LOCUS       Exported                8541 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  bacterial pMCSG7 expression vector expressing Nme cas9 nickase with 
            a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag 
            and TEV site.
ACCESSION   .
VERSION     .
KEYWORDS    pMCSG7-D16A-NmeCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
  TITLE     DNase H Activity of Neisseria meningitidis Cas9.
  JOURNAL   Mol Cell. 2015 Oct 15;60(2):242-55. doi: 
            10.1016/j.molcel.2015.09.020.
  PUBMED    26474066
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        285..388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             389..1249
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(607..626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1420..2008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1909..1928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2162..2179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2194..2336
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2262..2281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2422..2444
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2438..2629)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3438..4520)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4482..4501
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4521..4598)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4804..4823
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4911..4930
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4911..4929
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4930..4954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4969..4991
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5002..5019
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5044..5064
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5071..8319
                     /codon_start=1
                     /product="Cas9 endonuclease from the Neisseria meningitidis
                     Type II CRISPR/Cas system (Esvelt et al., 2013)"
                     /label=NmCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MAAFKPNSINYILGLAIGIASVGWAMVEIDEEENPIRLIDLGVRV
                     FERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIK
                     SLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVA
                     GNAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDLQAELILLFEKQKEF
                     GNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLT
                     KLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKD
                     NAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRL
                     KDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNT
                     EEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRK
                     EIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLG
                     RLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEF
                     KARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKR
                     VFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAF
                     DGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAE
                     KLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKD
                     LEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQK
                     TGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQ
                     LIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILE
                     GIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
     CDS             8385..8402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8455..8473)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8469..8516
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      361 tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      481 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      541 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      661 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      901 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1561 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2041 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2161 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2221 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2281 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2341 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2401 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2461 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2521 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2581 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2641 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2701 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2761 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2821 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2881 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2941 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     3001 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     3061 tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg
     3121 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
     3181 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
     3241 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
     3301 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
     3361 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
     3421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt
     3541 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     3601 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     3661 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
