LOCUS       Exported                 804 bp ds-DNA     linear   SYN 16-JUN-2021
DEFINITION  IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pMM750
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 agctctaaaa ccgtgctaag gagtgagctt cactgcaaag ataagaactt ttagctttat
       61 cttccaaact ttatcggaag tttttttgtt cttatggtca agcagtactg ctgcagggtg
      121 ctaggatttt tttgggcggc cccgcccaaa aaaattgcag ccgcattaat tcagtcacct
      181 cctagctgac tcaaatcaat gcgtgtttca taaagaccag tgatggattg atggataaga
      241 gtggcatcta aaacttcttt tgtagacgta tatcgtttac gatcaattgt tgtatcaaaa
      301 tatttaaaag cagcgggagc tccaagattc gtcaacgtaa ataaatgaat aatattttct
      361 gcttgttcac gtattggttt gtctctatgt ttgttatatg cactaagaac tttatctaaa
      421 ttggcatctg ctaaaataac acgcttagaa aattcactga tttgctcaat aatctcatct
      481 aaataatgct tatgctgctc cacaaacaat tgtttttgtt cgttatcttc tggactaccc
      541 ttcaactttt cataatgact agctaaatat aaaaaattca catatttgct tggcagagcc
      601 agctcatttc ctttttgtaa ttctccggca ctagccagca tccgtttacg accgttttct
      661 aactcaaaaa gactatattt aggtagttta atgattaagt cttttttaac ttccttatat
      721 cctttagctt ctaaaaagtc aatcggattt ttttcaaagg aacttctttc cataattgtg
      781 atccctagta actctttaac ggat
//
