LOCUS Exported 884 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance. ACCESSION . VERSION . KEYWORDS pCfB2336 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 884) AUTHORS Stovicek V, Borja GM, Forster J, Borodina I TITLE EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains. JOURNAL J Ind Microbiol Biotechnol. 2015 Sep 16. PUBMED 26376869 REFERENCE 2 (bases 1 to 884) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..884 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 76..265 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" protein_bind complement(313..346) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 401..744 /label=TEF promoter /note="Ashbya gossypii TEF promoter" ORIGIN 1 aatcataaga aattcgctta tttagaagtg tcaacaacgt atctaccaac ggaatgcgtg 61 cgatcgcgtg cattcatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta 121 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat 181 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct 241 tgagaaggtt ttgggacgct cgaagatcgc gtcagctgaa gcttcgtacg ctgcaggtcg 301 acaaccctta atataacttc gtataatgta tgctatacga agttattagg tctagagatc 361 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac 421 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat 481 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc 541 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac 601 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt 661 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca 721 catcacatcc gaacataaac aaccatgggt aggagggctt ttgtagaaag aaatacgaac 781 gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc ccttacctga agagtcgaat 841 tttattgatg aacttataac ttccaagcat gcaaaccaaa aggg //