LOCUS       Exported                3522 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    pDGB1_omega2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     125..144
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     338..360
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     352..369
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     353..369
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    370..426
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(439..455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(439..455)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(452..474)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    463..479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(487..517)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    532..553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    632..656
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(747..1335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(827..846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1506..2297)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2941..3376
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3503..3
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 taacgatatc ggtctcagtc aggagtgaga cgcacagctt gtctgtaagc ggatgccggg
       61 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
      121 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
      181 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
      241 tgggaagggc gatcggcgca ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      301 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      361 acggccagtg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
      421 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      481 tccacacaac atacgagccg gaagcatata gtgtaaagcc tggggtgcct aatgagtgag
      541 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      601 ccagctgcgt ctcacgcttg agaccgatat ctgacaggat atattggcgg gtaaactaag
      661 tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa ggccaggaac
      721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1201 atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc ttgatccggc
     1261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtgta acattggtct
     1501 agtgattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     1561 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     1621 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     1681 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     1741 acatttcgct catcaccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     1801 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     1861 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     1921 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     1981 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     2041 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     2101 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     2161 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     2221 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     2281 atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
     2341 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
     2401 gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact
     2461 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     2521 tacttgcatt acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc
     2581 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg
     2641 tcctggctgg aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     2701 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     2761 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     2821 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     2881 ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcgatcc
     2941 ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt ggatagaggg
     3001 tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg gctttccggc
     3061 ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga
     3121 gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg
     3181 ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa gtagttacag
     3241 caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta ttggtcgccc
     3301 ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt
     3361 gcccaccgtc gagcgccttt gcccacaacc cggcggccgg ccgcaacaga tcgttttata
     3421 aatttttttt tttgaaaaag aaaaagcccg aaaggcggca acctctcggg cttctggatt
     3481 tccgatcccc ggaattagat cttggcagga tatattgtgg tg
//
