LOCUS       Exported                5782 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Transcriptional unit for Firefly Luciferase Protein plant expression
            driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pEGB 35S:Luciferase:Tnos (GB0110)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1033..2685
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1099..1117)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2712..2964
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2999..3017)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3205..3229
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3320..3908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3400..3419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4079..4894)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4802..4821
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4886..4905
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5185..5620
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5747..5769
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
     1081 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     1141 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
     1201 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     1261 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     1321 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     1381 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
     1441 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
     1501 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     1561 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
     1621 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
     1681 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     1741 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     1801 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
     1861 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
     1921 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
     1981 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
     2041 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     2101 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     2161 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
     2221 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     2281 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
     2341 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
     2401 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
     2461 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     2521 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     2581 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     2641 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaatgagc ttggaatgga
     2701 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     2761 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     2821 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     2881 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcd
     2941 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc
     3001 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3061 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     3181 ctcgcgctgt catgagacga attctgacag gatatattgg cgggtaaact aagtcgctgt
     3241 atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3721 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3781 ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa
     3841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4021 attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg tctagtgatt
     4081 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     4141 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     4201 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     4261 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     4321 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     4381 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     4441 cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     4501 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     4561 cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac catgcatcat
     4621 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     4681 gtctgaccat ctcatctgta acaacattgg caacgctacc tttgccatgt ttcagaaaca
     4741 actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat tgcccgacat
     4801 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     4861 ttgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     4921 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     4981 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     5041 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     5101 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
     5161 atgatggggc gattcaggcg atccccatcc aacagcccgc cgtcgagcgg gcttttttat
     5221 ccccggaagc ctgtggatag agggtagtta tccacgtgaa accgctaatg ccccgcaaag
     5281 ccttgattca cggggctttc cggcccgctc caaaaactat ccacgtgaaa tcgctaatca
     5341 gggtacgtga aatcgctaat cggagtacgt gaaatcgcta ataaggtcac gtgaaatcgc
     5401 taatcaaaaa ggcacgtgag aacgctaata gccctttcag atcaacagct tgcaaacacc
     5461 cctcgctccg gcaagtagtt acagcaagta gtatgttcaa ttagcttttc aattatgaat
     5521 atatatatca attattggtc gcccttggct tgtggacaat gcgctacgcg caccggctcc
     5581 gcccgtggac aaccgcaagc ggttgcccac cgtcgagcgc ctttgcccac aacccggcgg
     5641 ccggccgcaa cagatcgttt tataaatttt tttttttgaa aaagaaaaag cccgaaaggc
     5701 ggcaacctct cgggcttctg gatttccgat ccccggaatt agatcttggc aggatatatt
     5761 gtggtgtaac gaattcgtct ca
//
