LOCUS Exported 3790 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pYFP (GB0053) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3790) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3790 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(24..275) /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VC155 /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" primer_bind complement(66..87) /label=EGFP-C /note="EGFP, forward primer" primer_bind 415..434 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 674..695 /label=EGFP-N /note="EGFP, reverse primer" promoter complement(840..858) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(841..858) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(876..892) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(876..892) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(889..911) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 900..916 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(924..954) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 969..990 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1107..1124) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1278..1866) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1358..1377) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2037..2897) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2660..2679 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2898..3002) /gene="bla" /label=AmpR promoter rep_origin complement(3080..3535) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3217..3238) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3429..3448 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3661..3683 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3675..3692 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3676..3692 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3699..3718 /label=T7 /note="T7 promoter, forward primer" promoter 3699..3717 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3748..3757 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3752..3761 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgaagctc agctcatgac tgacttgtag agctcgtcca tgccgagagt gatcccggcg 61 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg 121 gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg 181 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc 241 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg 301 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc 361 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg 421 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg 481 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca cttcaggccg 541 tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg 601 aacttcaggg tcagcttgcc gtaggtggcg tcgccctcgc cctcgccgga cacgctgaac 661 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc 721 tcttcaccct tgctcaccat tcgagaccac tcatcgccca atcactagtg aattcgcggc 781 cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt 841 ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 901 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 961 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 1021 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 1081 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 1141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 1201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 1261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 1321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 1381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 1441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 1501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 1561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 1621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 1681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 1741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 1801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 1861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 1921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 1981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 2041 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 2101 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 2161 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag 2221 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 2281 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 2341 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 2401 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 2461 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 2521 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 2581 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 2641 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 2701 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 2761 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 2821 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 2881 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 2941 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 3001 cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag 3061 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 3121 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 3181 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 3241 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgctggccca 3301 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 3361 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 3421 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 3481 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt 3541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 3601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 3661 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg 3721 aattgggccc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattgggc 3781 gatgagtggt //