LOCUS       Exported                5572 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  NanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    pMM555
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3767..4627
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3985..4004)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4758..4867
                     /note="incP origin of transfer"
     rep_origin      5038..5426
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 atggttttta ctctggaaga ttttgttggc gattggcgtc agaccgcggg ttataatttg
       61 gatcaagtcc tggaacaggg tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg
      121 acgccgattc agcgcatcgt tctgtccggc gagaacggtc tgaaaattga tattcatgtg
      181 atcatcccgt acgaaggcct gagcggtgac caaatgggtc aaatcgagaa aatctttaaa
      241 gtcgtctacc cagttgacga tcaccacttc aaggttatct tgcattacgg tacgctggtg
      301 attgatggtg tgaccccgaa tatgattgac tatttcggcc gtccgtatga aggcattgcc
      361 gtttttgacg gtaaaaagat caccgtcacc ggtaccctgt ggaatggcaa taagattatt
      421 gacgagcgtc tgattaaccc ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc
      481 acgggttggc gtctgtgcga gcgcatcctg gcataatgaa ctgcacttgc tttgataatt
      541 aatgataaac aatctaaaag cactctaatc gttatcggag tgcttttaga ttactaatca
      601 aattgcttct actaattgcc tatcttccag tgatggaaca gcatttgtgc attggctgca
      661 acaatcagcc ttacttgtgc ctgttctatt tccgaaccga ccgcttgtat gaatccatca
      721 aaattcgttt tctctatgtt ggattccttg ttgctcatat tgtgatgata atttctacaa
      781 atatagtcat tggtaactat ctatgaaact gtttgatact tttatagttg attaaacttg
      841 ttcatggcat ttgccttaat atcatccgct atgtcaatgt agggtttcat agctttgtag
      901 tcgctgtgtc ccgtccattt catgaccacc tgtgccggga ttccgagagc cagcgcattg
      961 cagatgaatg tccttcttcc tgcatgggta ctgagcaaag cgtatttggg tgtgacttca
     1021 tcaatacgtt catttccctt gtagtaggtt tcccgtacag gctcgttgat ttctgccagt
     1081 tcgcccagct ctttcaggta atcgttcatc ttctggttgc tgatgacggg cagagccatg
     1141 taattctcga aatggatgtc cttgtatttg tccagtatgg ctttgctgta tttgttcagt
     1201 tcaatcgtca ggctgtcggc agtcttgact gtggttattt cgatgtggtc ggacttcaca
     1261 tcgcttcttt tcagattgcg aacatccgaa taccgcaaac tcgtaaagca gcagaacagg
     1321 aaaacatcac gcacacgttc caggtattgc ttatccttgg gtatctggta gtctttcagc
     1381 ttgttcagtt catcccaagt caggaagatt acttttttcg aggtggtttt cagtttcggt
     1441 ttgaacgtat cgtatgcaat gttctgatga tgtcctttct tgaagctcca gcgcaggaac
     1501 catttgagga atcccatttg cttgccgatg gtgctgtttc tcatatcctt ggtgtcacgc
     1561 aggaagttga cgtattcgtt caatccaaac tcgttgaaat agttgaacgt tgcatcctcc
     1621 ttgaactctt tgaggtggtt cctcactgct gcaaattttt cataggtgga tgccgtccag
     1681 ttattctggt taccgcactc ttttacaaac tcatcgaaca cctcccaaaa gctgacaggg
     1741 gcttcttccg gctgttcttc gctggtgtct ttcattctca tgttgaaagc ttccttcaac
     1801 tgttgggtcg ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt ctggatttcg
     1861 gcatagtatt tcagcaagtc cgtattgatt tcggctgcac tttgctttag cttgttggta
     1921 catccgctct ttacccgctg cttatctgca tcccatttgg ctacgtcaat ccggtagccc
     1981 gttgtaaact cgatgcgttg gctggcaaag atgacacgca tacggatggg tacgttctct
     2041 acgattggca caccgttctt tttccggctc tccaatgcaa aaatgatgtt gcgcttgata
     2101 ttcataattg ggtgcgtttg aaattctaca cccaaatata cacccaatta ttgagatagc
