LOCUS       Exported                7999 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hPDGFRA-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009  . 3():19.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     27..44
                     /label=L4440
                     /note="L4440 vector, forward primer"
     intron          2868..3343
                     /label=-beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     3425..3441
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3425..3441
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      3474..3483
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3480..4196
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3480..4196
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3525..3546)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3786..3805)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4133..4154
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4257..4275)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4258..4275)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4295..4312)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4301..4412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4888..5401
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4975..4994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5185..5206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5883..5902)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6002..6024
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6062..6080)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6148..6252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6253..7113
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6471..6490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7284..7872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7773..7792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgagcgcgca gagagggagt
       61 ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc
      121 tacgtagcca tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgttca
      181 ggagggcaag gtttctagcc ccctgcggct ccgggaccac ccaggacccg gggcgtcgtg
      241 ggaccgatct cgaaggatct tagaacgaca ctgggagagc gcggcaagaa ctagccccac
      301 cttcccccga cgccccggac tgactgccgg gcgcagaccc gacttctccc gaaccagcct
      361 cggaccagcc ttagaaggcg gaaaacagga ttcaaacccg cctatgagca gtggtagggg
      421 cgggggctgt gggggcagga ctcggtttaa ggaaccaacc gcctcggttc gagggcctcc
      481 tgtccggagg ttgctcctga ccactgggtt tattttgctt ttgcagagga ggcagatgtt
      541 gacctctctt ctttgcacag tctctccgca aaattttccc ccaaccattt gctttcttgc
      601 gccacccccc accggggcac tatccctgga gtcagctcct caggccttta aaccttccag
      661 aatgtcacac atggaaacct ttagcaaatg tttgttaatg atcataacaa aggcatcatt
      721 caaattaggc aggtaattac taccagaagg acaactgggt gctcgcttgc tcatccattg
      781 ctttctgctt taaactcccg aggagtcgct gcctggagac actcccgccc ggactgcttc
      841 tcgggcccca ttaaacggct ctgaaatgaa ctgtgaagtt accatttgtg ggcggagagc
      901 gctggggccc tgcggacgga agaccctggc ttgaccggac taacagtgtc gccgaagcaa
      961 agcagcccct ctgggtaggc tgtctgtctc gcctcctacc ctctggggca actagcctaa
     1021 aaacccggtt ctcaacttaa cagcatgtgg cccagcaccc gcttttgcct tttattccgc
     1081 actctgattt ttggggggat cgtttgttcc cgctcatttt ctacaaacat tacggtatcc
     1141 tgttagcatt ccgaacaagg ggctgttcat atatttgcct tcaatgattt cctgaaggga
     1201 catgtggaag taatagcgag cggtgcagtc acccaagctg aagatgcacg agagcgggtc
     1261 gcgtagaaga actgcggcaa tgggaccccc agcgcagccc caggcgccct gttttcgagg
     1321 ctgacatccc aagatcctgt cctggttagg cacagcaccg cgcccgttgg agtacttctc
     1381 tgtgactctc ccctgtccac tgcactgtgt ccaccctcct ggtgaaggag gagacggctg
     1441 ttggacatgc cagtcccaac ctggccactt tggccatttt cagagtgcgt gtgttggggt
     1501 ggggttgagg gagaaggatg aaggatgacc cccaacccca acaggagaat tcaaaaatca
     1561 gagagcgatg aaggtgaacg cgctcccctc ccccgctgtc gctgggaagc cccactctgc
     1621 ccaggtctgg ttcctggagc atcagcgccc cctttccctc gccggcagga tcgtttcctc
     1681 ccgacactgg cctctcgcct tgggcgcagg gtttggggct cagctgtcaa ttccaaggaa
     1741 ttcgtagggt tcgacccacg cggcgggagg gaacggaggg agaatcaaaa ggcatcctcc
     1801 cccacgacgc ggaggcggtg gtacgattcc tcaaacgccc aagcttgagc tttttttgta
     1861 tgcgagatag aagccagggc aacctcgccc agataacccc gaaaacaaag gcacgaccag
     1921 ataagtgact tcaagggagt aaaggtcgtt ctatcggaga acgttccaaa cctacacaga
     1981 ccctcttttc tttgtaccgt atttcagacc acccagtctt gtacacacac acacaccaca
     2041 caaaacaaaa acccatcatt gtcatattgg actcaacagt ttgcccaatc ctattaaata
     2101 actagcaact atatagccct tcccctaaaa gacccctagt tcaaaacggc gcaaccgctt
     2161 ggagatttct cccgagggcc ctatttctcg ttgggccgag tttgtagaag ggacatttct
     2221 tgattctaga tttatattct ctctgggtat taaatgcaat tttgtatgtt ccttttcttc
     2281 ttttcaaaga gaatacaaaa ctatagcagt ctaagaaaat aaccccaaaa tggggaggca
     2341 aactcattct ggaaatgatg ggctgtttgt agtttcacaa aacctctttc ccggcagagc
     2401 accaacacct cccccttcca ccacccccca tcccatctgg tctgcttctc cccgcccccc
     2461 agttgttgtc gaagtctggg ggttgggact ggaccccctg attgcgtaag agcaaaaagc
