LOCUS       Exported                 732 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pMM725
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 ttgagtgaca caggaacact taacggctga catgggaatt cccctccacc gcggtgggtt
       61 ccatcactgg aagataggca attagtagaa gcaatttgat tagtaatcta aaagcactcc
      121 gataacgatt agagtgcttt tagattgttt atcattaatt atcaaagcaa gtgcagttca
      181 ttatgccagg atgcgctcgc acagacgcca acccgtgaca ccgttgatgg tcacgcggaa
      241 cagcaggctg ccgtccgggt taatcagacg ctcgtcaata atcttattgc cattccacag
      301 ggtaccggtg acggtgatct ttttaccgtc aaaaacggca atgccttcat acggacggcc
      361 gaaatagtca atcatattcg gggtcacacc atcaatcacc agcgtaccgt aatgcaagat
      421 aaccttgaag tggtgatcgt caactgggta gacgacttta aagattttct cgatttgacc
      481 catttggtca ccgctcaggc cttcgtacgg gatgatcaca tgaatatcaa ttttcagacc
      541 gttctcgccg gacagaacga tgcgctgaat cggcgtcacg ctcacaccca ggttctggaa
      601 cagagagctt acgccaccct gttccaggac ttgatccaaa ttataacccg cggtctgacg
      661 ccaatcgcca acaaaatctt ccagagtaaa aaccatttta ttcttttacc ttaattccgc
      721 aacactatta ta
//
