LOCUS Exported 756 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven. ACCESSION . VERSION . KEYWORDS pL-CRISPR.EFS.GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 756) AUTHORS Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL TITLE Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. JOURNAL Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. PUBMED 24952903 REFERENCE 2 (bases 1 to 756) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..756 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 125..144 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 221..296 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 344..461 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 530..741 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" ORIGIN 1 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa 61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa 121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat 181 cttgtggaaa ggacgaaaca ccggagacgt gaccgtctct gttttagagc tagaaatagc 241 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt 301 ttgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg 361 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 421 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 481 gcagagatcc actttggcgc cggctcgagt ggctccggtg cccgtcagtg ggcagagcgc 541 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag 601 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 661 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 721 gggtttgccg ccagaacaca ggtgtcgtga cgcggg //