LOCUS Exported 3332 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the 4xLexA Operator as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pLexAOp (GB0118) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3332) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3332) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3332 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(382..400) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(383..400) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(418..434) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(418..434) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(431..453) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 442..458 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(466..496) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 511..532 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(649..666) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(820..1408) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(900..919) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1579..2439) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2202..2221 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2440..2544) /gene="bla" /label=AmpR promoter rep_origin complement(2622..3077) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2759..2780) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2971..2990 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3203..3225 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3217..3234 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3218..3234 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3241..3260 /label=T7 /note="T7 promoter, forward primer" promoter 3241..3259 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3290..3299 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3294..3303 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgggagag cttgggctgc aggtcgaggc taaaaaacta atcgcattat catcccctcg 61 acgtactgta catataacca ctggttttat atacagcagt actgtacata taaccactgg 121 ttttatatac agcagtcgac gtactgtaca tataaccact ggttttatat acagcagtac 181 tgtacatata accactggtt ttatatacag cagtcgaggt aagattagat atggatatgt 241 atatggatat gtatatggtg gtaatgccat gtaatatgct ccccgagacc actcatcgcc 301 caatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg 361 ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc 421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 1021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 1081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 1141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 1201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 1261 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 1321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 1381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 1441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 1501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 1561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 1621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 1681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 1741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 1801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 1861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 1921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 1981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 2041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 2101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 2161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 2221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 2281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 2341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 2401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 2461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 2521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg 2581 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat 2641 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 2701 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 2761 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 2821 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 2881 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2941 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 3001 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 3061 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg 3121 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 3181 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg 3241 taatacgact cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc 3301 ggccgcggga attcgattgg gcgatgagtg gt //