     2521 gaaggcgcaa tctggacact gggagattcg gagcgcaggg agtttgagag aaacttttat
     2581 tttgaagaga ccaaggttga gggggggctt atttcctgac agctatttac ttagagcaaa
     2641 tgattagttt tagaaggatg gactataaca ttgaatcaat tacaaaacgc ggtttttgag
     2701 cccattactg ttggagctac agggagagaa acagaggagg agactgcaag agatcattgg
     2761 aggccgtggg cacgctcttt actccatgtg tgggacattc attgcggaat aacatcggag
     2821 gagaaggtgc tagcggcgcg ccgagctcgg atcctgagaa cttcagggtg agtctatggg
     2881 acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga
     2941 gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa
     3001 aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc
     3061 tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa
     3121 taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc
     3181 atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta
     3241 ccattctgct tttattttat ggttgggata aggctggatt attctgagtc caagctaggc
     3301 ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct
     3361 ggtctgtgtg ctggcccatc actttggcaa agcacgtgaa ttcgaagatc tggccgcctc
     3421 ggcctctaga actagtggat cccccgggct gcaggaattc gatgctaccg gtcgccacca
     3481 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3541 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3601 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3661 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     3721 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3781 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3841 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3901 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3961 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4021 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4081 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4141 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
     4201 gactcagatc ctactagtag gatctcgagt ttaaaccgta cgggaaacgg ccctattcta
     4261 tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     4321 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4381 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4441 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcgcggcc
     4501 gctctagagc atggctactg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4561 cagttgcgca gcctgaatgg cgaatggcga ttccgttgca atggctggcg gtaatattgt
     4621 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     4681 tactaatcaa agaagtattg cgacaacggt taatttgcgt gatggacaga ctcttttact
     4741 cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa
     4801 aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt
     4861 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4921 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4981 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5041 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5101 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5161 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5221 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5281 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5341 tttaaatatt tgcttataca atcttcctgt ttttggggct tttctgatta tcaaccgggg
     5401 tacatatgat tgacatgcta gttttacgat taccgttcat cgattctctt gtttgctcca
     5461 gactctcagg caatgacctg atagcctttg tagagacctc tcaaaaatag ctaccctctc
     5521 cggcatgaat ttatcagcta gaacggttga atatcatatt gatggtgatt tgactgtctc
     5581 cggcctttct cacccgtttg aatctttacc tacacattac tcaggcattg catttaaaat
     5641 atatgagggt tctaaaaatt tttatccttg cgttgaaata aaggcttctc ccgcaaaagt
     5701 attacagggt cataatgttt ttggtacaac cgatttagct ttatgctctg aggctttatt
     5761 gcttaatttt gctaattctt tgccttgcct gtatgattta ttggatgttg gaatcgcctg
     5821 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     5881 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5941 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6001 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     6061 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6121 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6181 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6241 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6301 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6361 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6421 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6481 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6541 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6601 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6661 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6721 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6781 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6841 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6901 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6961 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7021 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7081 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7141 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7201 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7981 tttgagtgag ctgataccg
//
