LOCUS       Exported                 820 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Provides the CDS of the Yellow fluorescente protein as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    pYFP (GB0053)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(128..149)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(389..408)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             548..799
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     736..757
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa ttcactagtg
       61 attgggcgat gagtggtctc gaatggtgag caagggtgaa gagctgttca ccggggtggt
      121 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      181 gggcgagggc gacgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      241 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc
      301 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      361 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      421 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      481 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      541 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      601 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      661 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
      721 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      781 catggacgag ctctacaagt cagtcatgag ctgagcttca
//
