LOCUS Exported 7209 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBF3038 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7209) AUTHORS Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, Hatfield GW, Da Silva NA, Sandmeyer S TITLE A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. JOURNAL Yeast. 2010 Sep 10. ():. PUBMED 20936606 REFERENCE 2 (bases 1 to 7209) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7209 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 2..443 /gene="S. cerevisiae GAL1" /label=GAL1 promoter /note="inducible promoter, regulated by Gal4" protein_bind 2..119 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" primer_bind 414..437 /label=GAL1 /note="S. cerevisiae GAL1 promoter, forward primer" terminator 1627..1874 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(1643..1661) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind complement(1944..1961) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2115..2703) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2195..2214) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2874..3734) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" CDS complement(3752..4846) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" rep_origin 5788..6668 /label=2u ori /note="yeast 2u plasmid origin of replication" rep_origin complement(6737..7192) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 7086..7105 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt 61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga 121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac 181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga 241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat 301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc 361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac 421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gccatggaaa 481 aaaatgtcta atttgttgac tgttcatcaa aatttgccag ctttgccagt tgatgcaacg 541 tctgatgagg taagaaagaa tttgatggat atgtttagag atagacaagc attctctgaa 601 catacttgga aaatgttgtt gtctgtttgt agatcttggg ctgcttggtg taaattaaat 661 aatagaaaat ggtttccagc tgaaccagaa gatgttagag attatttgtt gtatttgcaa 721 gctagaggtt tggctgttaa aactatacaa caacatttgg gtcaattaaa tatgttgcat 781 aggagatctg gtttgcctag accatctgat tctaatgctg tatctttggt tatgagaagg 841 attagaaaag aaaatgttga tgcaggtgaa agagctaaac aagcattggc gtttgaaaga 901 actgattttg atcaagttag atctttgatg gaaaattctg atagatgtca agatataaga 961 aacttggctt tcttgggaat tgcttataat acattgttga gaattgctga aattgctaga 1021 attagagtta aagacatttc tagaactgat ggtggtagaa tgttgattca tattggtaga 1081 actaaaacat tggtttcaac tgctggcgtt gagaaagcat tgtctttggg tgttactaaa 1141 ttggttgaaa gatggatttc tgtatctggt gtggctgatg acccaaataa ttatttattt 1201 tgtagagtta gaaagaatgg tgttgcggct ccatctgcta catctcagct ctctacccgt 1261 gccttggaag gaatttttga agcaactcat agattgattt atggtgctaa agatgattct 1321 ggtcaaagat atttagcatg gtctggtcat tctgctagag ttggtgccgc tagagatatg 1381 gctagagctg gggtatctat tcctgaaatt atgcaagctg gtggttggac taatgttaac 1441 atcgtcatga actatattag aaatttggat tctgaaactg gtgcaatggt tagactcttg 1501 gaagatggtg atcccgggaa gcttggtacc gagctcggat ccactagtaa cggccgccag 1561 tgtgctggaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg 1621 gccgcatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc 1681 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag 1741 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac 1801 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga 1861 aggctttaat ttgcggccct gcattaatga atcggccaac gcgcggggag aggcggtttg 1921 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 1981 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 2041 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagc ccaggaaccg taaaaaggcc 2101 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 2161 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 2281 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 2341 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 2401 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 2461 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 2521 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 2581 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 2641 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 2701 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 2761 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 2821 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 2881 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 2941 tgactccccg tcgtgtagat aactacgata cgggagcgct taccatctgg ccccagtgct 3001 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 3061 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat tcagtctatt 3121 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 3181 ggcattgcta caggcatcgt ggtgtcactc tcgtcgtttg gtatggcttc attcagctcc 3241 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 3301 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 3361 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 3421 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 3481 ccggcgtcaa tacgggataa tagtgtatca catagcagaa ctttaaaagt gctcatcatt 3541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 3601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 3661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 3721 tgttgaatac tcatactctt cctttttcaa tttaagcaag gattttctta acttcttcgg 3781 cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac 3841 ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca 3901 atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg accttattct 3961 ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt 4021 ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg 4081 cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg 4141 ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg 4201 taggaaattc gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca 4261 aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga 4321 aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag 4381 cgacaccatc accatcatct tcctttctct taccaaagta aatacctccc actaattctc 4441 tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa 4501 gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga 4561 tttttagtaa accttgttca ggtctaacac tacctgtacc ccatttagga ccacccacag 4621 cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggga 4681 cacctgtagc ttcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat 4741 tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac 4801 caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc 4861 cttgaaatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac 4921 gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag 4981 tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc 5041 ggcctctcac ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgtcaggc 5101 gacctctgaa attaacaaaa aatttccagt catcgaattt gattctgtgc gatagcgccc 5161 ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggtta tcgatgataa gctgtcaaag 5221 atgagaatta attccacgga ctatagacta tactagatac tccgtctact gtacgataca 5281 cttccgctca ggtccttgtc ctttaacgag gccttaccac tcttttgtta ctctattgat 5341 ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt aaaactagct 5401 agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc atcattatta 5461 tccgatgtga cgctgcagct tctcaatgat attcgaatac gctttgagga gatacagcct 5521 aatatccgac aaactgtttt acagatttac gatcgtactt gttacccatc attgaatttt 5581 gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa 5641 tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt 5701 gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat 5761 cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa 5821 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa 5881 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa 5941 aacaaaaatg caacgcgacg agagcgctaa tttttcaaac aaagaatctg agctgcattt 6001 ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt 6061 ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta 6121 ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg 6181 tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct 6241 gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata 6301 aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag 6361 tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc 6421 tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga 6481 atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg 6541 tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg 6601 gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta 6661 ttcgcaatgg gaagctccac cccggttgat aatcagaaaa gccccaaaaa caggaagatt 6721 gtataagcaa atatttaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt 6781 tgttaaatca gctcattttt taacgaatag cccgaaatcg gcaaaatccc ttataaatca 6841 aaagaataga ccgagatagg gttgagtgtt gttccagttt ccaacaagag tccactatta 6901 aagaacgtgg actccaacgt caaagggcga aaaagggtct atcagggcga tggcccacta 6961 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc agtaaatcgg 7021 aagggtaaac ggatgccccc atttagagct tgacggggaa agccggcgaa cgtggcgaga 7081 aaggaaggga agaaagcgaa aggagcgggg gctagggcgg tgggaagtgt aggggtcacg 7141 ctgggcgtaa ccaccacacc cgccgcgctt aatggggcgc tacagggcgc gtggggatga 7201 tccactagt //