LOCUS Exported 10859 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR. ACCESSION . VERSION . KEYWORDS pMM705 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10859) AUTHORS Mimee M, Tucker AC, Voigt CA, Lu TK TITLE Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. JOURNAL Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001. PUBMED 26918244 REFERENCE 2 (bases 1 to 10859) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10859 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 28..4134 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" misc_RNA 4294..4369 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS 4815..5897 /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind complement(4834..4853) /label=LacI-R /note="LacI, reverse primer" CDS 9060..9920 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9278..9297) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" oriT 10051..10160 /note="incP origin of transfer" rep_origin 10331..10719 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" protein_bind 10793..10809 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 10826..10842 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 cgtcaaacaa atataaataa tataaacatg gataagaaat actcaatagg cttagctatc 61 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag 121 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt 181 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg 241 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg 301 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag 361 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa 421 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg 481 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag 541 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc 601 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt 661 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt 721 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat 781 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac 841 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg 901 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa 961 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac 1021 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt 1081 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa 1141 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg 1201 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc 1261 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca 1321 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat 1381 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa 1441 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt 1501 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat 1561 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact 1621 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta 1681 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa 1741 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta 1801 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa 1861 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg 1921 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt 1981 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg 2041 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc 2101 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca 2161 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct 2221 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg 2281 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa 2341 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta 2401 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc 2461 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat 2521 cgtttaagtg attatgatgt cgatgccatt gttccacaaa gtttccttaa agacgattca 2581 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca 2641 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta 2701 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt 2761 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg 2821 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga 2881 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa 2941 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc 3001 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt 3061 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa 3121 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca 3181 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa 3241 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa 3301 gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatttta 3361 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat 3421 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa 3481 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga 3541 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa 3601 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa 3661 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa 3721 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat 3781 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag 3841 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt 3901 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat 3961 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt 4021 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc 4081 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga ctgaattaat 4141 gcggctgcaa tttttttggg cggggccgcc caaaaaaatc ctagcaccct gcagcagtac 4201 tgcttgacca taagaacaaa aaaacttccg ataaagtttg gaagataaag ctaaaagttc 4261 ttatctttgc agtgaaaatg gggtgtatcc tgcgttttag agctagaaat agcaagttaa 4321 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctag 4381 atgatgatta aggatcttgc gtacaatttg acgatcgttt cacgctaaga tgctggctaa 4441 ttgcctatct tccagtgatg gaacagcatt tgtgcattgg ctgcaacaat cagccttgat 4501 ctggaagaag caatgaaagc tgctgttaag tctccgaatc aggtattgtt cctgacaggt 4561 gtattcccat ccggtaaacg cggatacttt gcagttgatc tgactcagga ataaattata 4621 aattaaggta agaagattgt aggataagct aatgaaatag aaaaaggatg ccgtcacaca 4681 acttgtcggc attctttttt gttttattag ttgaaaatat agtgaaaaag ttgcctaaat 4741 atgtatgtta acaaattatt tgtcgtaact ttgcactcca aatctgtttt taacatatgg 4801 cactagtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 4861 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 4921 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 4981 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 5041 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 