LOCUS       Exported                 844 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing 
            suppressor P19 plant expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pDGB3alpha2_35S:P19:Tnos  (GB1203)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(589..635)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(624..644)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 cgctttcttt ttcgaaggtt tcagtacctt cagggcatcc tcttgataca ttactttcca
       61 cttcgattgg ggcaagctgt agcagttctt gcttagaccg aattgccatc tcacagagat
      121 gctgaagagt tcgagagcct ccagaaacgg tgatactaac tcctcgaaac cgaatactat
      181 aggtacatcc gatctggtcg aaaccgaaaa atcgagatgc tgcatagtta accgaatctc
      241 ccgtccaaga tccaaggact ctgtgcagtg aagcttccgt cctgtcgtat ctgagatatc
      301 tcttaaatac aactttcccg aaaccccagc tttccttgaa accaagggga ttatcttgat
      361 tcgaattggt ctcatcgtta tgtagccgcc actcagtcca actcggactt tcgtcaggaa
      421 gtttgaaggg agaagtggta cctcctgatc ctccatccca acgttcactg ttagcttgtt
      481 ccctagcgtc gtttccttgt atagctcgtt ccatttcgac tagaatagta aattgtaatg
      541 ttgtttgttg tttgttttgt tgtggtaatt gttgtaaaaa taccggagtc ctctccaaat
      601 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
      661 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
      721 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
      781 gggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
      841 cctt
//
