LOCUS Exported 6655 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pmCherry-CIBN-CreC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6655) AUTHORS Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL TITLE Rapid blue-light-mediated induction of protein interactions in living cells. JOURNAL Nat Methods. 2010 Oct 31. ():. PUBMED 21037589 REFERENCE 2 (bases 1 to 6655) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6655 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 607..616 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 613..1320 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(758..776) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1026..1045 /label=mCherry-F /note="mCherry, forward primer" primer_bind complement(1922..1938) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1923..1939) /label=pBluescriptKS /note="For pBluescript vector" CDS 1950..2459 /codon_start=1 /product="N-terminal portion of the CIB1 transcription factor from Arabidopsis thaliana" /label=CIBN /note="binds to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)" /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE LEKTDYI" polyA_signal 3358..3479 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3395..3414) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3449..3468 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3486..3941) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3623..3644) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3835..3854 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3968..4072 /gene="bla" /label=AmpR promoter promoter 4074..4431 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4097..4117) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4282..4417 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4344..4363 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4466..5260 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4520..4539) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5130..5149 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5448..5467) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5492..5539 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5868..6456 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6357..6376 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tgtggcggct agtactccgg tattgcggta cccttgtacg cctgttttat actcccttcc 1381 cgtaacttag acgcacaaaa ccaagttcaa tagaaggggg tacaaaccag taccaccacg 1441 aacaagcact tctgtttccc cggtgatgtc gtatagactg cttgcgtggt tgaaagcgac 1501 ggatccgtta tccgcttatg tacttcgaga agcccagtac cacctcggaa tcttcgatgc 1561 gttgcgctca gcactcaacc ccagagtgta gcttaggctg atgagtctgg acatccctca 1621 ccggtgacgg tggtccaggc tgcgttggcg gcctacctat ggctaacgcc atgggacgct 1681 agttgtgaac aaggtgtgaa gagcctattg agctacntaa gaatcctccg gcccctgaat 1741 gcggctaatc ccaacctcgg agcaggtggt cacaaaccag tgattggcct gtcgtaacgc 1801 gcaagtccgt ggcggaaccg actactttgg gtgtccgtgt ttccttttat tttattgtgg 1861 ctgcttatgg tgacaatcac agattgttat cataaagcga attggatagg atcaagctta 1921 tcgataccgt cgacctcgag ctcgccacca tgaatggagc tataggaggt gaccttttgc 1981 tcaattttcc tgacatgtcg gtcctagagc gccaaagggc tcacctcaag tacctcaatc 2041 ccacctttga ttctcctctc gccggcttct ttgccgattc ttcaatgatt accggcggcg 2101 agatggacag ctatctttcg actgccggtt tgaatcttcc gatgatgtac ggtgagacga 2161 cggtggaagg tgattcaaga ctctcaattt cgccggaaac gacgcttggg actggaaatt 2221 tcaagaaacg gaagtttgat acagagacta aggattgtaa tgagaagaag aagaagatga 2281 cgatgaacag agatgaccta gtagaagaag gagaagaaga gaagtcgaaa ataacagagc 2341 aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca caaagccaag aaagaagaga 2401 acaatttctc taatgattca tctaaagtga cgaaggaatt ggagaaaacg gattatattc 2461 atggtggcgg tggctctgga ggtggtgggt ccggaggagg cggccgccga ccaagtgaca 2521 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac 2581 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg 2641 aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca 2701 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg 2761 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag 2821 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg 2881 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc 2941 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc 3001 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca 3061 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca 3121 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata 3181 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagcccggg atccaccgga 3241 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 3301 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 3361 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3421 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 3481 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 3541 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 3601 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3661 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3721 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3781 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3841 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3901 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 3961 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 4021 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 4081 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4141 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4201 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4261 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4321 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 4381 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 4441 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 4501 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 4561 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 4621 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 4681 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 4741 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 4801 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 4861 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 4921 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 4981 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 5041 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 5101 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 5161 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 5221 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 5281 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 5341 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 5401 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 5461 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 5521 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 5581 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 5641 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 5701 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 5761 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 5821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 5881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 5941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 6001 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 6061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 6121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 6181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 6241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 6301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 6361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 6421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 6481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 6541 tctgtggata accgtattac cgccatgcat tagcaccgcc tacatacctc gctctgctaa 6601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttgga //