LOCUS Exported 3328 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pGal4BD::BDR (GB0734) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3328) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3328) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3328 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(378..396) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(379..396) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(414..430) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(414..430) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(427..449) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 438..454 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(462..492) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 507..528 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(645..662) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(816..1404) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(896..915) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1575..2435) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGPRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2198..2217 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2436..2540) /gene="bla" /label=AmpR promoter rep_origin complement(2618..3073) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2755..2776) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2967..2986 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3199..3221 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3213..3230 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3214..3230 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3237..3256 /label=T7 /note="T7 promoter, forward primer" promoter 3237..3255 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3286..3295 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3290..3299 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgaatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc 61 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc 121 gctactctcc caaaaccaaa agatctccgc tgactagggc acatctgaca gaagtggaat 181 caaggctaga aagactggaa cagctatttc tactgatttt tcctaggggt aattctaaga 241 ctcttagact ttttggtgtt aatatggaat gttgagcttc gagaccactc atcgcccaat 301 cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg 361 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag 421 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 481 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 541 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 601 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 1021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 1081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 1141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 1201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 1261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 1321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 1381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 1441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1501 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1561 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 1621 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 1681 ttaccatctg gccccagtgc tgcaatgata ccgcgaggcc cacgctcacc ggctccagat 1741 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 1801 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 1861 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 1921 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 1981 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 2041 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 2101 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 2161 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 2221 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 2281 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 2341 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 2401 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 2461 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 2521 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat 2581 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg 2641 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 2701 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 2761 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 2821 tatcagggcg ctggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 2881 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 2941 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 3001 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 3061 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 3121 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 3181 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat 3241 acgactcact atagggcgaa ttgggcccga cgtcgcatgc tcccggccgc catggcggcc 3301 gcgggaattc gattgggcga tgagtggt //