LOCUS Exported 7099 bp ds-DNA circular SYN 16-JUN-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA3-Egr1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7099) AUTHORS Yu J, de Belle I, Liang H, Adamson ED TITLE Coactivating factors p300 and CBP are transcriptionally crossregulated by Egr1 in prostate cells, leading to divergent responses. JOURNAL Mol Cell. 2004 Jul 2. 15(1):83-94. PUBMED 15225550 REFERENCE 2 (bases 1 to 7099) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 16, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7099 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 959..982 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" promoter complement(2652..2670) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2653..2670) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2690..2707) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2696..2920 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2966..3394 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3053..3072) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3263..3284 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3403..3423) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3408..3737 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3588..3723 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3650..3669 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3804..4598 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3858..3877) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4468..4487 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4772..4893 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4809..4828) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4863..4882 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4942..4958) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4942..4958) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4955..4977) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4966..4982 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4990..5020) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5035..5056 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5173..5190) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5344..5932) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5424..5443) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6103..6963) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6726..6745 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6964..7068) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcttatgga 961 ctacaaggac gacgatgaca aagtcaagct tgcagcggcc aaggccgaga tgcaattgat 1021 gtctccgctg cagatctctg acccgttcgg ctcctttcct cactcaccca ccatggacaa 1081 ctaccccaaa ctggaggaga tgatgctgct gagcaacggg gctccccagt tcctcggtgc 1141 tgccggaacc ccagagggca gcggcggtaa tagcagcagc agcaccagca gcgggggcgg 1201 tggtgggggc ggcagcaaca gcggcagcag cgccttcaat cctcaagggg agccgagcga 1261 acaaccctat gagcacctga ccacagagtc cttttctgac atcgctctga ataatgagaa 1321 ggcgatggtg gagacgagtt atcccagcca aacgactcgg ttgcctccca tcacctatac 1381 tggccgcttc tccctggagc ccgcacccaa cagtggcaac actttgtggc ctgaacccct 1441 tttcagccta gtcagtggcc tcgtgagcat gaccaatcct ccgacctctt catcctcggc 1501 gccttctcca gctgcttcat cgtcttcctc tgcctcccag agcccgcccc tgagctgtgc 1561 cgtgccgtcc aacgacagca gtcccatcta ctcggctgcg cccacctttc ctactcccaa 1621 cactgacatt tttcctgagc cccaaagcca ggcctttcct ggctcggcag gcacagcctt 1681 gcagtacccg cctcctgcct accctgccac caaaggtggt ttccaggttc ccatgatccc 1741 tgactatctg tttccacaac aacagggaga cctgagcctg ggcaccccag accagaagcc 1801 cttccagggt ctggagaacc gtacccagca gccttcgctc actccactat ccactattaa 1861 agccttcgcc actcagtcgg gctcccagga cttaaaggct cttaatacca cctaccaatc 1921 ccagctcatc aaacccagcc gcatgcgcaa gtaccccaac cggcccagca agacaccccc 1981 ccatgaacgc ccatatgctt gccctgtcga gtcctgcgat cgccgctttt ctcgctcgga 2041 tgagcttacc cgccatatcc gcatccacac aggccagaag cccttccagt gtcgaatctg 2101 catgcgtaac ttcagtcgta gtgaccacct taccacccac atccgcaccc acacaggcga 2161 gaagcctttt gcctgtgaca tttgtgggag gaagtttgcc aggagtgatg aacgcaagag 2221 gcataccaaa atccatttaa gacagaagga caagaaagca gacaaaagtg tggtggcctc 2281 cccggctgcc tcttcactct cttcttaccc atccccagtg gctacctcct acccatcccc 2341 tgccaccacc tcattcccat cccctgtgcc cacttcctac tcctctcctg gctcctccac 2401 ctacccatct cctgcgcaca gtggcttccc gtcgccgtca gtggccacca cctttgcctc 2461 cgttccacct gctttcccca cccaggtcag cagcttcccg tctgcgggcg tcagcagctc 2521 cttcagcacc tcaactggtc tttcagacat gacagcgacc ttttctccca ggacaattga 2581 aattttgctg agtcgagaat tcccatccat cacactggcg gccgctcgag catgcatcta 2641 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg 2701 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 2761 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 2821 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 2881 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 2941 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 3001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 3061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 3121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 3181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 3241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 3301 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 3361 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 3421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3481 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3541 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 3601 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3661 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3721 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 3781 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3841 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3901 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3961 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 4021 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 4081 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 4141 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 4201 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 4261 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 4321 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 4381 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 4441 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 4501 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 4561 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4621 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4681 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4741 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 4801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 4861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 4921 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4981 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 5041 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 5101 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 5161 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 5221 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5281 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5341 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5401 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5461 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5521 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 5581 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5641 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 5701 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5761 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 5821 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 5941 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6001 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6061 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6241 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 6421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 6481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 6541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 6601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 6661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 6721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 6781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 6841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 6901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 6961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7081 aaaagtgcca cctgacgtc //