LOCUS       Exported                 804 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pMM763
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tagctctaaa actgcctgat gaggttttgt ttactgcaaa gataagaact tttagcttta
       61 tcttccaaac tttatcggaa gtttttttgt tcttatggtc aagcagtact gctgcagggt
      121 gctaggattt ttttgggcgg ccccgcccaa aaaaattgca gccgcattaa ttcagtcacc
      181 tcctagctga ctcaaatcaa tgcgtgtttc ataaagacca gtgatggatt gatggataag
      241 agtggcatct aaaacttctt ttgtagacgt atatcgttta cgatcaattg ttgtatcaaa
      301 atatttaaaa gcagcgggag ctccaagatt cgtcaacgta aataaatgaa taatattttc
      361 tgcttgttca cgtattggtt tgtctctatg tttgttatat gcactaagaa ctttatctaa
      421 attggcatct gctaaaataa cacgcttaga aaattcactg atttgctcaa taatctcatc
      481 taaataatgc ttatgctgct ccacaaacaa ttgtttttgt tcgttatctt ctggactacc
      541 cttcaacttt tcataatgac tagctaaata taaaaaattc acatatttgc ttggcagagc
      601 cagctcattt cctttttgta attctccggc actagccagc atccgtttac gaccgttttc
      661 taactcaaaa agactatatt taggtagttt aatgattaag tcttttttaa cttccttata
      721 tcctttagct tctaaaaagt caatcggatt tttttcaaag gaacttcttt ccataattgt
      781 gatccctagt aactctttaa cgga
//
