LOCUS       Exported                4115 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway ORF clone of human SHC1 [NM_003029.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    R777-E308 Hs.SHC1-nostop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4115)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1606..1705)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2082..2873
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2966..3554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3455..3474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3708..3725
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      3884..3911
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      4003..4089
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa caagctgagt ggaggcggcg ggcgcaggac tcgggtggaa gggggccagc
      241 ttgggggcga ggagtggacc cgccacggga gctttgtcaa taagcccacg cggggctggc
      301 tgcatcccaa cgacaaagtc atgggacccg gggtttccta cttggttcgg tacatgggtt
      361 gtgtggaggt cctccagtca atgcgtgccc tggacttcaa cacccggact caggtcacca
      421 gggaggccat cagtctggtg tgtgaggctg tgccgggtgc taagggggcg acaaggagga
      481 gaaagccctg tagccgcccg ctcagctcta tcctggggag gagtaacctg aaatttgctg
      541 gaatgccaat cactctcacc gtctccacca gcagcctcaa cctcatggcc gcagactgca
      601 aacagatcat cgccaaccac cacatgcaat ctatctcatt tgcatccggc ggggatccgg
      661 acacagccga gtatgtcgcc tatgttgcca aagaccctgt gaatcagaga gcctgccaca
      721 ttctggagtg tcccgaaggg cttgcccagg atgtcatcag caccattggc caggccttcg
      781 agttgcgctt caaacaatac ctcaggaacc cacccaaact ggtcacccct catgacagga
      841 tggctggctt tgatggctca gcatgggatg aggaggagga agagccacct gaccatcagt
      901 actataatga cttcccgggg aaggaacccc ccttgggggg ggtggtagac atgaggcttc
      961 gggaaggagc cgctccaggg gctgctcgac ccactgcacc caatgcccag acccccagcc
     1021 acttgggagc tacattgcct gtaggacagc ctgttggggg agatccagaa gtccgcaaac
     1081 agatgccacc tccaccaccc tgtccagcag gcagagagct ttttgatgat ccctcctatg
     1141 tcaacgtcca gaacctagac aaggcccggc aagcagtggg tggtgctggg ccccccaatc
     1201 ctgctatcaa tggcagtgca ccccgggacc tgtttgacat gaagcccttc gaagatgctc
     1261 ttcgcgtgcc tccacctccc cagtcggtgt ccatggctga gcagctccga ggggagccct
     1321 ggttccatgg gaagctgagc cggcgggagg ctgaggcact gctgcagctc aatggggact
     1381 tcctggtacg ggagagcacg accacacctg gccagtatgt gctcactggc ttgcagagtg
     1441 ggcagcctaa gcatttgcta ctggtggacc ctgagggtgt ggttcggact aaggatcacc
     1501 gctttgaaag tgtcagtcac cttatcagct accacatgga caatcacttg cccatcatct
     1561 ctgcgggcag cgaactgtgt ctacagcaac ctgtggagcg gaaactgtca actttcttgt
     1621 acaaagttgg cattatagga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     1681 tcagtcaaaa taaaatcatt atttgcatgc aagcttagat cctctagacc agccaggaca
     1741 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1801 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1861 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1921 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1981 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2041 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2101 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2161 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2221 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2281 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2341 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2401 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2461 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2521 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2581 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2641 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2701 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2761 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2821 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2881 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     3361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3601 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3661 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3721 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3781 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3841 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3901 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3961 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4021 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4081 ttttatttga tgcctggcag ttccctactc tcgcg
//
