LOCUS       Exported                 773 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pMM597
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    518..534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    551..567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgatggtca cgcggaacag caggctgccg tccgggttaa tcagacgctc gtcaataatc
       61 ttattgccat tccacagggt accggtgacg gtgatctttt taccgtcaaa aacggcaatg
      121 ccttcatacg gacggccgaa atagtcaatc atattcgggg tcacaccatc aatcaccagc
      181 gtaccgtaat gcaagataac cttgaagtgg tgatcgtcaa ctgggtagac gactttaaag
      241 attttctcga tttgacccat ttggtcaccg ctcaggcctt cgtacgggat gatcacatga
      301 atatcaattt tcagaccgtt ctcgccggac agaacgatgc gctgaatcgg cgtcacgctc
      361 acacccaggt tctggaacag agagcttacg ccaccctgtt ccaggacttg atccaaatta
      421 taacccgcgg tctgacgcca atcgccaaca aaatcttcca gagtaaaaac catgtttata
      481 ttatttatat ttgtttgacg agaatatctt tatttaattg ttatccgctc acaattgccg
      541 acaaaggtaa ttgttatccg ctcacaattc aaataaatag atagaaaaat aacagtttgt
      601 cgaattttct ttgtaccacc gcggtggagg ggaattccca tgtcagccgt taagtgttcc
      661 tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg
      721 cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat tct
//