     2161 aaaagacatt tagaaacatt tacttttact ctatattgta atttacactt gattatcagt
     2221 cgtttgcagt cttatgatat tctgtgaaag tataagttcg agagcctgtc tctccgcaaa
     2281 aaacgctgaa aatcagcaga ttgcaaaaca aacaccctgt tttacaccca agaatgtaaa
     2341 gtcggctgtt tttgttttat ttaagataat acaaccacta cataataaaa gagtagcgat
     2401 attaaaagaa tccgatgaga aaagactaat atttatctat ccattcagtt tgatttttca
     2461 ggactttaca tcgtcctgaa agtatttgtt ggtaccggta ccgaggacgc gtaaacattt
     2521 acagttgcat gtggcctatt gtttttagcc gttaaatatt ttataactat taaatagcga
     2581 tacaaattgt tcgaaactaa tattgtttat atcatatatt ctcgcatgtt ttaaagcttt
     2641 attaaattga ttttttgtaa acagtttttc gtactctttg ttaacccatt tcattacaaa
     2701 agtttcatat ttttttctct ctttaaatgc catttttgct ggctttcttt ttaatacaat
     2761 taatgtgcta tccactttag gttttggatg gaaataatac ctaggaattt ttgctaatat
     2821 agaaatatct acctctgcca ttaacagcaa tgctagtgat ctgtttgtat ctaataacat
     2881 tttagcaaaa ccatattcca ctattaaata acttattgtg gctgaacttt caaaaacaat
     2941 ttttcgaatt atatttgtgc ttatgttgta aggtatgctg ccaaatattt tatatggatt
     3001 gtggctagga aatgtaaatt tcagtatatc atcatttact atttgatagt taggataatt
     3061 taagagctta ttacgagtta cctcacataa tttagaatca atttctatcg ccgttacaaa
     3121 attacatctc tttaccaatc cagcagtaaa atgacctttc cctgcaccta tttcaaagat
     3181 gttatctttt tcatctaaac ttatgcaatt cattattttt tctatgtgat attttgaagt
     3241 aataaaattt tgactatctt ttatatttac tttgttcatt ataacctctc cttaatttat
     3301 tgcatctctt ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg gttcatcccg
     3361 atatgcgtat cggtctgtat atcagcaact ttctatgtgt ttcaactaca atagtcatct
     3421 attctcatct ttctgagtcc accccctgca aagcccctct ttacgacata aaaattcggt
     3481 cggaaaaggt atgcaaaaga tgtttctctc tttaagagaa actcttcggg atgcaaaaat
     3541 atgaaaataa ctccaattca ccaaattata tagcgacttt tttacaaaat gctaaaattt
     3601 gttgatttcc gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa tgatacctca
     3661 atatcaactg tttagcaaaa cgatatttct cttaaagaga gaaacacctt tttgttcacc
     3721 aatccccgac ttttaatccc gcggccatga ttgaaaaagg aagagtatga gtattcaaca
     3781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4621 ttggtaactg tcagaccaag tttactcata acgcgtcaat tcgaggggga tcaattccgt
     4681 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
     4741 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     4801 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     4861 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     4921 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     4981 gcagggttat gcagcggaaa acggaattga tccggccacg atgcgtccgg cgtagaggat
     5041 ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
     5101 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
     5161 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
     5221 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
     5281 tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac
     5341 aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt
     5401 gacacaggaa cacttaacgg ctgacatggg aattcccctc caccgcggtg gtacaaagaa
     5461 aattcgacaa actgttattt ttctatctat ttatttgggt gggaaacttt agttatgtac
     5521 ctttgtcggc aaataaagat attctcgtca aacaaatata aataatataa ac
//
