LOCUS Exported 3184 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pUPD_Mini35s(-60,UTR) (GB0050) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3184) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3184) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3184 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 13..59 /label=minimal CaMV 35S promoter /note="minimal 35S promoter from cauliflower mosaic virus" promoter complement(234..252) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(235..252) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(270..286) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(270..286) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(283..305) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 294..310 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(318..348) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 363..384 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(501..518) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(672..1260) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(752..771) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1431..2291) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2054..2073 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2292..2396) /gene="bla" /label=AmpR promoter rep_origin complement(2474..2929) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2611..2632) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2823..2842 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3055..3077 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3069..3086 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3070..3086 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3093..3112 /label=T7 /note="T7 promoter, forward primer" promoter 3093..3111 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3142..3151 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3146..3155 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgtcccct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac 61 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac 121 tattctagtc gaaatgcgag accactcatc gcccatcact agtgaattcg cggccgcctg 181 caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag 241 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 301 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 361 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 481 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 541 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 601 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 661 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 841 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 1021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 1081 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 1141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 1201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 1261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 1321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 1381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 1441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 1501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 1561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 1621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 1681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 1741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 1801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 1861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 1921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 1981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 2041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 2101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 2161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 2221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 2281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 2341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 2401 tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa 2461 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat 2941 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 3001 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 3061 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg 3121 gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt gggcgatgag 3181 tggt //