LOCUS       Exported               12693 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    pCXLE-hSK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12693)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 Apr 3. ():.
  PUBMED    21460823
REFERENCE   2  (bases 1 to 12693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12693
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2718..2783
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    4271..4859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4324..4344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4742..4753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4949..4968)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5014..5069
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5068..5087)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5430..5446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5430..5446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5443..5465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5454..5470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5478..5508)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5523..5544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(5633..5666)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             6127..8052
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    complement(8220..8253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      8440..10229
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(10771..10788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10942..11530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11022..11041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11701..12561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12324..12343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12562..12666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
     1741 ccatgtacaa catgatggag acggagctga agccgccggg cccgcagcaa acttcggggg
     1801 gcggcggcgg caactccacc gcggcggcgg ccggcggcaa ccagaaaaac agcccggacc
     1861 gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg cgggcagcgg cgcaagatgg
     1921 cccaggagaa ccccaagatg cacaactcgg agatcagcaa gcgcctgggc gccgagtgga
     1981 aacttttgtc ggagacggag aagcggccgt tcatcgacga ggctaagcgg ctgcgagcgc
     2041 tgcacatgaa ggagcacccg gattataaat accggccccg gcggaaaacc aagacgctca
     2101 tgaagaagga taagtacacg ctgcccggcg ggctgctggc ccccggcggc aatagcatgg
     2161 cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt gaaccagcgc atggacagtt
     2221 acgcgcacat gaacggctgg agcaacggca gctacagcat gatgcaggac cagctgggct
     2281 acccgcagca cccgggcctc aatgcgcacg gcgcagcgca gatgcagccc atgcaccgct
     2341 acgacgtgag cgccctgcag tacaactcca tgaccagctc gcagacctac atgaacggct
     2401 cgcccaccta cagcatgtcc tactcgcagc agggcacccc tggcatggct cttggctcca
     2461 tgggttcggt ggtcaagtcc gaggccagct ccagcccccc tgtggttacc tcttcctccc
     2521 actccagggc gccctgccag gccggggacc tccgggacat gatcagcatg tatctccccg
     2581 gcgccgaggt gccggaaccc gccgccccca gcagacttca catgtcccag cactaccaga
     2641 gcggcccggt gcccggcacg gccattaacg gcacactgcc cctctcacac atgggggatc
     2701 tcaaaattgt cgctcctgtc aaacaaactc ttaactttga tttactcaaa ctggctgggg
     2761 atgtagaaag caatccaggt ccactcatga acaattcgcc cttcaccatg gctgtcagtg
     2821 acgcgctgct cccatctttc tccacgttcg cgtctggccc ggcgggaagg gagaagacac
     2881 tgcgtcaagc aggtgccccg aataaccgct ggcgggagga gctctcccac atgaagcgac
     2941 ttcccccagt gcttcccggc cgcccctatg acctggcggc ggcgaccgtg gccacagacc
     3001 tggagagcgg cggagccggt gcggcttgcg gcggtagcaa cctggcgccc ctacctcgga
     3061 gagagaccga ggagttcaac gatctcctgg acctggactt tattctctcc aattcgctga
     3121 cccatcctcc ggagtcagtg gccgccaccg tgtcctcgtc agcgtcagcc tcctcttcgt
     3181 cgtcgccgtc gagcagcggc cctgccagcg cgccctccac ctgcagcttc acctatccga
     3241 tccgggccgg gaacgacccg ggcgtggcgc cgggcggcac gggcggaggc ctcctctatg
     3301 gcagggagtc cgctccccct ccgacggctc ccttcaacct ggcggacatc aacgacgtga
     3361 gcccctcggg cggcttcgtg gccgagctcc tgcggccaga attggacccg gtgtacattc
     3421 cgccgcagca gccgcagccg ccaggtggcg ggctgatggg caagttcgtg ctgaaggcgt
     3481 cgctgagcgc ccctggcagc gagtacggca gcccgtcggt catcagcgtc agcaaaggca
     3541 gccctgacgg cagccacccg gtggtggtgg cgccctacaa cggcgggccg ccgcgcacgt
     3601 gccccaagat caagcaggag gcggtctctt cgtgcaccca cttgggcgct ggaccccctc
     3661 tcagcaatgg ccaccggccg gctgcacacg acttccccct ggggcggcag ctccccagca
     3721 ggactacccc gaccctgggt cttgaggaag tgctgagcag cagggactgt caccctgccc
     3781 tgccgcttcc tcccggcttc catccccacc cggggcccaa ttacccatcc ttcctgcccg
     3841 atcagatgca gccgcaagtc ccgccgctcc attaccaaga gctcatgcca cccggttcct
     3901 gcatgccaga ggagcccaag ccaaagaggg gaagacgatc gtggccccgg aaaaggaccg
     3961 ccacccacac ttgtgattac gcgggctgcg gcaaaaccta cacaaagagt tcccatctca
     4021 aggcacacct gcgaacccac acaggtgaga aaccttacca ctgtgactgg gacggctgtg
     4081 gatggaaatt cgcccgctca gatgaactga ccaggcacta ccgtaaacac acggggcacc
     4141 gcccgttcca gtgccaaaaa tgcgaccgag cattttccag gtcggaccac ctcgccttac
     4201 acatgaagag acatttttaa aagggcgaat tgcctgcagg aattcaagct tcggggacta
     4261 gtcatatgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4321 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4381 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4441 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4501 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4561 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4621 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4681 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4741 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4801 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     4861 tcggtaaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     4921 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     4981 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     5041 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     5101 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     5161 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     5221 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     5281 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     5341 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     5401 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5521 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5581 aacctgtcgt gccagcggat ctcaattccg atcatattca ataaccctta atataacttc
     5641 gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct
     5701 taggatcaat tctcatgttt gacagcttat catcgataag ctgatcctca caggccgcac
     5761 ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg
     5821 aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag gaatttgagg
     5881 cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt tccgggctgc
     5941 gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca gacctggatc
