LOCUS       Exported                 912 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  Transcriptional unit for kanamycin resistance gene plant expression 
            .
ACCESSION   .
VERSION     .
KEYWORDS    pEGB Tnos:NptII:Pnos (GB0226)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    47..69
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      196..631
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tagatcgacc ggcatgcaag ctgatcgctc tcctgagacg gatccgttac accacaatat
       61 atcctgccaa gatctaattc cggggatcgg aaatccagaa gcccgagagg ttgccgcctt
      121 tcgggctttt tctttttcaa aaaaaaaaat ttataaaacg atctgttgcg gccggccgcc
      181 gggttgtggg caaaggcgct cgacggtggg caaccgcttg cggttgtcca cgggcggagc
      241 cggtgcgcgt agcgcattgt ccacaagcca agggcgacca ataattgata tatatattca
      301 taattgaaaa gctaattgaa catactactt gctgtaacta cttgccggag cgaggggtgt
      361 ttgcaagctg ttgatctgaa agggctatta gcgttctcac gtgccttttt gattagcgat
      421 ttcacgtgac cttattagcg atttcacgta ctccgattag cgatttcacg taccctgatt
      481 agcgatttca cgtggatagt ttttggagcg ggccggaaag ccccgtgaat caaggctttg
      541 cggggcatta gcggtttcac gtggataact accctctatc cacaggcttc cggggataaa
      601 aaagcccgct cgacggcggg ctgttggatg gggatcgcct gaatcgcccc atcatccagc
      661 cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt
      721 ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc
      781 ttcaactcag caaaagttcg atttattcaa caaagccacg ttgtgtctca aaatctctga
      841 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
      901 aacagtaaat ac
//
