LOCUS       Exported                3683 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway ORF clone of human PPP1CA [NM_002708.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    R777-E188 Hs.PPP1CA-nostop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3683)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1174..1273)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1650..2441
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2534..3122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3023..3042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3276..3293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      3452..3479
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3571..3657
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc cgacagcgag aagctcaacc tggactcgat catcgggcgc ctgctggaag
      241 tgcagggctc gcggcctggc aagaatgtac agctgacaga gaacgagatc cgcggtctgt
      301 gcctgaaatc ccgggagatt tttctgagcc agcccattct tctggagctg gaggcacccc
      361 tcaagatctg cggtgacata cacggccagt actacgacct tctgcgacta tttgagtatg
      421 gcggtttccc tcccgagagc aactacctct ttctggggga ctatgtggac aggggcaagc
      481 agtccttgga gaccatctgc ctgctgctgg cctataagat caagtacccc gagaacttct
      541 tcctgctccg tgggaaccac gagtgtgcca gcatcaaccg catctatggt ttctacgatg
      601 agtgcaagag acgctacaac atcaaactgt ggaaaacctt cactgactgc ttcaactgcc
      661 tgcccatcgc ggccatagtg gacgaaaaga tcttctgctg ccacggaggc ctgtccccgg
      721 acctgcagtc tatggagcag attcggcgga tcatgcggcc cacagatgtg cctgaccagg
      781 gcctgctgtg tgacctgctg tggtctgacc ctgacaagga cgtgcagggc tggggcgaga
      841 acgaccgtgg cgtctctttt acctttggag ccgaggtggt ggccaagttc ctccacaagc
      901 acgacttgga cctcatctgc cgagcacacc aggtggtaga agacggctac gagttctttg
      961 ccaagcggca gctggtgaca cttttctcag ctcccaacta ctgtggcgag tttgacaatg
     1021 ctggcgccat gatgagtgtg gacgagaccc tcatgtgctc tttccagatc ctcaagcccg
     1081 ccgacaagaa caaggggaag tacgggcagt tcagtggcct gaaccctgga ggccgaccca
     1141 tcaccccacc ccgcaattcc gccaaagcca agaaatcaac tttcttgtac aaagttggca
     1201 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     1261 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     1321 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     1381 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     1441 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     1501 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     1561 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     1621 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     1681 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1741 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1801 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1861 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1921 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1981 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2041 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2101 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2161 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2221 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2281 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     2341 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     2401 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     2461 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2521 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2581 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2641 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2701 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2761 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2821 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2881 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     2941 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3001 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3061 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     3421 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     3481 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     3541 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     3601 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     3661 cctggcagtt ccctactctc gcg
//
