LOCUS Exported 6101 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Luciferase reporter. ACCESSION . VERSION . KEYWORDS pSGE_539_2xUAS_dCP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6101) AUTHORS Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A TITLE Transcriptional regulators form diverse groups with context-dependent regulatory functions. JOURNAL Nature. 2015 Nov 9. doi: 10.1038/nature15545. PUBMED 26550828 REFERENCE 2 (bases 1 to 6101) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6101 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(19..879) /codon_start=1 /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" polyA_signal 984..1032 /note="synthetic polyadenylation signal" misc_feature 1046..1137 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 1086..1105 /label=RVprimer3 /note="pGL3 vector, forward primer" protein_bind 1184..1208 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(1273..1297) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 1494..1503 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1500..3152 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(1566..1584) /label=LucNrev /note="Firefly luciferase, reverse primer" polyA_signal 3193..3314 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3204..3223) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3258..3277 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 3508..3865 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3531..3551) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3716..3851 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3778..3797 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3896..4933 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK EVGRV" RBS 4912..4920 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" polyA_signal 4957..5005 /note="synthetic polyadenylation signal" primer_bind complement(5161..5178) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5332..5920) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5412..5431) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gctaaaccac tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg 61 cctatttcgt tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga 121 gggcttacca tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc 181 cgatttgtca gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac 241 tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc 301 agtgagtagt ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc 361 gttcggtatg gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc 421 catattatga agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt 481 ggccgcggtg ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc 541 atccgtaaga tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg 601 tatacggcga ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag 661 cagtactttg aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat 721 cttgccgcta ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc 781 atcttttact ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa 841 gaagggaatg agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta 901 ttgaagcatt tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt 961 acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg 1021 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa 1081 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctggc 1141 ctaactggcc ggtacctgag ctcgctagcc tcgaggatat caaacaagtt tgtacaaaaa 1201 agcaggcttc cggagtactg tcctccgagc ggaagcttgc atgcctgcag gtcggagtac 1261 tgtcctccga ggacccagct ttcttgtaca aagtggtata tcaagatctg cgatcgagct 1321 cgcccgggga tcgagcgcag cggtataaaa gggcgcgggg tggctgagag catcagttgt 1381 gaatgaatgt tcgagccgag cagacgtgcc gctgccttcg ttaatatcct ttgaataagc 1441 caactttgaa tcacaagacg cataccaaac ggcattccgg tactgttggt aaagccacca 1501 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa 1561 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg 1621 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg 1681 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat 1741 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg 1801 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt 1861 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa 1921 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat 1981 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt 2041 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat 2101 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc 2161 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg 2221 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc 2281 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca 2341 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga 2401 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta 2461 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg 2521 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg 2581 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa 2641 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt 2701 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg 2761 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc 2821 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac 2881 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc 2941 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt 3001 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg 3061 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa 3121 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc 3181 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 3241 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 3301 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg 3361 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc 3421 cgtttgcgta ttgggcgctc ttccgctgat ctgcgcagca ccatggcctg aaataacctc 3481 tgaaagagga acttggttag ctaccttctg aggcggaaag aaccagctgt ggaatgtgtg 3541 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 3601 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 3661 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 3721 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 3781 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 3841 ttttggaggc ctaggctttt gcaaaaagct cgattcttct gacactagcg ccaccatgaa 3901 gaagcccgaa ctcaccgcta ccagcgttga aaaatttctc atcgagaagt tcgacagtgt 3961 gagcgacctg atgcagttgt cggagggcga agagagccga gccttcagct tcgatgtcgg 4021 cggacgcggc tatgtactgc gggtgaatag ctgcgctgat ggcttctaca aagaccgcta 4081 cgtgtaccgc cacttcgcca gcgctgcact acccatcccc gaagtgttgg acatcggcga 4141 gttcagcgag agcctgacat actgcatcag tagacgcgcc caaggcgtta ctctccaaga 4201 cctccccgaa acagagctgc ctgctgtgtt acagcctgtc gccgaagcta tggatgctat 4261 tgccgccgcc gacctcagtc aaaccagcgg cttcggccca ttcgggcccc aaggcatcgg 4321 ccagtacaca acctggcggg atttcatttg cgccattgct gatccccatg tctaccactg 4381 gcagaccgtg atggacgaca ccgtgtccgc cagcgtagct caagccctgg acgaactgat 4441 gctgtgggcc gaagactgtc ccgaggtgcg ccacctcgtc catgccgact tcggcagcaa 4501 caacgtcctg accgacaacg gccgcatcac cgccgtaatc gactggtccg aagctatgtt 4561 cggggacagt cagtacgagg tggccaacat cttcttctgg cggccctggc tggcttgcat 4621 ggagcagcag actcgctact tcgagcgccg gcatcccgag ctggccggca gccctcgtct 4681 gcgagcctac atgctgcgca tcggcctgga tcagctctac cagagcctcg tggacggcaa 4741 cttcgacgat gctgcctggg ctcaaggccg ctgcgatgcc atcgtccgca gcggggccgg 4801 caccgtcggt cgcacacaaa tcgctcgccg gagcgcagcc gtatggaccg acggctgcgt 4861 cgaggtgctg gccgacagcg gcaaccgccg gcccagtaca cgaccgcgcg ctaaggaggt 4921 aggtcgagtt taaactctag aaccggtcat ggccgcaata aaatatcttt attttcatta 4981 catctgtgtg ttggtttttt gtgtgttcga actagatgct gtcgaccgat gcccttgaga 5041 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 5101 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc 5161 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5221 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5281 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5341 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5401 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5461 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5521 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5581 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 5641 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 5701 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 5761 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 5821 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 5881 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5941 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6001 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6061 aagtatatat gagtaaactt ggtctgacag cggccgcaaa t //