LOCUS       Exported               16840 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCG31
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16840)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 16840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(9956..9972)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10026..10054)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10802..10822
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             10908..10928
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10941..10985
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    10998..11022
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11404..11428)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(14649..14667)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14688..14704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14688..14704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14701..14723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14712..14728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14736..14766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(14919..14936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15090..15678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(15849..16709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16472..16491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16710..16814)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt
      721 tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc
      781 aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct
      841 ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt
      901 cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga aatccttata
      961 tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca
     1021 aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga
     1081 aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca
     1141 gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt
     1201 gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg
     1261 cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat
     1321 atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa
     1381 accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt
     1441 caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca
     1501 gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag ataaaatcct
     1561 aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct
     1621 ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca
     1681 caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc
     1741 atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt
     1801 ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc
     1861 tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg agcaagagca
     1921 cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta
     1981 ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc
     2041 ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt
     2101 gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta
     2161 attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa
     2221 aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc
     2281 ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat
     2341 tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca
     2401 attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat
     2461 tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca
     2521 aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg
     2581 tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat
     2641 ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa tttttatttt
     2701 aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta
     2761 tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa
     2821 tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat
     2881 atgaaggcag agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag
     2941 gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc
     3001 ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt
     3061 tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta
     3121 atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa
     3181 cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta
     3241 gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt
     3301 ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg
     3361 ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc
     3421 aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa
     3481 cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt
     3541 gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc
     3601 aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa
     3661 tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc
     3721 ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc
     3781 cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa
     3841 aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa
     3901 aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat
     3961 aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc
     4021 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     4081 acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta
     4141 ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg
     4201 ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga
     4261 cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca
     4321 ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt
     4381 aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg
     4441 aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg
     4501 attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc
     4561 acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc
     4621 gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt
     4681 caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt
     4741 caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga
     4801 cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac
     4861 attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa
     4921 gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat
     4981 tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa
     5041 aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata
     5101 gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg
     5161 cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg
     5221 tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg
     5281 cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa
     5341 atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa
     5401 aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa
     5461 atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga
