LOCUS Exported 2752 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector. ACCESSION . VERSION . KEYWORDS pDGB1alpha1_SF (GB0106) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2752) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 2752) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2752 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 175..199 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" rep_origin complement(290..878) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(370..389) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1049..1864) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1772..1791 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 1856..1875 /label=pENTR-R /note="pENTR vectors, reverse primer" rep_origin 2155..2590 /label=pSa ori /note="origin of replication from bacterial plasmid pSa" misc_feature 2717..2739 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA (truncated)" ORIGIN 1 ggagtctcaa tggtaacttt actctttatt taaccataca ttttttttta tttttttcac 61 tttgttcttc atccactatt gttctttgtt catcttgaac aaaagctccc tccttctttg 121 ttcttcatcc accattgttc ttcatcaatc atttcgctgt catgagacga attctgacag 181 gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa 241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa 781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1021 gtatatatgt gtaacattgg tctagtgatt agaaaaactc atcgagcatc aaatgaaact 1081 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg 1141 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga 1201 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat 1261 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca 1321 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat 1381 caaccaaacc gttattcatt cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt 1441 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat 1501 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg 1561 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg 1621 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acaacattgg 1681 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc 1741 ggtagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat 1801 cagcatccat gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt tgaatatggc 1861 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata 1921 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttgttgaa 1981 taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc 2041 gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca 2101 tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcg atccccatcc 2161 aacagcccgc cgtcgagcgg gcttttttat ccccggaagc ctgtggatag agggtagtta 2221 tccacgtgaa accgctaatg ccccgcaaag ccttgattca cggggctttc cggcccgctc 2281 caaaaactat ccacgtgaaa tcgctaatca gggtacgtga aatcgctaat cggagtacgt 2341 gaaatcgcta ataaggtcac gtgaaatcgc taatcaaaaa ggcacgtgag aacgctaata 2401 gccctttcag atcaacagct tgcaaacacc cctcgctccg gcaagtagtt acagcaagta 2461 gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc gcccttggct 2521 tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc ggttgcccac 2581 cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt tataaatttt 2641 tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg gatttccgat 2701 ccccggaatt agatcttggc aggatatatt gtggtgtaac gaattcgtct ca //