LOCUS       Exported                4812 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Transcriptional unit for DsRed plant expression driven by the 35S 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pEGB 35S:DsRed:Tnos (GB0361)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1034..1711
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1214..1234)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1626..1649
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      1735..1987
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2022..2040)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2224..2248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2339..2927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2419..2438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3098..3913)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3821..3840
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3905..3924
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4204..4639
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    4772..4794
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt
     1081 gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1141 cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt
     1201 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
     1261 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1321 gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg
     1381 ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat
     1441 gcagaagaag acgatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     1501 gctgaagggc gagacacaca aggccctgaa gctgaaggac ggcggccact acctggtgga
     1561 gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga
     1621 cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg
     1681 caccgagggc cgccaccacc tgttcctgta ggcttggaat ggatcttcga tcccgatcgt
     1741 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgacgatt
     1801 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     1861 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     1921 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcdcggtgtc atctatgtta
     1981 ctagatcggg aattgccaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt
     2041 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2101 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2161 catgagacaa taaccctgat aaatgcttca ataatgggac cgactcgcgc ttgagacgaa
     2221 gcttgacagg atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatctca
     2281 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2341 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2401 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2461 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2521 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2581 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2641 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2701 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2761 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2821 tcggaagaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2881 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2941 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3001 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3061 caatctaaag tatatatgtg taacattggt ctagtgatta gaaaaactca tcgagcatca
     3121 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     3181 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     3241 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     3301 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     3361 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     3421 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc
     3481 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     3541 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     3601 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     3661 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     3721 caacattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     3781 catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     3841 catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt
     3901 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     3961 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     4021 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     4081 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
     4141 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga
     4201 tccccatcca acagcccgcc gtcgagcggg cttttttatc cccggaagcc tgtggataga
     4261 gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc cttgattcac ggggctttcc
     4321 ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag ggtacgtgaa atcgctaatc
     4381 ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct aatcaaaaag gcacgtgaga
     4441 acgctaatag ccctttcaga tcaacagctt gcaaacaccc ctcgctccgg caagtagtta
     4501 cagcaagtag tatgttcaat tagcttttca attatgaata tatatatcaa ttattggtcg
     4561 cccttggctt gtggacaatg cgctacgcgc accggctccg cccgtggaca accgcaagcg
     4621 gttgcccacc gtcgagcgcc agcgcctttg cccacaaccc ggcggccggc cgcaacagat
     4681 cgttttataa attttttttt ttgaaaaaga aaaagcccga aaggcggcaa cctctcgggc
     4741 ttctggattt ccgatccccg gaattagatc ttggcaggat atattgtggt gtaacaagct
     4801 tcgtctcagt ca
//
