LOCUS Exported 1003 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS mCherry-hLC3B-pcDNA3.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1003) AUTHORS Jahreiss L, Menzies FM, Rubinsztein DC TITLE The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes. JOURNAL Traffic. 2008 Apr;9(4):574-87. Epub 2008 Jan 7. PUBMED 18182013 REFERENCE 2 (bases 1 to 1003) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1003 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 36..55 /label=T7 /note="T7 promoter, forward primer" promoter 36..54 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 75..84 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 81..788 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(226..244) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 494..513 /label=mCherry-F /note="mCherry, forward primer" ORIGIN 1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc 61 caagcttggt acccgccacc atggtgagca agggcgagga ggataacatg gccatcatca 121 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga 181 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga 241 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct 301 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg 361 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc 421 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact 481 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc 541 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg 601 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc 661 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca 721 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc 781 tgtacaagca aggatccatg ccgtcggaga agaccttcaa gcagcgccgc accttcgaac 841 aaagagtaga agatgtccga cttattcgag agcagcatcc aaccaaaatc ccggtgataa 901 tagaacgata caagggtgag aagcagcttc ctgttctgga taaaacaaag ttccttgtac 961 ctgaccatgt caacatgagt gagctcatca agataattag aag //