LOCUS       Exported                5071 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Transcriptional unit for Renilla Protein plant expression driven by 
            the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pEGB 35S:Renilla:Tnos (GB0109)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1033..1968
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     terminator      1995..2247
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2282..2300)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2488..2512
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2603..3191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2683..2702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3362..4177)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4085..4104
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4169..4188
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4468..4903
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5036..5058
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatgacttc gaaagtttat gatccagaac aaaggaaacg gatgataact
     1081 ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt tattaattat
     1141 tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct
     1201 tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca
     1261 gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat
     1321 cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt
     1381 gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca tcaagataag
     1441 atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg
     1501 cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg
     1561 gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca
     1621 gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca
     1681 ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt tgtacaaatt
     1741 gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa
     1801 tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact
     1861 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
     1921 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatg agcttggaat
     1981 ggatcttcga tcccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2041 ttgccggtct tgcgacgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2101 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2161 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2221 gcdcggtgtc atctatgtta ctagatcggg aattgccaag ctaattcttg aagacgaaag
     2281 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2341 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2401 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatgggac
     2461 cgactcgcgc tgtcatgaga cgaattctga caggatatat tggcgggtaa actaagtcgc
     2521 tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2761 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3001 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3061 gcgctctgct gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac
     3121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgtgtaacat tggtctagtg
     3361 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     3421 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     3481 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     3541 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     3601 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     3661 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     3721 gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     3781 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     3841 attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat
     3901 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3961 ttagtctgac catctcatct gtaacaacat tggcaacgct acctttgcca tgtttcagaa
     4021 acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga
     4081 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     4141 gccttgagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     4201 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     4261 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     4321 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa
     4381 tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc
     4441 tggatgatgg ggcgattcag gcgatcccca tccaacagcc cgccgtcgag cgggcttttt
     4501 tatccccgga agcctgtgga tagagggtag ttatccacgt gaaaccgcta atgccccgca
     4561 aagccttgat tcacggggct ttccggcccg ctccaaaaac tatccacgtg aaatcgctaa
     4621 tcagggtacg tgaaatcgct aatcggagta cgtgaaatcg ctaataaggt cacgtgaaat
     4681 cgctaatcaa aaaggcacgt gagaacgcta atagcccttt cagatcaaca gcttgcaaac
     4741 acccctcgct ccggcaagta gttacagcaa gtagtatgtt caattagctt ttcaattatg
     4801 aatatatata tcaattattg gtcgcccttg gcttgtggac aatgcgctac gcgcaccggc
     4861 tccgcccgtg gacaaccgca agcggttgcc caccgtcgag cgccagcgcc tttgcccaca
     4921 acccggcggc cggccgcaac agatcgtttt ataaattttt ttttttgaaa aagaaaaagc
     4981 ccgaaaggcg gcaacctctc gggcttctgg atttccgatc cccggaatta gatcttggca
     5041 ggatatattg tggtgtaacg aattcgtctc a
//