5101 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 5161 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 5221 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 5281 tcaacagtat tattttctcc catgaggacg gtacgcgact gggcgtggag catctggtcg 5341 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 5401 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 5461 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 5521 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 5581 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata 5641 ccgaggacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 5701 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 5761 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 5821 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 5881 tggaaagcgg gcagtgagct ttcctcggta ccaaattcca gaaaagaggc ctcccgaaag 5941 gggggccttt tttcgttttg gtcctacttg tgcctgttct atttccgaac cgaccgcttg 6001 tatgaatcca tcaaaattcg ttttctctat gttggattcc ttgttgctca tattgtgatg 6061 ataatttcta caaatatagt cattggtaac tatctatgaa actgtttgat acttttatag 6121 ttgattaaac ttgttcatgg catttgcctt aatatcatcc gctatgtcaa tgtagggttt 6181 catagctttg tagtcgctgt gtcccgtcca tttcatgacc acctgtgccg ggattccgag 6241 agccagcgca ttgcagatga atgtccttct tcctgcatgg gtactgagca aagcgtattt 6301 gggtgtgact tcatcaatac gttcatttcc cttgtagtag gtttcccgta caggctcgtt 6361 gatttctgcc agttcgccca gctctttcag gtaatcgttc atcttctggt tgctgatgac 6421 gggcagagcc atgtaattct cgaaatggat gtccttgtat ttgtccagta tggctttgct 6481 gtatttgttc agttcaatcg tcaggctgtc ggcagtcttg actgtggtta tttcgatgtg 6541 gtcggacttc acatcgcttc ttttcagatt gcgaacatcc gaataccgca aactcgtaaa 6601 gcagcagaac aggaaaacat cacgcacacg ttccaggtat tgcttatcct tgggtatctg 6661 gtagtctttc agcttgttca gttcatccca agtcaggaag attacttttt tcgaggtggt 6721 tttcagtttc ggtttgaacg tatcgtatgc aatgttctga tgatgtcctt tcttgaagct 6781 ccagcgcagg aaccatttga ggaatcccat ttgcttgccg atggtgctgt ttctcatatc 6841 cttggtgtca cgcaggaagt tgacgtattc gttcaatcca aactcgttga aatagttgaa 6901 cgttgcatcc tccttgaact ctttgaggtg gttcctcact gctgcaaatt tttcataggt 6961 ggatgccgtc cagttattct ggttaccgca ctcttttaca aactcatcga acacctccca 7021 aaagctgaca ggggcttctt ccggctgttc ttcgctggtg tctttcattc tcatgttgaa 7081 agcttccttc aactgttggg tcgttggcat gacctcctgc acctcaaatt ccttgaaaat 7141 attctggatt tcggcatagt atttcagcaa gtccgtattg atttcggctg cactttgctt 7201 tagcttgttg gtacatccgc tctttacccg ctgcttatct gcatcccatt tggctacgtc 7261 aatccggtag cccgttgtaa actcgatgcg ttggctggca aagatgacac gcatacggat 7321 gggtacgttc tctacgattg gcacaccgtt ctttttccgg ctctccaatg caaaaatgat 7381 gttgcgcttg atattcataa ttgggtgcgt ttgaaattct acacccaaat atacacccaa 7441 ttattgagat agcaaaagac atttagaaac atttactttt actctatatt gtaatttaca 7501 cttgattatc agtcgtttgc agtcttatga tattctgtga aagtataagt tcgagagcct 7561 gtctctccgc aaaaaacgct gaaaatcagc agattgcaaa acaaacaccc tgttttacac 7621 ccaagaatgt aaagtcggct gtttttgttt tatttaagat aatacaacca ctacataata 7681 aaagagtagc gatattaaaa gaatccgatg agaaaagact aatatttatc tatccattca 7741 gtttgatttt tcaggacttt acatcgtcct gaaagtattt gttggtaccg gtaccgagga 7801 cgcgtaaaca tttacagttg catgtggcct attgttttta gccgttaaat attttataac 7861 tattaaatag cgatacaaat tgttcgaaac taatattgtt tatatcatat attctcgcat 7921 gttttaaagc tttattaaat tgattttttg taaacagttt ttcgtactct ttgttaaccc 7981 atttcattac aaaagtttca tatttttttc tctctttaaa tgccattttt gctggctttc 8041 tttttaatac aattaatgtg ctatccactt taggttttgg atggaaataa tacctaggaa 8101 tttttgctaa tatagaaata tctacctctg ccattaacag caatgctagt gatctgtttg 8161 tatctaataa cattttagca aaaccatatt ccactattaa ataacttatt gtggctgaac 8221 tttcaaaaac aatttttcga attatatttg tgcttatgtt gtaaggtatg ctgccaaata 8281 ttttatatgg attgtggcta ggaaatgtaa atttcagtat atcatcattt actatttgat 8341 agttaggata atttaagagc ttattacgag ttacctcaca taatttagaa tcaatttcta 8401 tcgccgttac aaaattacat ctctttacca atccagcagt aaaatgacct ttccctgcac 8461 ctatttcaaa gatgttatct ttttcatcta aacttatgca attcattatt ttttctatgt 8521 gatattttga agtaataaaa ttttgactat cttttatatt tactttgttc attataacct 8581 ctccttaatt tattgcatct cttttcgaat atttatgttt tttgagaaaa gaacgtactc 8641 atggttcatc ccgatatgcg tatcggtctg tatatcagca actttctatg tgtttcaact 8701 acaatagtca tctattctca tctttctgag tccaccccct gcaaagcccc tctttacgac 8761 ataaaaattc ggtcggaaaa ggtatgcaaa agatgtttct ctctttaaga gaaactcttc 8821 gggatgcaaa aatatgaaaa taactccaat tcaccaaatt atatagcgac ttttttacaa 8881 aatgctaaaa tttgttgatt tccgtcaagc aattgttgag caaaaatgtc ttttacgata 8941 aaatgatacc tcaatatcaa ctgtttagca aaacgatatt tctcttaaag agagaaacac 9001 ctttttgttc accaatcccc gacttttaat cccgcggcca tgattgaaaa aggaagagta 9061 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 9121 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 9181 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 9241 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 9301 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 9361 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 9421 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 9481 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 9541 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 9601 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 9661 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 9721 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 9781 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 9841 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 9901 cactgattaa gcattggtaa ctgtcagacc aagtttactc ataacgcgtc aattcgaggg 9961 ggatcaattc cgtgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc 10021 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt 10081 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc 10141 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa 10201 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta 10261 taatgacccc gaagcagggt tatgcagcgg aaaacggaat tgatccggcc acgatgcgtc 10321 cggcgtagag gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca 10381 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa 10441 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt 10501 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta 10561 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt 10621 aacggttgtg gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa 10681 agcaattttg agtgacacag gaacacttaa cggctgacat gggaattccc ctccaccgcg 10741 gtggtacaaa gaaaattcga caaactgtta tttttctatc tatttatttg aattgtgagc 10801 ggataacaat tacctttgtc ggcaattgtg agcggataac aattaaataa agatattct //