     5521 tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa
     5581 atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa
     5641 acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag
     5701 ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat
     5761 ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca
     5821 ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt
     5881 tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt
     5941 tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca
     6001 tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag
     6061 gagaagcctc cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac
     6121 catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa
     6181 agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa aattttccga
     6241 catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt
     6301 gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt
     6361 cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg
     6421 gccgcgcgga agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa
     6481 taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa
     6541 attttgaaaa acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt
     6601 ttttccgtcg ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg
     6661 aaaattcgag ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt
     6721 tctcgttaaa tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc
     6781 gcaatggatg atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc
     6841 aatttttcgt gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc
     6901 acattttggt agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact
     6961 actaaaagat gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca
     7021 cggaaaattg agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt
     7081 aggcggtttt gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt
     7141 atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat
     7201 tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt
     7261 aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa
     7321 aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat
     7381 ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc
     7441 aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt
     7501 tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat
     7561 gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca
     7621 gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga ttctcggaaa
     7681 aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt tttcgtatnt
     7741 tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn aatttaattg
     7801 aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc ggttaataac
     7861 gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg acgtttttaa
     7921 atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat gacaaaggaa
     7981 taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac gtggattttt
     8041 ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag gattattgta
     8101 gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat tctcagaatt
     8161 ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca aaaacaattt
     8221 ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct aattatattt
     8281 gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc attaattaat
     8341 tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt ttatcgggaa
     8401 atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat tagtatttct
     8461 cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt cttgtatatt
     8521 tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa taatgaaaat
     8581 taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt tcaattggcg
     8641 gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa aatcatataa
     8701 catctagaca atttttttga atttttttat catgatattc ggtcattgtg tccccatagg
     8761 catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg tcaaaagaca
     8821 tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt ttggaggtga
     8881 aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag aaaatcgaca
     8941 tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact ggaaaatctg
     9001 ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat ttgttcgaaa
     9061 tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt ttttgcttgg
     9121 gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct tcagcgtacg
     9181 gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga aaatatcgga
     9241 aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga gaaaaatgct
     9301 tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa atctgtgagt
     9361 atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac caaattcgga
     9421 ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac gtttcttttc
     9481 gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat tatataaaaa
     9541 tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca attaaatata
     9601 attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta aataatcagt
     9661 gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct gtgagtcccg
     9721 caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggcggca
     9781 aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt ctttttgcag
     9841 tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagatggct caaacaaagc
     9901 cgattgccga gcaaatggga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
     9961 ccgtcgacct cgagggggcg gtaccggtag aaaaaatgag taaaggagaa gaacttttca
    10021 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
    10081 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
    10141 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
    10201 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
    10261 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
    10321 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
    10381 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
    10441 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
    10501 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
    10561 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
    10621 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
    10681 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
    10741 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
    10801 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
    10861 aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
    10921 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
    10981 acagcggagg tggaggtaca agtttgtaca aaaaagcagg cttaatggac ttgcccgatc
    11041 cctatttacc cggagcaata agcttgttcg agcagcttga caaaaaatta ttagttgttc
    11101 tccgcgacgg acggaaattg attggcttct tgagatcgat agatcaattt gccaatctta
