LOCUS Exported 5510 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Luciferase reporter. ACCESSION . VERSION . KEYWORDS pSGE_648_S2-1-Trl_dCP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5510) AUTHORS Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A TITLE Transcriptional regulators form diverse groups with context-dependent regulatory functions. JOURNAL Nature. 2015 Nov 9. doi: 10.1038/nature15545. PUBMED 26550828 REFERENCE 2 (bases 1 to 5510) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5510 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 39..160 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(50..69) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 104..123 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(408..425) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(579..1167) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(659..678) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1338..2198) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1961..1980 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2199..2303) /gene="bla" /label=AmpR promoter rep_origin 2330..2785 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2417..2436) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2627..2648 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 2916..2964 /note="synthetic polyadenylation signal" misc_feature 2978..3069 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 3018..3037 /label=RVprimer3 /note="pGL3 vector, forward primer" protein_bind 3083..3107 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3635..3659) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 3850..3859 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3856..5508 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(3922..3940) /label=LucNrev /note="Firefly luciferase, reverse primer" ORIGIN 1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg 61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag 301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 361 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 2221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2761 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa 2821 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt 2881 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac 2941 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca 3001 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa 3061 catttctcta tcgataggta ccacaagttt gtacaaaaaa gcaggctccg aattcgccct 3121 tcacaatttc tgtgaagtga cagccctcgg aggacagtac tccggtgtca agctcggagg 3181 acagtactcc gcgctaatat caatttagac gatatacata cggccagatc tggcacgcag 3241 tctgcattcc caatgatgcg ggatggattt ggattgtggg gcggaactcg gaggacagta 3301 ctccgaagtg cgataagatg ctcggaggac agtactccga ttacttccgc gacttggctc 3361 cgttttgtta aatatactat gtgtgtatgt gcgtagtaca gcgacttgta cggagtacat 3421 aaacatatgc atatctgcgg tttagttgtc gacattatct tttgttcgtc gtttgaaaaa 3481 tgtgtgtagt tgttggggta actgcgcaca attgccgggc tacagggtgc tttgagtctt 3541 tgtttttgga tttcgttttc gatgtttagc tacgaaagcc aagcgttgtg ctattttcga 3601 attttgtatt ttataacccg gaagggcgaa ttcgacccag ctttcttgta caaagtggta 3661 gatctgcgat cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc 3721 tgagagcatc agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa 3781 tatcctttga ataagccaac tttgaatcac aagacgcata ccaaacggca ttccggtact 3841 gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat 3901 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg 3961 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag 4021 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat 4081 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg 4141 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc 4201 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa 4261 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa 4321 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt 4381 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc 4441 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc 4501 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact 4561 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat 4621 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg 4681 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc 4741 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt 4801 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt 4861 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg 4921 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat 4981 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct 5041 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat 5101 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt 5161 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa 5221 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat 5281 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa 5341 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga 5401 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc 5461 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt //