LOCUS Exported 8113 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-fPV-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8113) AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM TITLE Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. JOURNAL Front Neural Circuits. 2009 . 3():19. PUBMED 19949461 REFERENCE 2 (bases 1 to 8113) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8113 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 48..177 /label=AAV2 ITR intron 2752..3227 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" primer_bind 3309..3325 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 3309..3325 /label=pBluescriptSK /note="For pBluescript vector" regulatory 3358..3367 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3364..4080 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3364..4080 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3409..3430) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3670..3689) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4017..4038 /label=EGFP-C /note="EGFP, forward primer" promoter complement(4141..4159) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4142..4159) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4179..4196) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4185..4296 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 4440..4569 /label=AAV2 ITR rep_origin 4940..5453 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5027..5046) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5237..5258 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5935..5954) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6054..6076 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6114..6132) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6200..6304 /gene="bla" /label=AmpR promoter CDS 6305..7165 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6523..6542) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7336..7924 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7825..7844 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8078..8095 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg cgcgctcgct 61 cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct 121 cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt 181 agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctaga ggatccttcg 241 aaaagcttgt atacggccgg ccacgcgtac aagatggata cgactgccag cttacccctg 301 cctggatctt cgttaattac catcagaaag actaaattct gctttaattc agtttctgat 361 acctattttt gagctgacac gagcttgtga aaacaactgc tggttgattg cgtgtttgca 421 ttgtcacatc tgtacagaat gtcagtggtt gcacaatttc tgggggggat tctccaaact 481 acagcgctgt tttaccggac tgagcaatgt caactacata ttaaaaagtt aaagggcaga 541 ttttctatct aacgtgcaga ttatgttgga tttaatcaag aacttcatag gactgttctc 601 tccttctgat tctcccataa ttcctacagt ctatgtgaca gcgaacacat catgctgccc 661 tcttttctca gccttgagcc tgtgtgtgtg tgtgtgtgtg tgcgtgtgtg tgtgtgtgtg 721 tgtgtgtgtt agtgatgggc tctggagctt ctctgaacag atatttcaga gcttcttacg 781 taacaggtcc cgctgcacct caatccttaa ctacagatgt gtcccagctg atccaaaacg 841 ggccatatga cttgatcagg tgttaacagt ggtggaaaaa accctgggag aggacacaag 901 cagaaaagag cagtgcatgg gtgaaagtaa aaaaaacttc atgcctgttt ttagaggtaa 961 taatggagca tgtattgatt tatgtggagt gtgtaggtgt gagcatcatt atttaatcaa 1021 ccttccctgt gggtgctgcc ctgtgattgc tgatgtaatg aggtaccaaa gcatcaatat 1081 ctggaggtag ctctgcaaac atgcgatgga agcatgaaaa tctgcctcgg tttacagtct 1141 cgggccgcct ctcagcactg ggatggatca cactatttga ttgaaggcac atgtgccaca 1201 gctctgcaaa ggtggagcaa cctgcacatt agctcttaag tcacaaatca ctctgcaaaa 1261 gtctaaaatc accgagaatg ataaaacttt taaaatgcag agatcctgaa aaaacacaag 1321 gcataaaaaa gaaagacaaa tatgttgtct ccatagtaac catatctcta ataaagaccc 1381 gaccatgcag atagttttga ccttgatata ttttttaggt tgattacaac ctctgaaatc 1441 ctaaatgttg gtgctaaaaa gatttaattt taactatctg taccagggga aggtggacgg 1501 tttggcctct cattacttaa cggtttggcc gtctgacaac caaacgccca acaacttggt 1561 ggattccttt taaaaccgca ccatctccca cgcacgcccc cacgcagcag ccgtaacatt 1621 agcactaaat ataccactct aaccagtgcc tgcagtgcag tcgtgcatgt ttggcaaaga 1681 gtggggcttc cttctcccac tctgctcagc caactgcctg accaggacct acggggtcgc 1741 gggagggttt gtgccgtgca gttcttgcca tctcggttct ggtcagtttt gtcaactata 1801 ttgtaatcac cgtgattgac aggtgaactc tggttgcaca gaaggaggtt gaactgcaca 1861 gaaggagaac ttcagctcag actgctcacg ctggcatgcc tcgatctaaa ataaactctc 1921 tccaactttt ctttccttga cattgtaggt taaatcagaa gtctgagctc tgctctgaac 1981 tttccaactc tttctttctg tcattattga gaagcgtctc tataaaacca attagactta 2041 aacatacata catacataca tacatacata catacataca tacatacata catacataca 2101 tacatacata tggcatgtga tcaaacatat gtgatatctt tgtctgagat gcctgagcct 2161 aggaacaggt tagagggcca gaacatcgtg acatacctga actgtctgtg gtaacttaat 2221 caaagataac tgagctgcaa tcactgggca gtttcagcat cttctcacag cccagagcac 2281 ttcagcaaac acttgttaaa gaagaaatta gactctccaa agacttctgt gtcataatca 2341 tgaagacaag agatattttg gcttcgattt gtacatcatt acctgtactt tgaagggata 2401 aaaataaaac aaaagcttca acatctggtc agcacacatg gttcggtctc tgtgtacagt 2461 cgcagacggc tcaggcctga cccctatcga cacctgtctg ctactacaac agatctatta 2521 cactcttcgc agttaaaaat gatttacttc atttaaaaaa agtccgccat ccaacaaaac 2581 gtgtgttctg gcatgcagat caaacatcag attagggatt tttgtacact accttcctca 2641 gttacagctg atatatatct tttctgcttt tccactgacc tttaacctcc tcctgtcttt 2701 tcagctggca aagctagcgg cgcgccgagc tcggatcctg agaacttcag ggtgagtcta 2761 tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag 2821 gggagaagta acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt 2881 aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct 2941 aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa 3001 agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt 3061 ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca 3121 gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct 3181 aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg 3241 tgctggtctg tgtgctggcc catcactttg gcaaagcacg tgaattcgaa gatctggccg 3301 cctcggcctc tagaactagt ggatcccccg ggctgcagga attcgatgct accggtcgcc 3361 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 3421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 3481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 3541 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 3601 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 3661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 3721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 3781 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 3841 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 3901 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 3961 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 4021 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 4081 tccggactca gatcctacta gtaggatctc gagtttaaac cgtacgggaa acggccctat 4141 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 4201 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 4261 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 4321 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcgc 4381 ggccgctcta gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa 4441 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 4501 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg 4561 agcgcgcagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 4621 cagcctgaat ggcgaatggc gattccgttg caatggctgg cggtaatatt gttctggata 4681 ttaccagcaa ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc 4741 aaagaagtat tgcgacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc 4801 tcactgatta taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt 4861 taatcggcct cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc 4921 tcgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 4981 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 5041 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 5101 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 5161 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 5221 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 5281 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 5341 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaaata 5401 tttgcttata caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg 5461 attgacatgc tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca 5521 ggcaatgacc tgatagcctt tgtagagacc tctcaaaaat agctaccctc tccggcatga 5581 atttatcagc tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt 5641 ctcacccgtt tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg 5701 gttctaaaaa tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg 5761 gtcataatgt ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt 5821 ttgctaattc tttgccttgc ctgtatgatt tattggatgt tggaatcgcc tgatgcggta 5881 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 5941 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 6001 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 6061 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 6121 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 6181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 6241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 6301 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 6361 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 6421 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 6481 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 6541 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 6601 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 6661 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 6721 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 6781 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 6841 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 6901 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 6961 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 7021 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 7081 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 7141 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 7201 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 7261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 7321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 7381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 7441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 7501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 7561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 7621 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 7681 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 7741 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 7801 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 7861 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 7921 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 7981 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 8041 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 8101 ggaagagcgc cca //