LOCUS Exported 4004 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Transcriptional unit for kanamycin resistance gene plant expression . ACCESSION . VERSION . KEYWORDS pEGB Tnos:NptII:Pnos (GB0226) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4004) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 4004) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4004 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 24..276 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(316..1110) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(427..446) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 1037..1056 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter complement(1144..1323) /label=NOS promoter /note="nopaline synthase promoter" misc_feature 1423..1447 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(1538..2126) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1618..1637) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2297..3112) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 3020..3039 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 3104..3123 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 3403..3838 /label=pSa ori /note="origin of replication from bacterial plasmid pSa" misc_feature 3965..3987 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" ORIGIN 1 agcgatcagc ttgcatgccg gtcgatctag taacatagat gacaccgcgc gcgataattt 61 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac 121 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg 181 cttaacgtaa ttcaacagaa attatatgat aatcatggca agaccggcaa caggattcaa 241 tcttaagaaa ctttattgcc aaatgtttga acgatctgct tgactctagc tagagtccga 301 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcta tgcgctgcga 361 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc 421 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg 481 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc 541 gtcgccgtgg gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga gcctggcgaa 601 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc 661 ggcttccatc cgagtacgtg ctcgctcgat gcggtgtttc gcttggtggt cgaatgggca 721 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc 781 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca 841 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc 901 cagccacgat agccgcgctg cctcgtcttg gagttcattc agggcaccgg acaggtcggt 961 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca 1021 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga 1081 acctgcgtgc aatccatctt gttcaatcat gcctcgatcg agttgagagt gaatatgaga 1141 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat 1201 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt 1261 atgtgcttag ctcattaaac tccagaaacc cgcggctcag tggctccttc aacgttgcgg 1321 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataaccgt 1381 gactccctta attctcatgt atctccgtca tgagacggat cctgacagga tatattggcg 1441 ggtaaactaa gtcgctgtat gtgtttgttt gagatctcat gtgagcaaaa ggccagcaaa 1501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 1561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 1621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 1681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 1741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 1801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 1861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 1921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 1981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaagaaga gttggtagct 2041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 2101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 2161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 2221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgtgt 2281 aacattggtc tagtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca 2341 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact 2401 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc 2461 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat 2521 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga 2581 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt 2641 tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat 2701 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt 2761 cacctgaatc aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg 2821 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa 2881 attccgtcag ccagtttagt ctgaccatct catctgtaac aacattggca acgctacctt 2941 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg 3001 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt 3061 tggaatttaa tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacacccc 3121 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt 3181 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt 3241 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa 3301 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct 3361 ccctcacttt ctggctggat gatggggcga ttcaggcgat ccccatccaa cagcccgccg 3421 tcgagcgggc ttttttatcc ccggaagcct gtggatagag ggtagttatc cacgtgaaac 3481 cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg gcccgctcca aaaactatcc 3541 acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg gagtacgtga aatcgctaat 3601 aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa cgctaatagc cctttcagat 3661 caacagcttg caaacacccc tcgctccggc aagtagttac agcaagtagt atgttcaatt 3721 agcttttcaa ttatgaatat atatatcaat tattggtcgc ccttggcttg tggacaatgc 3781 gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg tcgagcgcct 3841 ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta taaatttttt tttttgaaaa 3901 agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga tttccgatcc ccggaattag 3961 atcttggcag gatatattgt ggtgtaacgg atccgtctca ggag //