LOCUS       Exported                 742 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pMM725
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 aaacgaacct gggtgtgagc gtgaactgca aagataagaa cttttagctt tatcttccaa
       61 actttatcgg aagttttttt gttcttatgg tcaagcagta ctgctgcagg gtgctaggat
      121 ttttttgggc ggccccgccc aaaaaaattg cagccgcatt aattcagtca cctcctagct
      181 gactcaaatc aatgcgtgtt tcataaagac cagtgatgga ttgatggata agagtggcat
      241 ctaaaacttc ttttgtagac gtatatcgtt tacgatcaat tgttgtatca aaatatttaa
      301 aagcagcggg agctccaaga ttcgtcaacg taaataaatg aataatattt tctgcttgtt
      361 cacgtattgg tttgtctcta tgtttgttat atgcactaag aactttatct aaattggcat
      421 ctgctaaaat aacacgctta gaaaattcac tgatttgctc aataatctca tctaaataat
      481 gcttatgctg ctccacaaac aattgttttt gttcgttatc ttctggacta cccttcaact
      541 tttcataatg actagctaaa tataaaaaat tcacatattt gcttggcaga gccagctcat
      601 ttcctttttg taattctccg gcactagcca gcatccgttt acgaccgttt tctaactcaa
      661 aaagactata tttaggtagt ttaatgatta agtctttttt aacttcctta tatcctttag
      721 cttctaaaaa gtcaatcgga tt
//
