LOCUS Exported 5024 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway ORF clone of human RASAL1 [NM_001193521.1] with stop codon (for native or N-terminal fusions). ACCESSION . VERSION . KEYWORDS R777-E233 Hs.RASAL1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5024) TITLE Esposito lab REFERENCE 2 (bases 1 to 5024) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5024 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..45 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 37..54 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 38..54 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 84..183 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2515..2614) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 2991..3782 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3875..4463 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4364..4383 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4617..4634 /label=L4440 /note="L4440 vector, forward primer" terminator 4793..4820 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4912..4998 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa 61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt 121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg 181 gcaccatggc caagagcagc tccctgaatg ttcgcgtggt ggagggccgc gcgctgcctg 241 ccaaggacgt gtctgggagc agcgacccct actgcctagt gaaagtggac gacgaggtgg 301 tggccaggac agctactgtc tggaggagcc tgggcccctt ctggggggag gagtacacgg 361 tgcacctgcc tctggatttc caccagctgg ccttctacgt gctggatgag gacactgtcg 421 ggcacgacga catcatcggc aagatctcgc tgagcaggga ggcgattaca gccgaccccc 481 gagggattga cagctggatt aacttgagcc gagtggaccc agatgcagaa gtgcagggtg 541 agatctgcct gtcagtgcag atgctggagg atgggcaggg ccgctgcctt cgctgccatg 601 tgcttcaggc cagggacctg gctcccagag acatctctgg cacatctgac ccatttgcac 661 gtgtgttttg gggcagccag agcttggaga cctcaaccat caagaagact cgcttcccgc 721 actgggatga agtgctggag ctgcgggaga tgccaggtgc cccgtcccca ctgcgggtgg 781 agctctggga ctgggacatg gtgggcaaga atgacttctt gggcatggtg gagttctctc 841 caaagaccct ccagcagaag ccacctaaag gctggttccg cctcctgccc tttcccagag 901 ccgaggagga ttctgggggg aacctgggtg ccctgcgagt gaaggtacgc ctgattgagg 961 accgcgtcct gccctcccag tgctaccagc ctctcatgga gctgctcatg gagtctgtgc 1021 aggggccagc agaggaggac actgctagcc ccttggcttt gctggaagag ctgaccttgg 1081 gggactgccg ccaggacctt gccaccaagc tggtgaaact ctttcttggc cggggactgg 1141 ctgggcactt tctggactat ctcacccggc gtgaggtggc tcggaccatg gaccccaaca 1201 ccctcttccg ttctaactcc ctggcatcca agtcgatgga acagtttatg aagctcgtgg 1261 gcatgcccta cctgcacgag gtcctgaagc ctgtgattag ccgtgtcttt gaggagaaga 1321 agtacatgga gctggatccc tgcaagatgg acctgggccg cacccggagg atctccttca 1381 aaggcgcact ctcggaggag cagatgcggg agaccagcct ggggctgctg acgggctacc 1441 tggggcccat cgtggacgcc atcgtgggct ccgtggggcg ctgcccgccc gccatgcgcc 1501 tcgccttcaa gcagctgcac cggcgagtgg aggagcgctt cccccaggcc gagcaccagg 1561 atgtgaagta cctggccatc agtggatttc tcttcttgcg attcttcgca cctgccatcc 1621 ttaccccaaa gctgtttgac cttcgggacc aacacgcgga cccccagact agccgctcac 1681 tgctgttgct tgccaaggct gtgcagagca ttggaaacct gggccagcag ctgggccaag 1741 gcaaggaact gtggatggcc cccctgcacc ccttcctgct gcagtgtgtc tcacgtgtga 1801 gagacttcct ggaccggctg gtggatgtgg atggggatga agctggtgtc ccagccaggg 1861 ccctgttccc gccctcggcc attgttcgag aaggctatct gctgaagcgc aaggaggagc 1921 ctgccggcct ggccacgcgc tttgccttca agaagcgcta cgtctggctc agcggggaga 1981 ccctctcctt ctccaagagt cctgagtggc aggtggtgac gcaggacggc acgggggcgc 2041 tgcacaccac ctacctccag tgcaagaatg tgaatgagct caaccagtgg ctctcggcct 2101 tgcgcaaggc cagcgccccc aacccgaaca agctggccgc ctgccacccc ggtgccttcc 2161 gcagcgcgcg ctggacctgc tgcctccagg ctgagcgctc agccgccggc tgcagccgta 2221 cacactcagc tgtcaccctg ggggactgga gtgacccact ggatcctgat gctgaggccc 2281 agacagtgta tcggcagctg ctcctggggc gggaccagct caggctgaaa ttactggagg 2341 attctaacat ggatacaact ctggaggcag acacaggggc ctgtcctgag gtcctggccc 2401 ggcaaagagc agcaactgcc cgcctgctgg aggtgctcgc agacctggat cgtgcccacg 2461 aggagttcca gcagcaggag cgagggaagg cggccctggg cccccttggc ccctaatcaa 2521 ctttcttgta caaagttggc attataggaa agcattgctt atcaatttgt tgcaacgaac 2581 aggtcactat cagtcaaaat aaaatcatta tttgcatgca agcttagatc ctctagacca 2641 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt 2701 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat 2761 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta 2821 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc 2881 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca 2941 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag 3001 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc 3061 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc 3121 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc 3181 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc 3241 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta 3301 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc 3361 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg 3421 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg 3481 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc 3541 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc 3601 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca 3661 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag 3721 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat 3781 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag 3841 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 3901 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 3961 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 4021 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 4081 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4141 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4201 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4261 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4321 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4381 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4441 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4501 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4561 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 4621 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 4681 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 4741 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc 4801 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc 4861 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct 4921 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg 4981 agcctttcgt tttatttgat gcctggcagt tccctactct cgcg //