cccacctgca gtccccactc gaccccagca
     2401 ccctgctggc tgaagtctgc gtggagcagt gcaccttcat ggactccaag atgaagcccc
     2461 tgtggatcat gtacagcaac gaggaggcag gcagcggcgg cagcgtgggc atcatcttta
     2521 agaacgggga tgacctccgg caggacatgc tgaccctgca gatgatccag ctcatggacg
     2581 tcctgtggaa gcaggagggg ctggacctga ggatgacccc ctatggctgc ctccccaccg
     2641 gggaccgcac aggcctcatt gaggtggtac tccgttcaga caccatcgcc aacatccaac
     2701 tcaacaagag caacatggca gccacagccg ccttcaacaa ggatgccctg ctcaactggc
     2761 tgaagtccaa gaacccgggg gaggccctgg atcgagccat tgaggagttc accctctcct
     2821 gtgctggcta ttgtgtggcc acatatgtgc tgggcattgg cgatcggcac agcgacaaca
     2881 tcatgatccg agagagtggg cagctgttcc acattgattt tggccacttt ctggggaatt
     2941 tcaagaccaa gtttggaatc aaccgcgagc gtgtcccatt catcctcacc tacgactttg
     3001 tccatgtgat tcagcagggg aagactaata atagtgagaa atttgaacgg ttccggggct
     3061 actgtgaaag ggcctacacc atcctgcggc gccacgggct tctcttcctc cacctctttg
     3121 ccctgatgcg ggcggcaggc ctgcctgagc tcagctgctc caaagacatc cagtatctca
     3181 aggactccct ggcactgggg aaaacagagg aggaggcact gaagcacttc cgagtgaagt
     3241 ttaacgaagc cctccgtgag agctggaaaa ccaaagtgaa ctggctggcc cacaacgtgt
     3301 ccaaagacaa caggcagtaa tcaactttct tgtacaaagt tggcattata ggaaagcatt
     3361 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     3421 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     3481 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     3541 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     3601 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     3661 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     3721 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3781 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     3841 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     3901 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3961 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     4021 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     4081 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     4141 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     4201 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     4261 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     4321 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     4381 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     4441 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     4501 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     4561 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     4621 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4681 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4741 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4801 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4861 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4921 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4981 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5041 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     5101 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5161 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5221 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5281 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5341 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5401 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5461 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5521 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     5581 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     5641 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     5701 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     5761 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     5821 ctctcgcg
//