     3721 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
     3781 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
     3841 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
     3901 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
     3961 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
     4021 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
     4081 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
     4141 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
     4201 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
     4261 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
     4321 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg
     4381 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg
     4441 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
     4501 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
     4561 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt
     4621 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     4681 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     4741 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     4801 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     4861 cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact
     4921 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
     4981 taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt agatctgggt
     5041 accgagaacc tgtacttcca atccaatgcc atggctgcct tcaaacctaa ttcaatcaac
     5101 tacatcctcg gcctcgctat cggcatcgca tccgtcggct gggcgatggt agaaattgac
     5161 gaagaagaaa accccatccg cctgattgat ttgggcgtgc gcgtatttga gcgtgccgaa
     5221 gtaccgaaaa caggcgactc ccttgccatg gcaaggcgtt tggcgcgcag tgttcgccgc
     5281 ctgacccgcc gtcgcgccca ccgcctgctt cggacccgcc gcctattgaa acgcgaaggc
     5341 gtattacaag ccgccaattt tgacgaaaac ggcttgatta aatccttacc gaatacacca
     5401 tggcaacttc gcgcagccgc attagaccgc aaactgacgc ctttagagtg gtcggcagtc
     5461 ttgttgcatt taatcaaaca tcgcggctat ttatcgcaac ggaaaaacga gggcgaaact
     5521 gccgataagg agcttggcgc tttgcttaaa ggcgtagccg gcaatgccca tgccttacag
     5581 acaggcgatt tccgcacacc ggccgaattg gctttaaata aatttgagaa agaaagcggc
     5641 catatccgca atcagcgcag cgattattcg catacgttca gccgcaaaga tttacaggcg
     5701 gagctgattt tgctgtttga aaaacaaaaa gaatttggca atccgcatgt ttcaggcggc
     5761 cttaaagaag gtattgaaac cctactgatg acgcaacgcc ctgccctgtc cggcgatgcc
     5821 gttcaaaaaa tgttggggca ttgcaccttc gaaccggcag agccgaaagc cgctaaaaac
     5881 acctacacag ccgaacgttt catctggctg accaagctga acaacctgcg tattttagag
     5941 caaggcagcg agcggccatt gaccgatacc gaacgcgcca cgcttatgga cgagccatac
     6001 agaaaatcca aactgactta cgcacaagcc cgtaagctgc tgggtttaga agataccgcc
     6061 tttttcaaag gcttgcgcta tggtaaagac aatgccgaag cctcaacatt gatggaaatg
     6121 aaggcctacc atgccatcag ccgtgcactg gaaaaagaag gattgaaaga caaaaaatcc
     6181 ccattaaacc tttctcccga attacaagac gaaatcggca cggcattctc cctgttcaaa
     6241 accgatgaag acattacagg ccgtctgaaa gaccgtatac agcccgaaat cttagaagcg
     6301 ctgttgaaac acatcagctt cgataagttc gtccaaattt ccttgaaagc attgcgccga
     6361 attgtgcctc taatggaaca aggcaaacgt tacgatgaag cctgcgccga aatctacgga
     6421 gaccattacg gcaagaagaa tacggaagaa aagatttatc tgccgccgat tcccgccgac
     6481 gaaatccgca accccgtcgt cttgcgcgcc ttatctcaag cacgtaaggt cattaacggc
     6541 gtggtacgcc gttacggctc cccagctcgt atccatattg aaactgcaag ggaagtaggt
     6601 aaatcgttta aagaccgcaa agaaattgag aaacgccaag aagaaaaccg caaagaccgg
     6661 gaaaaagccg ccgccaaatt ccgagagtat ttccccaatt ttgtcggaga acccaaatcc
     6721 aaagatattc tgaaactgcg cctgtacgag caacaacacg gcaaatgcct gtattcgggc
     6781 aaagaaatca acttaggccg tctgaacgaa aaaggctatg tcgaaatcga ccatgccctg
     6841 ccgttctcgc gcacatggga cgacagtttc aacaataaag tactggtatt gggcagcgaa
     6901 aaccaaaaca aaggcaatca aaccccttac gaatacttca acggcaaaga caacagccgc
     6961 gaatggcagg aatttaaagc gcgtgtcgaa accagccgtt tcccgcgcag taaaaaacaa
     7021 cggattctgc tgcaaaaatt cgatgaagac ggctttaaag aacgcaatct gaacgacacg
     7081 cgctacgtca accgtttcct gtgtcaattt gttgccgacc gtatgcggct gacaggtaaa
     7141 ggcaagaaac gtgtctttgc atccaacgga caaattacca atctgttgcg cggcttttgg
     7201 ggattgcgca aagtgcgtgc ggaaaacgac cgccatcacg ccttggacgc cgtcgtcgtt
     7261 gcctgctcga ccgttgccat gcagcagaaa attacccgtt ttgtacgcta taaagagatg
     7321 aacgcgtttg acggtaaaac catagacaaa gaaacaggag aagtgctgca tcaaaaaaca
     7381 cacttcccac aaccttggga atttttcgca caagaagtca tgattcgcgt cttcggcaaa
     7441 ccggacggca aacccgaatt cgaagaagcc gataccctag aaaaactgcg cacgttgctt
     7501 gccgaaaaat tatcatctcg ccccgaagcc gtacacgaat acgttacgcc actgtttgtt
     7561 tcacgcgcgc ccaatcggaa gatgagcggg caagggcata tggagaccgt caaatccgcc
     7621 aaacgactgg acgaaggcgt cagcgtgttg cgcgtaccgc tgacacagtt aaaactgaaa
     7681 gacttggaaa aaatggtcaa tcgggagcgc gaacctaagc tatacgaagc actgaaagca
     7741 cggctggaag cacataaaga cgatcctgcc aaagcctttg ccgagccgtt ttacaaatac
     7801 gataaagcag gcaaccgcac ccaacaggta aaagccgtac gcgtagagca agtacagaaa
     7861 accggcgtat gggtgcgcaa ccataacggt attgccgaca acgcaaccat ggtgcgcgta
     7921 gatgtgtttg agaaaggcga caagtattat ctggtaccga tttacagttg gcaggtagcg
     7981 aaagggattt tgccggatag ggctgttgta caaggaaaag atgaagaaga ttggcaactt
     8041 attgatgata gtttcaactt taaattctca ttacacccta atgatttagt cgaggttata
     8101 acaaaaaaag ctagaatgtt tggttacttt gccagctgcc atcgaggcac aggtaatatc
     8161 aatatacgca ttcatgatct tgatcataaa attggcaaaa atggaatact ggaaggtatc
     8221 ggcgtcaaaa ccgccctttc attccaaaaa taccaaattg acgaactggg caaagaaatc
     8281 agaccatgcc gtctgaaaaa acgcccgcct gtccgttaaa acattggaag tggataacgg
     8341 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
     8401 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     8461 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     8521 aaggaggaac tatatccgga t
//
