LOCUS       Exported                 802 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCoPURO
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Iwaki T, Figuera M, Ploplis VA, Castellino FJ
  TITLE     Rapid selection of Drosophila S2 cells with the puromycin resistance
            gene.
  JOURNAL   Biotechniques. 2003 Sep . 35(3):482-4, 486.
  PUBMED    14513552
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(598..617)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag
      121 gtcgactcta gaggatccgg tgcgtggtgg tttcatgctt ctgggaacgg caaatgggtt
      181 taggattggg aacccctcat catctgttgg aatatactat tcaacctaca aaagtaacgt
      241 taaacaacac tactttatat ttgatatgaa tggccacacc ttttatgcca taaaacatat
      301 tgtaagagaa taccactctt tttattcctt ctttccttct tgtacgtttt ttgctgtaag
      361 taggtcgtgg tgctggtgtt gcagttgaaa taacttaaaa tataaatcat aaaactcaaa
      421 cataaacttg actatttatt tatttattaa gaaaggaaat ataaattata aattacaaca
      481 gatcccccgg gctgcaggaa ttcgatagct tgcatgcctg caggtcggcc gccacgaccg
      541 gtgccgccac catcccctga cccacgcccc tgacccctca caaggagacg accttccatg
      601 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
      661 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
      721 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
      781 ggcaaggtgt gggtcgcgga cg
//