    11161 tattggaaga tgtcgtagaa cgcacatttg ttgagaaata cttctgtgaa acgggtcaag
    11221 gattcatgct tattcgcgga gagaatgttg agcttgctgg agaaatcgat gataccattg
    11281 agactggact tacgcaggtg tctcccgaag aatttcgtcg gctggaggat gagtatattg
    11341 ccaaaaatcc gccaaagttc ctaaaacgac aagcagagaa aacagaagaa cacgatgcat
    11401 gaaacccagc tttcttgtac aaagtggttg atctagtgga tcctaatttt gccgtatttt
    11461 ccatattttg ttttgtatat ttatccactc accccctctc tttgtcctgt gaatgaactt
    11521 gtgccaaaaa agccaaaaat aatttacttt ttttaaagac atattaaaac ttgaaaactt
    11581 ggaacaatac ggtgcgaaag cgaaacagag acagaaaaat gaacaaagag atccgtgaaa
    11641 agtgaacgat gatttaggct tcttgttggg cggcgtggat gagagtgatg ttgtctcctt
    11701 tgagaagaat tctgccgatc ttgttgcgtc ccttcgtctt catgttcacc tcctcggctt
    11761 cgtcgaacac gacattcatg aactcgtcga atccgatgat gtagccctcg agacggtggg
    11821 tgacatcctc gtacaaccag atttggaccc gagtgcggtt ttggagatat ctgaaatttg
    11881 ggaaattgtt ttctactgat tagtgaaaaa atattacctg aagatgaggt tcacaggctg
    11941 aaccatcact ttgttgagct ttctcgtcga catttttgcg ggatgatctg taaaaatcaa
    12001 tatatttttt gaaagacaca aatttttaag ataaatatca agaaaactcg attttagcag
    12061 ctatttaagc tcaaaaacta caatttatgg ctttaaaaca taatttttca cattttacat
    12121 aaatttcaac gaaaaaaaat gccaaaaaac ttcaaatttg agcagaaatc ggctattttc
    12181 tcgacaaaaa taatttcgaa aattcgttca aaaaagatga gtacgcaagc accgtgacgc
    12241 acagttgcgg actttgtaaa ccgtacccct gcataaaaag gcggaatttt gaatttaata
    12301 aaatgcagtt tagaacaaag aaaagcacgg agagaattgt gataactaat tatttgtatt
    12361 taattttaaa tactacataa aactgaagat tggaacgagt taaagctggt atttgcggtt
    12421 tatagcccca aaagttaagg cttgttaggc aattatcgat aaaaacaacg agaaattcgt
    12481 gaaatcggca aaaatttata gaaaacgttt gaattaaatt aaatttacgc ttttgccgcc
    12541 aacacctatc gagaccctag ttataggggc ggagcgtcat gggagcgcgc ttgcaactcg
    12601 atttcaaaaa gtttcgctgt ttgtacaaca ttctcagttg ttcttaatca gttaaataag
    12661 tttcaaaatt ttnncaaaat cataaaacac agttaatgct aaattaaagt ctaaaaanat
    12721 caggtgataa ggggtttgga cgaccaaaaa gcgaccttcg ctcgaaaatt gataaactaa
    12781 ctacactttt tccggaaaaa tgatggaaaa atcctgattt tcttcgttga atcaattttt
    12841 gtttcatttt taacactttc acatgannaa tatttcagtt aaaatgctca agctaattgg
    12901 gtttcttata attttgggtc tcgcaacggc cgacaagtgc tcagattgtt tggctcaggg
    12961 gaaacagtga gttggaagct tacaattctg agaattcgta ttgcgcagct tatttgacgc
    13021 gcaaaatatg aaatatcacg tagcgaaaac tacagtaatt ctttaaataa gactactgta
    13081 gtgcctatgt cgatttactg cctcaacttt cttaatcaag ctcgtaaatc gacaccagct
    13141 ctacagtagt catagttttc gctacgagat attttgcgcg tcaaatatgg tgtgcaatac
    13201 gcattttcag aattttatgt tctcgtaata tacctttttt cagctggtgt gttactgtaa
    13261 atgcatgtgg atttacgcca tgtgtcacta caattacagt gagttttttt gaagaaaaaa
    13321 aaattaccca tttgatcgcc aattccattt ttcaaaaatc aataaaaaaa aaaaatttcg
    13381 gcgatcagaa acgttacaga aagccctaaa ctgcccggaa ctaccagata aagcccacgc
    13441 ttacgatgat tcatttatga ggaataaggt attgatcaca cattctgctg cctacaatat
    13501 ggacccacaa aagtgtttta cgtaggttca ccttctagat aaaccttaaa aatcgatact
    13561 ttcagtagta aaatgccttc gatgaaagtt tccaaaacag ttaatgtcaa ttgtgataga
    13621 tatgggccaa gtgagttgtt ttattagccc acggatttct ggcttccctc ataaattgaa
    13681 aaggaagaga ttgccgaact agaccatttt ggctcggcgc gttgcgtgtc acgtcgcggc
    13741 tcgattgtaa aactaaatgt attggtccgt gtggagtaca cgactttccc tttccggcag
    13801 gcgattgtca atggagcgcg aaaaattcaa tgaggaaggc cagaaccccg tgtctgtagt
    13861 tttctcaaaa agtggcaaaa actcaataat tgccaatttt tgactgccaa taaaaatttc
    13921 actaaattta ttaaatttat tttaagtttt attgaaattt ttttaaaatt tccccactgg
    13981 cgctaaaaaa tccaattttc agaagcaatg tgcttcagct tcacggccat agatactgtc
    14041 caaaaagtga ttgcaatgac gtttagaggg acagaaggag cagctcaatt gtctgaagaa
    14101 attttggatt tcttccagta agttcgggaa atggcaattt aaagtttaat tggatttttg
    14161 tattttttct tctcacgact acagttttgc acatttttat gttataaaaa accaaaaaaa
    14221 aatttttttg ccttttttct cagcttacca caaaacaatt tcgaccaaaa ataaattgaa
    14281 aaaattaaaa aattttttgc acggttttcg gactcaaaaa aagctgaatg ttcctggaac
    14341 tatttgtaaa attcaaaatt caaaattttc acgaattctc taaaaggttc cagcaaattc
    14401 cacaactttc ttgaattttt caaaaaagtt cttgagcatt caacaattat ttttggaatt
    14461 ttccaaaggt tacaggacgt tccaattttt caaaaaaatt tctcgcaaaa aatgttttct
    14521 cagaaaattt gaatttccct ccacataaaa gattgttctc aaaaaatttg caaacacaca
    14581 ttttcagcgg aaaaaaggcg ttcttcgact ctggaagtgt attcgaatat ggtacccagc
    14641 ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc atagctgttt
    14701 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg aagcataaag
    14761 tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg
    14821 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    14881 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    14941 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    15001 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    15061 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tcggcccccc tgacgagcat
    15121 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    15181 gcgttccccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    15241 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
    15301 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    15361 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    15421 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    15481 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    15541 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    15601 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    15661 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    15721 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    15781 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    15841 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    15901 tcatccatag ttgcctgact gcccgtcgtg tagataacta cgatacggga gggcttacca
    15961 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    16021 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    16081 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    16141 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    16201 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtga
    16261 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    16321 ttatcactca tgcttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    16381 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    16441 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    16501 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    16561 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    16621 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    16681 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    16741 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    16801 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