     6001 tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg ggcaggagtc
     6061 tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc tcttttagtg
     6121 tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg agagaaggga
     6181 gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg gggtgataac
     6241 catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg agccccgggc
     6301 ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa acgtccaagt
     6361 tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc gggaggggca
     6421 ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
     6481 ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg
     6541 gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga
     6601 gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
     6661 ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
     6721 ggaggagggg caggagcagg aggggcagga ggggcaggag gggcaggagc aggaggggca
     6781 ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga
     6841 ggggcaggag caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
     6901 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
     6961 ggagcaggag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     7021 ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg aggggcagga
     7081 gcaggagggg caggagcagg aggtggaggc cggggtcgag gaggcagtgg aggccggggt
     7141 cgaggaggta gtggaggccg gggtcgagga ggtagtggag gccgccgggg tagaggacgt
     7201 gaaagagcca gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg acgtggagaa
     7261 aagaggccca ggagtcccag tagtcagtca tcatcatccg ggtctccacc gcgcaggccc
     7321 cctccaggta gaaggccatt tttccaccct gtaggggaag ccgattattt tgaataccac
     7381 caagaaggtg gcccagatgg tgagcctgac gtgcccccgg gagcgataga gcagggcccc
     7441 gcagatgacc caggagaagg cccaagcact ggaccccggg gtcagggtga tggaggcagg
     7501 cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag gaggttccaa cccgaaattt
     7561 gagaacattg cagaaggttt aagagctctc ctggctagga gtcacgtaga aaggactacc
     7621 gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag gtagtaagac ctccctttac
     7681 aacctaaggc gaggaactgc ccttgctatt ccacaatgtc gtcttacacc attgagtcgt
     7741 ctcccctttg gaatggcccc tggacccggc ccacaacctg gcccgctaag ggagtccatt
     7801 gtctgttatt tcatggtctt tttacaaact catatatttg ctgaggtttt gaaggatgcg
     7861 attaaggacc ttgttatgac aaagcccgct cctacctgca atatcagggt gactgtgtgc
     7921 agctttgacg atggagtaga tttgcctccc tggtttccac ctatggtgga aggggctgcc
     7981 gcggagggtg atgacggaga tgacggagat gaaggaggtg atggagatga gggtgaggaa
     8041 gggcaggagt gatgtaactt gttaggagac gccctcaatc gtattaaaag ccgtgtattc
     8101 ccccgcacta aagaataaat ccccagtaga catcatgcgt gctgttggtg tatttctggc
     8161 catctgtctt gtcaccattt tcgtcctccc aacatggggc aattgccgga acccttaata
     8221 taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgaagagg ttcactagcg
     8281 gatctcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca acaccttctc
     8341 gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga cggctttagc
     8401 ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga caagcagcga
     8461 aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc cactctacta
     8521 ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc ggatacagat
     8581 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
     8641 taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
     8701 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
     8761 taggatagca tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg
     8821 gtagtatatg ctacccagat ataaattagg atagcatata ctaccctaat ctctattagg
     8881 atagcatatg ctacccggat acagattagg atagcatata ctacccagat atagattagg
     8941 atagcatatg ctacccagat atagattagg atagcctatg ctacccagat ataaattagg
     9001 atagcatata ctacccagat atagattagg atagcatatg ctacccagat atagattagg
     9061 atagcctatg ctacccagat atagattagg atagcatatg ctatccagat atttgggtag
     9121 tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt gaatatgagg
     9181 accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc
     9241 agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc ccggggtgcc
     9301 attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta caatcagcca
     9361 agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg
     9421 tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt atgggcccca
     9481 ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg agtccgctgc
     9541 tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat ggttcacctg
     9601 tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca ctaggattat
     9661 gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg gcttgtcccc
     9721 accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca ctagtattta
     9781 ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac cactgaaccc
     9841 cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc acctcacata
     9901 caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag aatgaagaag
     9961 caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac tgcccttgtg
    10021 actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc
    10081 tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta attcgatagc
    10141 atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga tagtatatac
    10201 tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt ataaacacta
    10261 ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct ctgattgacg
    10321 ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc ccagcattgg
    10381 tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag tccacagact
    10441 gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca tccatttata
    10501 aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt gtcacatgtg
    10561 gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg cactgccccg
    10621 cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg gcatcgatcc
    10681 tctagagtcg acgctagcgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
    10741 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    10801 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    10861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11341 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    11401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    11641 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    11761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    11821 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    11881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12001 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    12361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    12421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    12481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    12541 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    12601 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12661 tccgcgcaca tttccccgaa aagtgccacc tgg
//
