LOCUS Exported 5479 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Luciferase reporter. ACCESSION . VERSION . KEYWORDS pSGE_662_S2-1-Tor_dCP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5479) AUTHORS Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A TITLE Transcriptional regulators form diverse groups with context-dependent regulatory functions. JOURNAL Nature. 2015 Nov 9. doi: 10.1038/nature15545. PUBMED 26550828 REFERENCE 2 (bases 1 to 5479) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5479 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 39..160 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(50..69) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 104..123 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(408..425) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(579..1167) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(659..678) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1338..2198) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1961..1980 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2199..2303) /gene="bla" /label=AmpR promoter rep_origin 2330..2785 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2417..2436) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2627..2648 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 2916..2964 /note="synthetic polyadenylation signal" misc_feature 2978..3069 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 3018..3037 /label=RVprimer3 /note="pGL3 vector, forward primer" protein_bind 3083..3107 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3604..3628) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 3819..3828 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3825..5477 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(3891..3909) /label=LucNrev /note="Firefly luciferase, reverse primer" ORIGIN 1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg 61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag 301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 361 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 2221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2761 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa 2821 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt 2881 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac 2941 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca 3001 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa 3061 catttctcta tcgataggta ccacaagttt gtacaaaaaa gcaggctccg aattcgccct 3121 ttgcacaatt tctgtgaagt gacagccact gagaaatagt gtcaagtagc tctcgtccgc 3181 taatatcaat ttagacgata tacatacggc cagatctggc acgcagtctg cactcggagg 3241 acagtactcc ggcgggatgg atttggattg tggggcggaa tctctaagtg cgataagatg 3301 cggagaatta cttccgcgac ttggctccgt tttgttaaat atactatgtg tgtatgtgcg 3361 tagtacagcg acttgtacgg agtacataaa catatgcata tctgcggttt agttgtcgac 3421 attatctttt gttcgtcgtt tgaaaaatgt gtgtagttgt tggggtaact gcgcacaatt 3481 gccgggctac agggtgcttt gagtctttgt ttttggattt cgttttcgat gtttagctac 3541 gaaagccaag cgttgtgcta ttttcgaatt ttgtatttta taacccgggc aagggcgaat 3601 tcgacccagc tttcttgtac aaagtggtag atctgcgatc gagctcgccc ggggatcgag 3661 cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg aatgttcgag 3721 ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact ttgaatcaca 3781 agacgcatac caaacggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa 3841 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac 3901 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac 3961 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag 4021 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc 4081 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga 4141 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg 4201 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca 4261 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca 4321 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct 4381 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta 4441 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta 4501 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg 4561 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt 4621 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc 4681 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt 4741 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag 4801 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta 4861 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg 4921 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc 4981 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg 5041 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact 5101 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag 5161 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg 5221 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg 5281 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag 5341 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc 5401 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg 5461 gaaagatcgc cgtgtaatt //