LOCUS Exported 5484 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Luciferase reporter. ACCESSION . VERSION . KEYWORDS pSGE_722_S2-1_CGCG-to-HEY SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5484) AUTHORS Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A TITLE Transcriptional regulators form diverse groups with context-dependent regulatory functions. JOURNAL Nature. 2015 Nov 9. doi: 10.1038/nature15545. PUBMED 26550828 REFERENCE 2 (bases 1 to 5484) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5484 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 39..160 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(50..69) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 104..123 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(408..425) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(579..1167) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(659..678) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1338..2198) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1961..1980 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2199..2303) /gene="bla" /label=AmpR promoter rep_origin 2330..2785 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2417..2436) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2627..2648 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 2916..2964 /note="synthetic polyadenylation signal" misc_feature 2978..3069 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 3018..3037 /label=RVprimer3 /note="pGL3 vector, forward primer" protein_bind 3083..3107 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3609..3633) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 3824..3833 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3830..5482 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(3896..3914) /label=LucNrev /note="Firefly luciferase, reverse primer" ORIGIN 1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg 61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag 301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 361 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 1741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 1801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 1861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 1921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 1981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 2221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt 2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 2761 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa 2821 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt 2881 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac 2941 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca 3001 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa 3061 catttctcta tcgataggta ccacaagttt gtacaaaaaa gcaggctccg aattcgccct 3121 ttgcacaatt tctgtgaagt gacagccact gagaaatagt gtcaagtagc tctcgtccgc 3181 taatatcaat ttagacgata tacatacggc cagatctggc acgcagtctg cattcccaat 3241 gatgcgggat ggatttggat tgtggggcgg aatctctaag tgcgataaga tgcggagaat 3301 taggggcacg tgtcggctgt ggctccgttt tgttaaatat actatgtgtg tatgtgcgta 3361 gtacagcgac ttgtacggag tacataaaca tatgcatatc tgcggtttag ttgtcgacat 3421 tatcttttgt tcgtcgtttg aaaaatgtgt gtagttgttg gggtaggggc acgtgtcggc 3481 tgattgccgg gctacagggt gctttgagtc tttgtttttg gatttcgttt tcgatgttta 3541 gctacgaaag ccaagcgttg tgctattttc gaattttgta ttttataacc cgggcaaggg 3601 cgaattcgac ccagctttct tgtacaaagt ggtagatctg cgatcgagct cgcccgggga 3661 tcgagcgcag cggtataaaa gggcgcgggg tggctgagag catcagttgt gaatgaatgt 3721 tcgagccgag cagacgtgcc gctgccttcg ttaatatcct ttgaataagc caactttgaa 3781 tcacaagacg cataccaaac ggcattccgg tactgttggt aaagccacca tggaagacgc 3841 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga 3901 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga 3961 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc 4021 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa 4081 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc 4141 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac 4201 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc 4261 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat 4321 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga 4381 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct 4441 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga 4501 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca 4561 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt 4621 aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag 4681 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata 4741 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg 4801 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga 4861 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa 4921 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt 4981 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa 5041 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc 5101 ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta 5161 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat 5221 cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt 5281 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag 5341 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa 5401 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa 5461 gggcggaaag atcgccgtgt aatt //