LOCUS Exported 5556 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.. ACCESSION . VERSION . KEYWORDS POM43-mCitrine SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5556) AUTHORS Higuchi-Sanabria R, Garcia EJ, Tomoiaga D, Munteanu EL, Feinstein P, Pon LA TITLE Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae. JOURNAL PLoS One. 2016 Jan 4;11(1):e0146120. doi: 10.1371/journal.pone.0146120. eCollection 2016. PUBMED 26727004 REFERENCE 2 (bases 1 to 5556) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5556 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(53..86) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(2375..2408) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(3193..3212) /label=T7 /note="T7 promoter, forward primer" promoter complement(3194..3212) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3299..3316) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3470..4058) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3550..3569) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4229..5089) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4852..4871 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5090..5194) /gene="bla" /label=AmpR promoter primer_bind 5262..5280 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(5318..5340) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 5440..5459 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 5540..2 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 5540..1 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acaaccctta atataacttc 61 gtataatgta tgctatacga agttattagg tctagagatc cgcaggctaa ccggaacctg 121 tattatttag tttatgctac gttaaataaa gacctttcgt tcacataact gaatgtgtaa 181 tggccttgag atttcaagca taccaagttg gtggagacgg ggtcgttaca aaagactctt 241 taagccaaga tttccttgac agccttggcg atagcatcgc caacctcagt ggtagagtta 301 gaaccaccaa ggtcaccggt tctgacacct gcatccaaga catttctaac agcttcttca 361 agagccctac cttcttcaac caaatccaag gataacttca acatcatagc tgcagataag 421 atggtagcaa ttgggttaac cttgtttgct ggtaaatctg gggcagaacc atgacatggt 481 tcgtacaaac cgaatgcctt gttagtgtca ggtagggaag ctagagatgc agaaggtaat 541 aaacccaaag aacctggaat aacagaggct tcatcggaga taatatcacc aaacatgttg 601 ttggtaataa caacaccgtt tagcttagtt ggtgatttaa ccaaaatcat agcagcagag 661 tcgatcaatt ggtgctgaac agttaattgt gggaactcag tcttgatggt ttcttcaaca 721 gtctttctcc acaatctgga agaggcaagc acgttagcct tgtcaagaga ccagattggt 781 aatggtgggt tttgttgcaa tgccaagaaa gcagccattc ttgtaattct ttgaacttca 841 ggaacactgt atttctcaga gtcccaagca actccgtcac cttcatcttc ttttctttca 901 ccaaagtaga taccaccaac caattctcta acgacgacga aatcggtacc ctttgcatat 961 tcaggcttca aaggagaaag atctagtaaa gaatcagaag caaagttaca tggtctcaag 1021 ttggcgtata gacccaattc ctttctgatc ttcaatagac cttgttctgg tctaactgcg 1081 cccgtacccc attttggacc accaacagca cctagtaaga cagcatcggc tttcttagag 1141 gcttctagag cttcatctgg taaaggagtg ccagtggcat cgatggcagc acccccgatc 1201 aagtgatgtt ggaaattgaa cttaatttct ggacggactt cagcaatggc attcaagacc 1261 ttaatagctt cgtcagtaac ttctttaccg acgtgatcac ccggtaggac aacgatattc 1321 ttagacatgg tataatctgt gtagtgtggg atacttttta cttcttcaaa taggtatcaa 1381 caataaaaat aaatcgaagc aaatgtagga atgcgttaaa gcagatgtac ttacttagag 1441 tacatagata tatttatata attcaatata taaaagtata tgaagcatct gatgttgaac 1501 ctgtcatgac tctaaactgg aatgggtagt tatgcttctg aaggttttcc gttagaaaat 1561 gagtgcaggg atcaggcccg gaaccggttt tagcctgaaa ggaaaagaaa aaaatgcggt 1621 gattactagc acgtgactgc gctgaattgg aatccaccac aagtacagcg aatggcaaga 1681 gggaagggga atatattgtg cctggcggta gtctgctata tcaaatgtta aacataacta 1741 aatgcagtag tgaatagagt aatgtatttc tacatatgtg ggtagggcca atgggagccc 1801 gatgtctatt gtacagatat tttcctttta tagttaatca attcaacttg gaaatcattg 1861 agtcgatatc tacttggata actggagccg tggcagcgtt tgaaatttga cgagtcttct 1921 tgttttgttc gcgcattttc agtttattca aacgggagat ggcaccgaat ccactgactt 1981 ctctgttcaa tttgaatggt aataggaaga aagtacattt cgagaaattt tcgcccaacg 2041 tcgaaatctc atggatgata tcctcgatac aaatgacacc ttcatcacct aatttctctt 2101 caacgatatt gttgtcattc aagattatct cttttggttc tttatcttct ggcctttgcc 2161 acattattct agatctcttt tggattaaag aacggataga tgcaagtgaa ggttgtccga 2221 taacaatata tggtgataca agtttcaaca atgggtacac gtccttggta agtttgatga 2281 aaacaccagt gttcaactca acaagtctca aaagagataa cactttgtaa gcctttgctg 2341 ggatcttcac agcgagctct cgagaaccct taatataact tcgtataatg tatgctatac 2401 gaagttatta ggtgatatca tgcgtacgct gcaggtcgac ggatcctcta aaggtgaaga 2461 attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta atggtcacaa 2521 attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga ccttaaaatt 2581 tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta ctttaggtta 2641 tggtttgatg tgttttgcta gatacccaga tcatatgaaa caacatgact ttttcaagtc 2701 tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg acggtaacta 2761 caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa tcgaattaaa 2821 aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat acaactataa 2881 ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa 2941 aattagacac aacattgaag atggttctgt tcaattagct gaccattatc aacaaaatac 3001 tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcct atcaatctag 3061 attatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat ttgttactgc 3121 tgctggtatt acccatggta ttgatgaatt gtacaaaact agtggcctat gcggccgcgg 3181 atctgccggt ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt 3241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 3301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3661 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3901 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 4561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 4621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 4681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 4741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 4801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 4861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 4921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 4981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 5101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 5161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5221 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 5281 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 5341 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 5401 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 5461 tgagagtgca ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct 5521 tatgtatcat acacatacga tttaggtgac actata //