LOCUS Exported 5696 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pXP122 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5696) AUTHORS Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, Hatfield GW, Da Silva NA, Sandmeyer S TITLE A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. JOURNAL Yeast. 2010 Sep 10. ():. PUBMED 20936606 REFERENCE 2 (bases 1 to 5696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5696 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 364..386 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 378..395 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(440..473) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(1915..1948) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(2478..2494) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2478..2494) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2491..2513) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2502..2518 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2526..2556) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2571..2592 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2709..2726) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2880..3468) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2960..2979) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3639..4499) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4262..4281 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" terminator 4524..4771 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind 4737..4755 /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind 5669..5687 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 421 actctagagg atccccggga taacttcgta taatgtatgc tatacgaagt tatggatttt 481 cttaacttct tcggcgacag catcaccgac ttcggtggta ctgttggaac cacctaaatc 541 accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg ccttaccttc 601 ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag tggcgatagg 661 gttgacctta ttctttggca aatctggagc agaaccgtgg catggttcgt acaaaccaaa 721 tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc 781 tgggataacg gaggcttcat cggagatgat atcaccaaac atgttgctgg tgattataat 841 accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa tcaattgatg 901 ttgaaccttc aatgtaggaa attcgttctt gatggtttcc tccacagttt ttctccataa 961 tcttgaagag gccaaaacat tagctttatc caaggaccaa ataggcaatg gtggctcatg 1021 ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa cggtgtattg 1081 ttcactatcc caagcgacac catcaccatc atcttccttt ctcttaccaa agtaaatacc 1141 tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg gcttgattgg 1201 agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg cgtacaattg 1261 aagttcttta cggattttta gtaaaccttg ttcaggtcta acactacctg taccccattt 1321 aggaccaccc acagcaccta acaaaacggc atcagccttc ttggaggctt ccagcgcctc 1381 atctggaagt gggacacctg tagcttcgat agcagcacca ccaattaaat gattttcgaa 1441 atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa tggcttcggc 1501 tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag gggcagacat 1561 tagaatggta tatccttgaa atatatatat atattgctga aatgtaaaag gtaagaaaag 1621 ttagaaagta agacgattgc taaccaccta ttggaaaaac aataggtcct taaataatat 1681 tgtcaacttc aagtattgtg atgcaagcat ttagtcatga acgcttctct attctatatg 1741 aaaagccggt tccggcgctc tcacctttcc tttttctccc aatttttcag ttgaaaaagg 1801 tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc cagtcatcga atttgattct 1861 gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa aaaaataatg gttaataact 1921 tcgtataatg tatgctatac gaagttatcc cgggtaccga gctcgaattc gtaacttaca 1981 cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt 2041 ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga 2101 agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata 2161 tttattagac aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg 2221 taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat 2281 taatacctga gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga 2341 gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct 2401 atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg 2461 atgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 2521 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 2581 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 2641 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 2701 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 2761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 2821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 2881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 2941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 3001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 3061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 3121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 3181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 3241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 3301 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 3361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 3421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 3481 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 3541 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 3601 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 3661 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 3721 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 3781 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 3841 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 3901 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 3961 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 4021 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 4081 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 4141 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 4201 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 4261 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 4321 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 4381 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 4441 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 4501 ctcttccttt ttcaatattg gccgcaaatt aaagccttcg agcgtcccaa aaccttctca 4561 agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa 4621 atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc 4681 tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt 4741 gaatgtaagc gtgacataac taattacatg actcgagtgc gcagatatca ctagtatatt 4801 tgttgtaaaa agtagataat tacttccttg atgatctgta aaaaagagaa aaagaaagca 4861 tctaagaact tgaaaaacta cgaattagaa aagaccaaat atgtatttct tgcattgacc 4921 aatttatgca agtttatata tatgtaaatg taagtttcac gaggttctac taaactaaac 4981 cacccccttg gttagaagaa aagagtgtgt gagaacaggc tgttgttgtc acacgattcg 5041 gacaattctg tttgaaagag agagagtaac agtacgatcg aacgaacttt gctctggaga 5101 tcacagtggg catcatagca tgtggtacta aaccctttcc cgccattcca gaaccttcga 5161 ttgcttgtta caaaacctgt gagccgtcgc taggaccttg ttgtgtgacg aaattggaag 5221 ctgcaatcaa taggaagaca ggaagtcgag cgtgtctggg ttttttcagt tttgttcttt 5281 ttgcaaacaa atcacgagcg acggtaattt ctttctcgat aagaggccac gtgctttatg 5341 agggtaacat caattcaaga aggagggaaa cacttccttt ttctggccct gataatagta 5401 tgagggtgaa gccaaaataa aggattcgcg cccaaatcgg catctttaaa tgcaggtatg 5461 cgatagttcc tcactctttc cttactcacg agtaattctt gcaaatgcct aatattattg 5521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 5581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 5641 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc //